Gradient Preview Functionality - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC

Gradient Preview Functionality

The gradient preview provides the following functions:
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Reference Signal
The user can load a chromatographic signal from an LC detector - a so-called
reference signal. The signal will be shown in the gradient preview. This signal is
automatically shown in the gradient preview of the setup dialog as long as the
reference signal is part of the method. The signal can also be removed from the
method or replaced by another signal.
Loading / removing a reference signal is triggered by toolbar buttons (or the
context menu) of the gradient preview.
When a reference signal is loaded, a reference signal related y-axis is shown on
the right side of the gradient preview window. The grid of the graphic window is
either adjusted to the absorbance axis (right axis) or the %B axis (left) and can be
changed by clicking on the corresponding axis. The signal name (e.g. DAD1 A,
Sig= 280, 190 Ref=550,100) is shown in the legend of the graphic window.
Figure 60
The reference signal offers the following:
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2D-LC - User Guide
Displays the gradient (%B) of the 1
Displays the gradient (%B) of the 2
dimension, depending on the selected 2D-LC mode (comprehensive OR
heart-cutting)
Allows to graphically edit the gradient shifting
Displays a reference signal by which a user can easily setup the trigger table
or optimize his peak detector settings
Gradient preview
Simplified set-up of (time-based) heart cuts in case the chromatogram of the
sample is known in advance
Preview of peaks, which would be analyzed in the 2
on the current peak detector settings (threshold, slope)
st
dimension pump
nd
dimension during the runtime of the first
nd
dimension that is based
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