Optimizing The Dilution By Using Asm Capillaries; Optimizing The Sample Loop Flush - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Development of Active Solvent Modulation (ASM)

Optimizing the dilution by using ASM capillaries

A choice of four different ASM capillaries is available for achieving best results.
Longer capillaries reduce, shorter capillaries increase the dilution of
the sample loop.
Install and configure different ASM capillaries (see
page 190) for optimizing the results.
Capillary p/n Length (mm)
5500-1300 85
5500-1301 170
5500-1302 340
5500-1303 680

Optimizing the sample loop flush

Activate ASM in the software and set Flush sample loop to 3.0 times.
Flushing the sample loop 3 times is typically enough and the recommended
NOTE
default. Less time may be sufficient and can be verified during optimization. The
user interface displays how long this will take.
Figure 70
2D-LC - User Guide
Inner diameter Volume
(mm) (µl)
0.12 0.96
0.12 1.9
0.12 3.8
0.12 7.7
Set Flush sample loop (example)
"Configure the ASM Valve"
ASM factor Split ratio
(loop:ASM)
5 1:4
3 1:2
2 1:1
1.5 1:0.5
1
D solvent in
on
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