Related Manuals for Agilent Technologies 1260 Infinity II Quaternary System
Summary of Contents for Agilent Technologies 1260 Infinity II Quaternary System
- Page 1 Agilent InfinityLab LC Series 1260 Infinity II Quaternary System Manual and Quick Guide Agilent Technologies...
- Page 2 Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 3 This chapter provides information on running an Agilent 1260 Infinity II LC System. 4 Parts and Consumables This chapter provides information on additional parts and consumables. 5 Appendix This chapter provides addition information on safety, legal, and web. 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 4: Table Of Contents
3 Quick Start Guide Best Practices Prepare a Run Check Out the System 4 Parts and Consumables Tool Kit A-Line Quick Connect and Quick Turn Fittings 5 Appendix Safety Agilent Technologies on Internet 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 5 1260 Infinity II Quaternary System - Manual and Quick Guide Introduction Product Description Features of the Agilent 1260 Infinity II Quaternary LC System Components Quaternary Pump G7111B Quaternary Pump VL G7111A Isocratic Pump G7110B Multisampler G7167A Vialsampler G7129A Multicolumn Thermostat G7116A...
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Page 6: Introduction Product Description
The designed for method transferability and stepwise upgrade capability enables a risk-free integration in current infrastructure matching your budget. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 7: Features Of The Agilent 1260 Infinity Ii Quaternary Lc
1260 Infinity II DAD HS Detector delivers lower detection limit and higher data quality for more confidence. Seamless integration in your chromatography data system: Agilent’s Instrument Control Framework (ICF) enables smooth control of Agilent LC instrumentation through third-party chromatography data systems. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 8: System Components
The Agilent 1260 Infinity II LC is described in more detail in the following sections. All modules (except the optional ELSD) are stackable, see “Optimizing the Stack Configuration” on page 24 For specifications, please refer to the individual module user documentation. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 9: Quaternary Pump G7111B
(especially in the food, environmental and pharmaceutical sectors) as well as for method development. Figure 1 Overview of the pump 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 10: Quaternary Pump Vl G7111A
(especially in the food, environmental and pharmaceutical sectors) as well as for method development. Figure 2 Overview of the pump 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 11: Isocratic Pump G7110B
The Agilent 1260 Infinity II Isocratic Pump is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision in retention times is crucial. Figure 3 Overview of the pump 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 12: Multisampler G7167A
With the multi-wash capability, you can reduce carryover to less than 9 parts per million. Figure 4 Overview of the Multisampler 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 13: Vialsampler G7129A
LC columns with temperature control up to 80 °C as well as a sample cooler for stable temperatures down to 4 °C, all within one module. Figure 5 Overview of the Vialsampler 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 14: Multicolumn Thermostat G7116A
The MCT fits with all 1260 Infinity II modules and can also be combined with modules of the Agilent 1260 and 1290 Infinity II Series. Figure 6 Overview of the Multicolumn Thermostat 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 15: Diode Array Detector Hs G7117C
For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 120 Hz. Figure 7 Overview of the detector 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 16: Diode Array Detector Wr G7115A
The built-in holmium oxide filter features the fast wavelength accuracy verification, while the built-in temperature controls improves the baseline stability. Additional diagnostic signals for temperature and lamp voltage monitoring are available. Figure 8 Overview of the detector 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 17: Variable Wavelength Detector G7114A
Low detector noise (<±2.5 μAU) and baseline drift (<1·10 AU/h) facilitates precise quantification of trace levels components. High productivity can be achieved with fast analysis at up to 120 Hz data rates. Figure 9 Overview of the detector 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 18: Fluorescence Detector G7121A
High-speed detection with up to 74 Hz data rates keeping you pace with the analysis speed of fast LC. Figure 10 Overview of the detector 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 19: Refractive Index Detector G7162A
Infinity II RID is also the detector of choice for gel permeation chromatography (GPC) or size exclusion chromatography (SEC). Status indicator Interface port area Service door Power switch Leak drain Figure 11 Overview of the detector 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 20: Solutions
SPE cartridges and inject directly onto the analytical column. With this approach you can use your system either for online SPE analysis or for a direct injection without re-plumbing the system. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 21 Introduction Solutions Figure 12 Position 1 Figure 13 Position 2 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 22: 1260 Infinity Ii Multimethod Solution
• A solvent selection valve attached to the LC stack allows additional access of up to 12 different solvents. • Utilization and productivity of the LC are optimized by switching between several applications run on a single instrument. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 23 Introduction Solutions Figure 14 Hydraulic flow path schematics for a 4-column selection setup Figure 15 Hydraulic flow path schematics for a 4-column selection setup 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 24: Optimizing The Stack Configuration
Agilent recommends to use the A-Line LC Flex Bench for all Agilent-LC-Systems. Main features: • Increases flexibility in the lab • Safe moving of LC • Easy stack customization • Included waste management 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 25 Introduction Optimizing the Stack Configuration Figure 16 Agilent A-Line LC Flex Bench 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 26: One Stack Configuration
26. This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required. Figure 17 Single stack configuration (bench installation, example shows a multisampler) 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 27 Introduction Optimizing the Stack Configuration Vialsampler stack configurations with integrated column compartment Figure 18 Single stack configuration (bench installation, example shows a vialsampler with optional ICC installed) 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 28: Two Stack Configuration
To avoid excessive height of the stack (for example when using the system in combination with an additional detector) it is recommended to form two stacks. Figure 19 Two stack configuration (bench installation, example shows a multisampler) 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 29 Introduction Optimizing the Stack Configuration Vialsampler Two Stack Configurations Figure 20 Two stack configuration (bench installation, example shows a vialsampler) 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 30: Leak And Waste Handling
• from the detector's flow cell outlet • from the Multisampler needle wash port • from the Sample Cooler (condensate) • from the Seal Wash Sensor • from the pump's Purge Valve or Multipurpose Valve 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 31 Introduction Leak and Waste Handling Figure 21 Infinity II Leak Waste Concept (flexible rack installation) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 32 Introduction Leak and Waste Handling Figure 22 Infinity II Single Stack Leak Waste Concept (bench installation) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 33 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container. 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 34: Waste Guidance
Leak and Waste Handling Waste Guidance The waste drainage must go straight into the waste containers. The waste flow must not N O T E be restricted at bends or joints. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 35: Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 36 Introduction Leak and Waste Handling 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 37: Configuration Settings
1260 Infinity II Quaternary System - Manual and Quick Guide Configuration Settings General Information on LAN Configuration Instrument Configuration Lab Advisor Installing Add-ons This chapter describes how to configure the system. Agilent Technologies... -
Page 38: General Information On Lan Configuration
General Information on LAN Configuration LAN configuration is executed from the module with direct LAN connection to the controller software. This must be the module (usually the detector) with the highest data rate. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 39: Instrument Configuration
All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP. 2 Enter the setup information (MAC / IP address and/or Instrument Name). a Agilent OpenLab ChemStation (Configure Instrument): 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 40 Configuration Settings Instrument Configuration b Lab Advisor (Instrument Overview - Add Instrument): 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 41: Lab Advisor
Configuration Settings Lab Advisor Lab Advisor 1 In the Action Panel of the System Overview, click Add System. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 42 3 Enter the connection details in the Instrument Address field. The Instrument Address can be an IP address, the host name or, if you are connecting N O T E using a serial cable, the COM port. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 43 As soon as you add a second instrument, the System Name field is activated to allow you N O T E to edit the system name. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 44 6 Click OK to finish adding the system and close the Add System dialog box. The system becomes visible in the System Overview, and Lab Advisor tries to connect to it. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 45 1 In the Global Tasks section of the Navigation Panel, click Configuration. The Configuration screen is displayed. 2 Click Add-ons to navigate to the Configuration - Add-ons screen. Figure 24 Add-ons in Configuration 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 46 Lab Advisor shuts down and the Add-on installation is started. When the installation is finished, the newly installed Add-on is included in the table in the Configuration - Add-ons screen. 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 47: Quick Start Guide
1260 Infinity II Quaternary System - Manual and Quick Guide Quick Start Guide Best Practices Prepare a Run Check Out the System Checkout Method Setup the Checkout Method This chapter provides information on running an Agilent 1260 Infinity II LC System. -
Page 48: Best Practices
For best practices, refer to the Agilent Information Center on Agilent InfinityLab LC Series N O T E User Documentation (G4800-64600) or the 1290 Infinity Pump Quick Reference Sheet (01200-90091). 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 49: Prepare A Run
Regularly check the filling level of the waste container. The residual free volume in the waste container must be large enough to collect the waste liquid. ➔ To achieve maximal safety, regularly check the tubing for correct installation. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 50 2 Fill the solvent bottles with adequate solvents for your application. 3 Place solvent tubings with bottle head assemblies into the solvent bottles. 4 Place solvent bottles into the solvent cabinet. 5 Solvent bottle filling dialog (in the software). 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 51 Prime the pump (after installation of the system). For details on priming and purging, refer to the technical note Best Practices for Using an N O T E Agilent LC System . 7 Change solvent (if necessary). 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 52 Quick Start Guide Prepare a Run 8 Choose the tray format of the sampler. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 53 Quick Start Guide Prepare a Run 9 Add a new column. 10 Enter the column information. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 54 Quick Start Guide Prepare a Run 11 Select the column position. 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 55 12 Set the detector according to the needs of your method. For details on running a method, see “Setup the Checkout Method” on page 61 as an N O T E example. 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 56: Check Out The System
The Agilent isocratic checkout sample (01080-68704) serves as standard for systems with 400 bar and contains each of four components dissolved in methanol. • Dimethylphthalate • Diethylphthalate • Biphenyl • o-Terphenyl 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 57 Poroshell 120 EC-C18, Poroshell 120 EC-C18, Poroshell 120 EC-C18, 4.6 x 100 mm, 4 µm 4.6 x 50 mm, 2.7 µm 4.6 x 150 mm, 4 µm 695970-902T 699975-902T 693970-902T 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 58 Value Flow 1 mL/min Solvent A Water Solvent B Compressibility Composition 65 % B (ACN) Composition 35 % A (Water) Stoptime 10 min Pressure Limit 400 bar Minimum Stroke Automatic 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 59 1 µL Stoptime as pump Draw speed 100 µL/min Table 7 Checkout method parameter settings Multicolumn Thermostat (G7116A) Parameter Value Temperature (left) 40 °C Temperature (right) combined Stoptime as pump 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 60 Stoptime as pump Spectrum None Autobalance Prerun Table 9 Checkout method parameter settings Variable Wavelength Detector (G7114A) Parameter Value Wavelength 254 nm Peakwidth 40 Hz Stoptime as pump Autobalance Prerun 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 61: Setup The Checkout Method
The setup of the checkout method in this procedure is an example. For the individual N O T E module parameters, see “Checkout Method” on page 56. 1 Turn on the lamp. 2 Load the the default method DEF_LC.M 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 62 Quick Start Guide Check Out the System 3 Change the method and timetable settings for the 1260 Infinity II Quaternary Pump (G7111B) Figure 25 Method settings 1260 Infinity II Quaternary Pump (G7111B) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 63 Quick Start Guide Check Out the System Figure 26 Timetable settings 1260 Infinity II Quaternary Pump (G7111B) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 64 Quick Start Guide Check Out the System 4 Change the method settings for the 1260 Infinity II Multisampler (G7167A) Figure 27 Method setting 1260 Infinity II Multisampler (G7167A) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 65 Quick Start Guide Check Out the System Figure 28 Method setting 1260 Infinity II Multisampler (G7167A) - Injection Path Cleaning 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 66 Quick Start Guide Check Out the System 5 Change the method settings for the 1260 Infinity II Multicolumn Thermostat (G7116A) Figure 29 Method setting 1260 Infinity II Multicolumn Thermostat (G7116A) 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 67 Method setting 1260 Infinity II Diode Array Detector WR (G7115A) 7 Save the method as GRAD-1.M 8 Equilibrate the system for 10 min under checkout conditions 9 Run and evaluate the checkout method 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 68 Quick Start Guide Check Out the System 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 69 1260 Infinity II Quaternary System - Manual and Quick Guide Parts and Consumables Tool Kit A-Line Quick Connect and Quick Turn Fittings A-Line Quick Connect Fittings A-Line Quick Connect Fitting Replacement Capillaries A-Line Quick Turn Fitting Capillaries for use with the A-Line Quick Turn Fitting This chapter provides information on additional parts and consumables.
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Page 70: Parts And Consumables Tool Kit
Tool Kit The HPLC System Tool Kit contains all tools to operate and maintain a 1260 Infinity II LC system. The High quality tools are packed in an appealing tool case. 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 71 Parts and Consumables Tool Kit Figure 31 Tool kit 1260 Infinity II Quaternary System - Manual and Quick Guide...
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Page 72: A-Line Quick Connect And Quick Turn Fittings
A-Line Quick Connect Assy ST 0.17 mm x 150 mm 5067-6168 A-Line Quick Connect Assy ST 0.17 mm x 220 mm 5067-6169 A-Line Quick Connect Assy ST 0.17 mm x 280 mm 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 73: A-Line Quick Connect Fitting Replacement Capillaries
A-Line Capillary ST 0.17 mm x 150 mm 5500-1183 A-Line Capillary ST 0.17 mm x 220 mm 5500-1230 A-Line Capillary ST 0.17 mm x 280 mm 5500-1231 A-Line Capillary ST 0.17 mm x 500 mm 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 74: A-Line Quick Turn Fitting
Parts and Consumables A-Line Quick Connect and Quick Turn Fittings A-Line Quick Turn Fitting Figure 33 A-Line Quick Turn Fitting Description 5067-5966 A-Line Quick Turn fitting 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 75: Capillaries For Use With The A-Line Quick Turn Fitting
5500-1235 Capillary ST 0.17 mm x 380 mm, long socket 5500-1236 Capillary ST 0.17 mm x 400 mm, long socket 5500-1197 Capillary ST 0.17 mm x 500 mm, long socket 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 76 Parts and Consumables A-Line Quick Connect and Quick Turn Fittings 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 77 1260 Infinity II Quaternary System - Manual and Quick Guide Appendix Safety General Safety Information Safety Standards General Before Applying Power Ground the Instrument Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover Do Not Modify the Instrument...
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Page 78: Safety
Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 79: Before Applying Power
Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔... -
Page 80: Do Not Operate In An Explosive Atmosphere
Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 81: Solvents
For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 82: Symbols
A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 83: Appendix Safety
➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. 1260 Infinity II Quaternary System - Manual and Quick Guide... -
Page 84: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1260 Infinity II Quaternary System - Manual and Quick Guide... - Page 85 VL description product detector diode array prepare fluorescence safety class I host name safety general information symbols instrument address system name instrument name instrument type internet thermostat IP address multicolumn 1260 Infinity II Quaternary System - Manual and Quick Guide...
- Page 86 In This Book This manual contains technical reference information about the Agilent InfinityLab LC Series 1260 Infinity II Quaternary System. The manual describes the following: • introduction, • product description, • best practices, • system optimization, • quick start guide.