Checkout/Familiarization Procedure - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

6 Run the System
Multiple Heart-Cutting and High Resolution Sampling 2D-LC

Checkout/Familiarization Procedure

Checkout runs - MHC: 1290 Infinity Binary or Quaternary LC in
Parts required p/n
5190-6895
858700-902
857768-901
G2453-85060
Hardware required "Multiple Heart-Cutting Configuration"
Software required CD
Preparations Solvents needed:
•
•
Preparations:
1
2
3
2D-LC - User Guide
Description
2D-LC starter sample, 1 x 2 mL
Includes 2 mL
RRHD SB-C18, 2.1x100 mm, 1.8 µm, 1200 bar
1
D
RRHD Bonus-RP, 2.1x50 mm, 1.8 µm, 1200 bar
2
D, Heart-cutting
Formic Acid-Reagent Grade 5 mL (5 cc)
1
D: mobile phase A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060), B
= methanol
2
D: mobile phase A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060), B
= acetonitrile
Prepare dilution solvent (20 MeOH in mobile phase A): Add 300 µL MeOH to 1200 µL Mobile
Phase A.
Prepare 400 µL sample: Add 40 µL 2D-LC starter sample to 360 µL dilution solvent.
Load method Checkout_MHC_Comp_1290BinX1290Bin.M from the 2D-LC Addon SW CD and
modify the settings for your single heart or multiple heart cutting configuration.
on page 61
1
D
163