Set Cuts In The Software (Hires Sampling Only) - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC

Set cuts in the software (HiRes Sampling only)

Peak parking steps for high-resolution sampling
NOTE
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Transfer volume and sampling time
NOTE
The volume of the capillary between 2D-LC valve and each deck valve
determines the minimum sampling time.
t =
This is the time t, which is needed for filling the transfer volume V at a given
flow rate F.
If the sampling time would be less than that, two cuts could end up in the same
transfer capillary.
2D-LC - User Guide
Flow goes through loop 1 in deck A.
Cut 1 detected. Valve in deck A switches at beginning of
cut 1 to loop A2 and is parked there,
cut 2 to loop A3
cut 3 to loop A4
cut 4 to loop A5
Cut 5 is on its way (in the transfer capillary) to loop A6 – the last loop in deck
A. After switching the 2D-LC valve, the cut will be analyzed immediately.
Cut 6 goes to loop 1 in deck B, transfer requires time, which needs to be
configured:
Cut 7 goes to loop B2,...,
cut 10 goes to loop B5.
Last loop is required for flow-through while other deck runs analysis.
During analysis, loops are filled with solvent of
5 mL
V
F ; for example 500 pL/ min
2
D gradient base.
= 0.6 s
1
D
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