Agilent Technologies InfinityLab LC Series User Manual
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Agilent Technologies InfinityLab LC Series User Manual

Preparative open-bed fraction collector
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1290 Infinity II
Preparative Open-Bed Fraction Collector
Agilent InfinityLab LC Series
User Manual

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  • Page 1 1290 Infinity II Preparative Open-Bed Fraction Collector Agilent InfinityLab LC Series User Manual...
  • Page 2 Agilent Technologies, Inc. as understood and met. furnishing, use, or performance of this governed by United States and interna- document or of any information con- tional copyright laws.
  • Page 3 In This Guide This manual contains technical reference information about the Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector (G7159B). Introduction This chapter gives an introduction to the module and an instrument overview. Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
  • Page 4 Identifying Cables This chapter provides information on cables used with the module. Hardware Information This chapter describes the module in more detail on hardware and electronics. Appendix This chapter provides additional information on safety, legal, and web. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 5: Table Of Contents

    Contents Introduction Intended Use Product Description Features Overview of the Module Fraction Collector Principle Leak and Waste Concept Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Using the Fraction Collector Warnings and Cautions Solvent Information Turn on/off Status Indicators Drawer Status Indicator Exchange Drawers Exchange Containers...
  • Page 6 Preparing the Fraction Collector Best Practices Capillary Color Coding Guide Swage Fittings Setting up the Fraction Collector with the Instrument Control Interface Method Parameter Settings Pooling Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Error Information What Are Error Messages General Error Messages Maintenance Introduction to Maintenance...
  • Page 7 Electrical Connections Interfaces Setting the 6-bit Configuration Switch Early Maintenance Feedback Instrument Layout Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 8: Introduction

    Introduction Intended Use Product Description Features Overview of the Module Fraction Collector Principle Leak and Waste Concept Leak Sensor This chapter gives an introduction to the module and an instrument overview. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 9: Intended Use

    Introduction Intended Use Intended Use The 1290 Infinity II Preparative Open-Bed Fraction Collector is designed to be used in combination with other InfinityLab LC modules in a preparative-scale HPLC system to separate and purify inorganic or organic compounds via reversed-phase chromatography applications. The module can collect fractions of desired volume for sample purification or for later analysis.

  • Page 10: Product Description

    Introduction Product Description Product Description The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector is the ideal solution that automatically manages high-capacity fraction collection of purified peaks for semi-preparative and preparative scale purification. Collect milligrams to multi-gram quantities of sample intuitive with ease. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector features handling a wide variety of collection vessels with ease and capacity of up to 5.9 L by using 150 x 25 mm (L x OD).

  • Page 11: Features

    Introduction Features Features • Maximize your flexibility – Choose how much you want to collect, whether you prefer a large number of fractions which enables collection of up to 432 fraction in 150 x 12 mm (L x OD) vessels, or you prefer large volumes, or any combination of formats.

  • Page 12: Overview Of The Module

    Introduction Overview of the Module Overview of the Module The Fraction Collector transport mechanism uses a cartesian robot. The Y drive together with the Theta drive optimize the positioning for the fraction containers. The robot moves above a position and the diverter valve switches to collect in the according collection position.

  • Page 13: Fraction Collector Principle

    Introduction Fraction Collector Principle Fraction Collector Principle The movements of the Fraction Collector components during the sequence are monitored continuously by the Fraction Collector processor. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sequence is not completed successfully, an error message is generated.

  • Page 14: Leak And Waste Concept

    Introduction Leak and Waste Concept Leak and Waste Concept Rinse Port Drain Outlet 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 15: Leak Sensor

    Introduction Leak and Waste Concept Leak Sensor Leak sensor Leak tray Figure 2 Position of leak tray and leak sensor Solvent incompatibility C AU T I O N The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.

  • Page 16: Site Requirements And Specifications

    Site Requirements and Specifications Site Requirements Exhaust and Vapour Management Waste Management Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 17: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 22.
  • Page 18 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
  • Page 19 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 22) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.

  • Page 20: Exhaust And Vapour Management

    Site Requirements and Specifications Site Requirements Exhaust and Vapour Management You can operate the module in three different scenarios: 1 Under laboratory fume hood: • Ensure adequate fume hood ventilation: the recommended ventilation speed is 200 m /h with closed sash window and 490 m /h with open sash window.

  • Page 21: Waste Management

    Site Requirements and Specifications Site Requirements Waste Management Large amount of toxic, explosive, flammable, or corrosive solvents WAR N IN G Ensure that all waste is safely disposed according to your existing waste  management solution. For details, refer to the Site Preparation Checklist. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 22: Physical Specifications

    Site Requirements and Specifications Physical Specifications Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 30.6 kg (67.4 lbs) Dimensions 781 mm x 393 mm x 622 mm (height × width × depth) (30.7 inches, 15.5 inches, 24.5 inches) Line voltage 100 –...

  • Page 23: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Table 2 Performance Specifications Type Specification Comment Method/Conditions Delay volume Calculated by Lab Advisor. Dead volume in Valve tip appr. 30 µL Time to move from ves- 0.3 s Tested with OD 30 mm sel to vessel vessels.
  • Page 24 Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications Type Specification Comment Method/Conditions Peak-based with time slice recov- The complete output of the frac- tion collector is recovered into sep- arate slices of a specified duration. When a peak is discovered by a peak trigger, fractions are col- lected in dedicated vessels.
  • Page 25 Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications Type Specification Comment Method/Conditions Time-based, collecting volume Starting at a given time, the output slices of the fraction collector is col- lected into separate slices of a specified volume. Depending on the max.
  • Page 26 Site Requirements and Specifications Performance Specifications Table 2 Performance Specifications Type Specification Comment Method/Conditions Instrument Control LC & CE Drivers A.02.17 or above For details about supported soft- Instrument Control Framework ware versions refer to the compati- (ICF) A.02.04 or above bility matrix of your version of the Instant Pilot (G4208A) with firm- LC &...

  • Page 27: Using The Fraction Collector

    Using the Fraction Collector Warnings and Cautions Solvent Information Recommended Wash Solvents Material Information Turn on/off Status Indicators Drawer Status Indicator Exchange Drawers Exchange Containers Replace Inlet/Waste Tubings Configuration and Operation of the Open Bed Fraction Collector Delay Volumes and Delay Calibration Perfom a Delay Calibration Run in OpenLAB CDS Chemstation Edition Help: Delay Evaluation...

  • Page 28: Warnings And Cautions

    Using the Fraction Collector Warnings and Cautions Warnings and Cautions Leaking solvents WAR N IN G Ensure a safe drain of liquids into the inlet of your waste management  system. Regularily check all tubing connections for tightness and proper tubing ...

  • Page 29: Solvent Information

    Using the Fraction Collector Solvent Information Solvent Information Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety ...

  • Page 30: Recommended Wash Solvents

    Using the Fraction Collector Solvent Information Solvents Excluded from Intended Use The module is not designed for normal phase chromatography. Do not use solvents with an auto-ignition temperature below 200 °C (392 °F). Do not use solvents with a boiling point below 56 °C (133 °F). Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for example, carbon disulfide).

  • Page 31: Material Information

    Using the Fraction Collector Solvent Information Material Information Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
  • Page 32 Using the Fraction Collector Solvent Information When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions, normal PEEK capillaries are very sensitive to high pressure. Therefore, Agilent uses stainless steel cladded PEEK capillaries in bio-inert systems.
  • Page 33 Using the Fraction Collector Solvent Information Stainless Steel (SST) Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3.
  • Page 34 Using the Fraction Collector Solvent Information Titanium (Ti) Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and hypochlorous acid) over a wide range of concentrations and temperatures. This is due to a thin oxide layer on the surface, which is stabilized by oxidizing compounds.
  • Page 35 Using the Fraction Collector Solvent Information Platinum/Iridium Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents.

  • Page 36: Turn On/Off

    Using the Fraction Collector Turn on/off Turn on/off Plug in the power cable. Power switch: On Power switch: Off Unplug the power cable. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 37: Status Indicators

    Using the Fraction Collector Status Indicators Status Indicators The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed. 4. Error mode - interrupts the analysis and requires attention (for example a leak or defective internal components). 5.

  • Page 38: Drawer Status Indicator

    Using the Fraction Collector Drawer Status Indicator Drawer Status Indicator The drawer status indicator indicates one of three possible conditions: 1: off: drawer not detected 2: on: drawer detected and not in use = drawer can be pulled out 3: blinking: drawer detected and in use = drawer blocked NOTE Do not try to remove the drawer when the drawer status indicator is blinking.

  • Page 39: Exchange Drawers

    Using the Fraction Collector Exchange Drawers Exchange Drawers Parts required Description G9321-60085 Drawer ambient G9321-60201 InfinityLab Drawer Gilson 207, ambient Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks.
  • Page 40 Using the Fraction Collector Exchange Drawers Remove the drawer. Release the drawer by pushing the metal plate under- neath the drawer with the second hand. Exchange the drawer Install the drawer. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 41: Exchange Containers

    Using the Fraction Collector Exchange Containers Exchange Containers Parts required Description G9321-60015 Tube Container for 30 mm x 150 mm tubes, ambient, 10 tubes G9321-60058 Tube Container for 30 mm x 100 mm tubes, ambient, 10 tubes G9321-60025 Tube Container for 25 mm x 150 mm tubes, ambient, 18 tubes G9321-60035 Tube Container for 25 mm x 100 mm tubes, ambient, 18 tubes G9321-60129...
  • Page 42 Using the Fraction Collector Exchange Containers Insert the new container. NOTE Mind the correct orientation of the container on the drawer. Check if the container is fixed properly ("Click"). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 43: Replace Inlet/Waste Tubings

    Using the Fraction Collector Replace Inlet/Waste Tubings Replace Inlet/Waste Tubings Parts required Description G9321-60951 1290 Inf II PrepFC Tubing Kit 200 for flow rates up to 200 mL/min G9321-60952 1290 Inf II PrepFC Tubing Kit 50 for flow rates up to 50 mL/min G9321-60953 1290 Inf II PrepFC Tubing Kit 8 mL for flow rates up to 8 mL/min...
  • Page 44 Using the Fraction Collector Replace Inlet/Waste Tubings Remove the tubings from the tube holder. Remove tubing clip and dongle from the fraction collec- tor. Remove the Inlet/Waste Tubings from the capillary Remove the tubings from under the clamp at the robot- holder.
  • Page 45 Using the Fraction Collector Replace Inlet/Waste Tubings Screw the tubings of the new inlet/waste tubing kit into 10 Install the delay sensor holder. the valve assembly. Tighten the fittings finger-tight, then NOTE use the Fitting handle (about ¼ turn). Make sure that the holder is fixed properly. Press the small button to insert the tubing properly.
  • Page 46 Using the Fraction Collector Replace Inlet/Waste Tubings 15 Close the tube holder. 16 Install the Inlet/Waste Tubings to the capillary holder. Position them approximately in the middle of the capil- lary holder. 17 Install the top fume hood and close it. Push the top fume hood into the recession.

  • Page 47: Configuration And Operation Of The Open Bed Fraction Collector

    Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Delay Volumes and Delay Calibration Once software is installed and the fraction collector is ready to be operated, the fraction delay time needs to be determined.
  • Page 48 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Figure 4 Chromatogram from a UV-detector with peak starting at t and ending at t Performing a Delay Calibration with an UV Detector 1 Place a vial containing the Delay Sensor Calibrant (5190-8223) in position 1 of the autosampler.

  • Page 49: Perfom A Delay Calibration Run In Openlab Cds Chemstation Edition

    Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Perfom a Delay Calibration Run in OpenLAB CDS Chemstation Edition The delay calibration procedure determines the delay time between detector(s) and the fraction collector in the system. The delay is used to compensate for the time a compound needs to travel between the point of detection in the detector and the point of collection in the fraction collector.

  • Page 50: Help

    Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Help: Start The Start page of the Delay Calibration Wizard contains a description of the fraction collector delay calibration process, together with a schematic diagram of the connections for the detector and fraction collector. Cancel Closes the Delay Calibration Wizard without consequence.
  • Page 51 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Prepare Instrument for Calibration The Prepare Instrument page of the Delay Calibration Wizard leads you through the preparation of the instrument for the delay calibration run. The required preparation steps are listed in a three-column table: Activity A short description of the preparation activity.
  • Page 52 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Perform Calibration Run Use the Perform Calibration page of the Delay Calibration Wizard to start the delay calibration run using the current method. The run parameters are listed; a warning is given if the method has been changed.

  • Page 53: Delay Evaluation

    Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Delay Evaluation The Delay Evaluation window enables you to determine the delay times/volumes between the peak detector(s) and the fraction collector. The Delay Evaluation window is split into two sections: •...
  • Page 54 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Delay Calibration The Delay Calibration pane contains the parameters and results of the delay calculations: Pump Flow The pump flow as given in the data file or as a user-specified value. The flow specified here is used to calculate the delay volumes.
  • Page 55 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Signals The Signals pane contains a signal plot for each peak detector, and one for the fraction collector delay sensor. By default, the largest peak in each signal plot is identified and highlighted as the target peak, but you can change the identification if there is more than one peak in the plot and the wrong peak has been identified.

  • Page 56: Setting Up A Fraction Collector Method

    Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Setting up a Fraction Collector Method Fraction Trigger Mode Use Timetable: Enables the Timetable, but requires a timetable event. Peak-based: If Peak-based is selected, the collection of a fraction is triggered by the signal of the detector.
  • Page 57 Peak Detectors In the Peak Detectors section a list of all peak detectors that are connected to the system is displayed. Agilent InfinityLab LC Series diode array detectors, multiwavelength detectors, variable wavelength detectors and fluorescence detectors are recognized automatically. Other detectors, e.g. Agilent 6000 mass-selective detectors or HP1050 detectors, are connected through the Universal Interface Box (UIB).
  • Page 58 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector peak detector detects a peak basing on the settings in the peak detectors table above. If an MSD is used for mass-based fraction collection, Use MSD for mass-based Fraction Collection must be checked.
  • Page 59 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector Auxiliary In the Auxiliary section the Maximum fill volume per location can be specified. If as configured is selected, the pre-configured volume is used. This ensures that the location (well, vial or tube) cannot be overfilled during fraction collection.
  • Page 60 Using the Fraction Collector Configuration and Operation of the Open Bed Fraction Collector The Fraction Preview screen allows to test the fraction collection parameters against an example chromatogram. It can also be used to optimize the fraction collection parameters interactively. With the help of this tool values for up and down slope as well as for upper and lower threshold can easily be graphically specified.

  • Page 61: Prepare The Module For Transportation

    Using the Fraction Collector Prepare the Module for Transportation Prepare the Module for Transportation When • For transportation within the laboratory • For shipping the module Parts required Description Transport Foam Shipping Block Screw M3 Protect Foam Y-Axis Preparations • Remove all containers and drawers.
  • Page 62 Using the Fraction Collector Prepare the Module for Transportation Using "Single Steps" from the Service and Diagnostics Insert the shipping block into the rail on the collector section in Lab Advisor, move the robotics to the park arm. position. 120° Move the shipping block backward on the rail until the Fix the shipping bolt with the M3 screw.

  • Page 63: Preparing The Fraction Collector

    Preparing the Fraction Collector Best Practices Regular Inspections Power up / Shut down Prepare the Fraction Collector Using the Fraction Collector Capillary Color Coding Guide Swage Fittings Setting up the Fraction Collector with the Instrument Control Interface Overview Instrument Configuration Fraction Collector User Interface (Dashboard Panel) Method Parameter Settings Advanced Settings...

  • Page 64: Best Practices

    Preparing the Fraction Collector Best Practices Best Practices Regular Inspections Inspect the inlet/waste tubings and exchange them if they are worn out or show visible signs of damage. Power up / Shut down Power up • Check that the robotics is not obstructed. Shut down •...

  • Page 65: Capillary Color Coding Guide

    Preparing the Fraction Collector Capillary Color Coding Guide Capillary Color Coding Guide Figure 7 Syntax for capillary description 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 66: Swage Fittings

    Preparing the Fraction Collector Swage Fittings Swage Fittings Select a nut that is long enough for the fitting you'll be Slide the nut over the end of the tubing or capillary. using. Carefully slide the ferrule components on after the nut Use a column or injection valve to gently tighten the fit- and then finger-tighten the assembly while ensuring ting which forces the ferrule to seat onto the tubing or...
  • Page 67 Preparing the Fraction Collector Swage Fittings Loosen the nut and verify that the ferrule is correctly positioned on the tubing or capillary. The first time that the swagelock fitting is used on a column or an injection valve, NOTE the position of the ferrule is permanently set. If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening.

  • Page 68: Setting Up The Fraction Collector With The Instrument Control Interface

    Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Setting up the Fraction Collector with the Instrument Control Interface Overview Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software.
  • Page 69 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Figure 8 Instrument Configuration 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 70 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface The Fraction Collector configuration parameters are in four sections: • Communication • Module List • Peak Detectors • Linked Pump Table 3 Instrument configuration parameters Parameter Description Communication: The parameters in this dialog box are detected automatically during autoconfigura-...
  • Page 71 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Table 3 Instrument configuration parameters Parameter Description Peak Detectors: • Module type: product number of the peak detector detected during autoconfiguration • Serial number: serial number of the peak detector detected during autoconfiguration •...

  • Page 72: Fraction Collector User Interface (Dashboard Panel)

    Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Fraction Collector User Interface (Dashboard Panel) Table 4 Fraction Valve User Interface Parameter Description Module graphic The items in the Fraction Collector graphic have the following meaning and function: •...
  • Page 73 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Table 4 Fraction Valve User Interface Parameter Description Context Menu The context menu of the dashboard panel contains the following commands: • Method: Displays the Fraction Collector's Method Setup dialog box.

  • Page 74: Method Parameter Settings

    Preparing the Fraction Collector Method Parameter Settings Method Parameter Settings Figure 9 Method settings 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 75 Preparing the Fraction Collector Method Parameter Settings The Fraction Collector method setup parameters are in eight sections: • Collection Behavior • Peak Triggers • Trigger Combinations • Stoptime • Posttime • Advanced • Timetable • Fraction Preview Table 5 Method Parameter Settings Parameter Description Collection Behavior...
  • Page 76 Preparing the Fraction Collector Method Parameter Settings Table 5 Method Parameter Settings Parameter Description Trigger Combinations Use the Trigger Combinations to specify how multi- ple peak triggers are combined to start or stop Fraction Collection. You can choose that: • Collection of a fraction is started when all peak detectors have sent a start trigger, and contin- ues until one detector sends a stop trigger (AND...

  • Page 77: Advanced Settings

    Preparing the Fraction Collector Method Parameter Settings Advanced Settings Figure 10 Advanced settings The Fraction Collector method setup advanced parameters are in three sections, depending on the configuration: • Delay Settings • Fill Volume Settings • 3rd Party Pump Flow (only visible if there is no Agilent pump recognized.) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 78 Preparing the Fraction Collector Method Parameter Settings Table 6 Advanced Parameters Description Parameter Description Delay Settings Use the Delay Settings table to specify the delay that is applied to a peak trigger signal. You can specify this setting for each peak detector sepa- rately.

  • Page 79: Timetable Settings

    Preparing the Fraction Collector Method Parameter Settings Timetable Settings A timetable entry is crucial to enable any fraction collection. NOTE Figure 11 Timetable settings Use the Timetable to program changes in the fraction collector parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable.
  • Page 80 Preparing the Fraction Collector Method Parameter Settings Table 7 Timetable Functions Function Parameters Fraction Mode • Off (Turns off the current fraction collection, where you use Off to turn off fraction collection at the end of the run if you have not specified a Stoptime) •...
  • Page 81 Preparing the Fraction Collector Method Parameter Settings Table 7 Timetable Functions Function Parameters Trigger Settings • Trigger Source (Click the down-arrow and select the trigger source from the drop-down list) • Peak Detection Mode (Click the down-arrow and select the peak detection mode from the drop-down list).

  • Page 82: Pooling

    Preparing the Fraction Collector Pooling Pooling Pooling is the collection of multiple fractions into the same collection vessel. You can pool fractions from either multiple injections of the same sample or single injections of different samples. Fractions are pooled automatically when you specify multiple injections from the same location in one line of the sequence table, or if the same fraction start location is specified for sequential locations in the sequence table.

  • Page 83: Troubleshooting And Diagnostics

    Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 84: User Interfaces

    Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • Preferred tool should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 85. • The Agilent OpenLAB ChemStation C.01.03 and above do not include any maintenance/test functions.

  • Page 85: Agilent Lab Advisor Software

    Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.

  • Page 86: Error Information

    Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
  • Page 87 Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

  • Page 88: Timeout

    Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and source The analysis was completed successfully, of a not-ready condition.

  • Page 89: Shutdown

    Error Information General Error Messages Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.

  • Page 90: Remote Timeout

    Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.

  • Page 91: Leak Sensor Short

    Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.

  • Page 92: Leak Sensor Open

    Error Information General Error Messages Leak Sensor Open Error ID: 0083 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits.

  • Page 93: Compensation Sensor Short

    Error Information General Error Messages Compensation Sensor Short Error ID: 0080 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.

  • Page 94: Leak

    Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.

  • Page 95: Maintenance

    Maintenance Introduction to Maintenance Warnings and Cautions Cleaning the Module Overview of Maintenance Install and Remove the Top Fume Hood Clean the Leak Pan Replace the Dripping Adapter for the Fraction Valve Replace the Module Firmware This chapter describes the maintenance of the module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 96: Introduction To Maintenance

    Maintenance Introduction to Maintenance Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system. There are no serviceable parts inside. NOTE Do not open the rear of the module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 97: Warnings And Cautions

    Maintenance Warnings and Cautions Warnings and Cautions Module is partially energized when switched off, as long as the power cord WAR N IN G is plugged in. Risk of stroke and other personal injury. Repair work at the module can lead to personal injuries, e.
  • Page 98 Maintenance Warnings and Cautions Electronic boards and components are sensitive to electrostatic discharge C AU T I O N (ESD). ESD can damage electronic boards and components. Be sure to hold the board by the edges, and do not touch the electrical ...

  • Page 99: Cleaning The Module

    Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Liquid dripping into the electronic compartment of your module can cause WAR N IN G shock hazard and damage the module Do not use an excessively damp cloth during cleaning.

  • Page 100: Overview Of Maintenance

    Maintenance Overview of Maintenance Overview of Maintenance The procedures described in this section can be done with the Fraction Collector in place in the stack. These procedures can be done on a more frequent basis. Table 8 Overview of maintenance procedures Procedure Typical Frequency Notes...

  • Page 101: Install And Remove The Top Fume Hood

    Maintenance Install and Remove the Top Fume Hood Install and Remove the Top Fume Hood Parts required Description G9321-68200 Top Fume Hood Kit See chapter Parts for details. Install the exhaust sheet by fixing the four screws to the Install the top fume hood and close it. fraction collector.
  • Page 102 Maintenance Install and Remove the Top Fume Hood Open and remove the top fume hood. Remove the screws fixing the exhaust sheet to the frac- tion collector, then remove the exhaust sheet. Swing up the fume hood against the mechanical stop.

  • Page 103: Clean The Leak Pan

    Maintenance Clean the Leak Pan Clean the Leak Pan Preparations • Turn off the flow and the instrument. • Remove all drawers from the module. • Move the robotics to the home position. Pull the release knobs on both sides at the front of the Open the guide support assembly (1.) and fix it at the guide support assembly.

  • Page 104: Replace The Dripping Adapter For The Fraction Valve

    Maintenance Replace the Dripping Adapter for the Fraction Valve Replace the Dripping Adapter for the Fraction Valve Tools required Description 5067-7028 Z-Axe Maintenance Tool This tool has a bifunctional shank. One side serves as a tool for exchanging the needle tubing. The other side is designed for removing and installing of the dripping adapter.
  • Page 105 Maintenance Replace the Dripping Adapter for the Fraction Valve Remove Z-Axe Maintenance Tool (5067-7028). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 106: Replace The Module Firmware

    Maintenance Replace the Module Firmware Replace the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...

  • Page 107: Parts For Maintenance And Repair

    Parts for Maintenance and Repair Supported Containers List of Recommended Fraction Tubes Fraction Collector Accessory Kit Tubing Kits Top Fume Hood Kit This chapter provides information on parts for maintenance and repair. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 108: Supported Containers

    Parts for Maintenance and Repair Supported Containers Supported Containers Description G9321-60015 Tube Container for 30 mm x 150 mm tubes, ambient, 10 tubes G9321-60058 Tube Container for 30 mm x 100 mm tubes, ambient, 10 tubes G9321-60025 Tube Container for 25 mm x 150 mm tubes, ambient, 18 tubes G9321-60035 Tube Container for 25 mm x 100 mm tubes, ambient, 18 tubes G9321-60129...

  • Page 109: List Of Recommended Fraction Tubes

    Parts for Maintenance and Repair List of Recommended Fraction Tubes List of Recommended Fraction Tubes Description 5190-9090 Fraction Tubes 150 mm x 30 mm, 78 mL (pack of 100) fits with p/n G9321-60015 5042-6458 Fraction Tubes 100 mm x 30 mm, 50 mL (pack of 100) fits with p/n G9321-60058 5190-9091 Fraction Tubes 150 mm x 25 mm, 55 mL (pack of 100)

  • Page 110: Fraction Collector Accessory Kit

    Parts for Maintenance and Repair Fraction Collector Accessory Kit Fraction Collector Accessory Kit The Fraction Collector Accessory Kit (G9321-68002) contains the following items: Description 5065-9971 Barbed Y-Connector, PP for 3/16 in ID Tube 5181-1519 CAN cable, Agilent module to module, 1 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5063-6527...

  • Page 111: Tubing Kits

    Parts for Maintenance and Repair Tubing Kits Tubing Kits Tubing Kit G9321-60951 1290 Inf II PrepFC Tubing Kit 200 (G9321-60951) contains the following parts: Description G9321-40081 Delay Sensor Clip G9321-40128 Tube holder 5023-2878 2.4 m Tubing ESD PTFE (OD2/ID1.2) 5023-2636 2.4 m Tubing ESD PTFE (OD2.5/OD1.6) 5023-2871 Fitting 1/4-28 for Tube-OD 2.5 mm ESD-PEEK...
  • Page 112 Parts for Maintenance and Repair Tubing Kits Tubing Kit G9321-60952 1290 Inf II PrepFC Tubing Kit 50 (G9321-60952) contains the following parts: Description G9321-40081 Delay Sensor Clip G9321-40128 Tube holder 5067-6785 EEPROM tag assembly 5023-2637 2.4 m Tubing ESD PTFE (OD1.6/ID0.8) 5023-2636 2.4 m Tubing ESD PTFE (OD2.5/OD1.6) 5023-2871...
  • Page 113 Parts for Maintenance and Repair Tubing Kits Tubing Kit G9321-60953 1290 Inf II PrepFC Tubing Kit 8 mL (G9321-60953) contains the following parts: Description G9321-40081 Delay Sensor Clip G9321-40128 Tube holder 5067-6785 EEPROM tag assembly 5023-3072 2.4 m Tubing ESD PTFE (OD1.6/ID0.5) 5023-2636 2.4 m Tubing ESD PTFE (OD2.5/OD1.6) 5023-2874...
  • Page 114 Parts for Maintenance and Repair Tubing Kits Tubing Kit G9321-60954 1290 Inf II PrepFC Tubing Kit 150 mL (G9321-60954) contains the following parts: Description G9321-40081 Delay Sensor Clip G9321-40128 Tube holder 5067-6785 EEPROM tag assembly 5023-2878 2.4 m Tubing ESD PTFE (OD2/ID1.2) 5023-2636 2.4 m Tubing ESD PTFE (OD2.5/OD1.6) G9321-87713...

  • Page 115: Top Fume Hood Kit

    Parts for Maintenance and Repair Top Fume Hood Kit Top Fume Hood Kit Item Description G9321-68200 Top Fume Hood Kit contains the following items: G9321-60830 Top Fume Hood 0515-1753 SCREW-MACH-M3X0.5-8MM-LG-PAN-HD G9321-00100 Exhaust Sheet G1364-03201 Exhaust Tube Adapter 5067-5406 Screw Tx20, M4 x 8 mm Figure 12 Top Fume Hood Kit 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 116: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 117: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
  • Page 118 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion) RS-232 cables (not for Description FUSION board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).

  • Page 119: Analog Cables

    Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
  • Page 120 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...

  • Page 121: Remote Cables

    Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced...
  • Page 122 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding...
  • Page 123 High Ground Cable Shield- One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 124 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...

  • Page 125: Can/Lan Cables

    Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...

  • Page 126: Cables

    Identifying Cables RS-232 Cables RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

  • Page 127: Usb

    Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 128: Hardware Information

    Hardware Information Firmware Description Electrical Connections Serial Number Information (ALL) Side View of the Module Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Early Maintenance Feedback Instrument Layout This chapter describes the module in more detail on hardware and electronics. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 129: Firmware Description

    Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
  • Page 130 Hardware Information Firmware Description Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used). Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761 The file naming conventions are: PPPP_RVVV_XXX.dlb, where •...
  • Page 131 Hardware Information Firmware Description Some modules are limited in downgrading due to their main board version or NOTE their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments.

  • Page 132: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.

  • Page 133: Serial Number Information (All)

    Hardware Information Electrical Connections Serial Number Information (ALL) The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambig- uously denotes a module (there can be more than one code for the same module)

  • Page 134: Interfaces

    Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 10 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers...
  • Page 135 Hardware Information Interfaces Table 10 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with addi-...

  • Page 136: Overview Interfaces

    Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 137 Hardware Information Interfaces To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the system’s critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules.

  • Page 138: Eri (Enhanced Remote Interface)

    ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal...
  • Page 139 Hardware Information Interfaces Enhanced Remote IO 1 (START REQUEST) IO 2 (STOP) IO 3 (READY) IO 4 (POWER ON) IO 5 (NOT USED) IO 6 (SHUT DOWN) IO 7 (START) IO 8 (PREPARE) 1 wire DATA DGND +5 V ERI out PGND PGND +24 V ERI out...

  • Page 140: Usb (Universal Serial Bus)

    Hardware Information Interfaces 5V Distribution (Future Use) • Available directly after turn on oft the hosting module (assures that certain base functionality of the device can be detected by firmware). • For digital circuits or similar. • Provided 500 mA maximum. •...

  • Page 141: Setting The 6-Bit Configuration Switch

    Hardware Information Setting the 6-bit Configuration Switch Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with FUSION electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures. All modules with FUSION electronics: •...
  • Page 142 Hardware Information Setting the 6-bit Configuration Switch Table 12 6-bit Configuration Switch Mode Function/Setting Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 n.a. LAN Init Mode n.a. n.a. Use Default IP Address Use Stored IP Address Use DHCP to request IP Address Test System...

  • Page 143: Special Settings

    Hardware Information Setting the 6-bit Configuration Switch Special Settings Boot-Resident/Main Firmware update procedures may require this mode in case of firmware loading errors (main/resident firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident/main mode.
  • Page 144 Hardware Information Setting the 6-bit Configuration Switch Table 13 Boot Resident / Forced Coldstart Init Mode Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential.

  • Page 145: Early Maintenance Feedback

    Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.

  • Page 146: Instrument Layout

    Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.

  • Page 147: Appendix

    In Case of Damage Solvents Safety Symbols Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides additional information on safety, legal, and web. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 148: General Safety Information

    Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.

  • Page 149: Before Applying Power

    Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 150: Do Not Operate In An Explosive Atmosphere

    Appendix General Safety Information Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WAR N IN G Explosion hazard Do not operate the instrument in the presence of flammable gases or  fumes. Do Not Remove the Instrument Cover Instrument covers removed WAR N IN G Electrical shock...

  • Page 151: Solvents

    Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety  procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

  • Page 152: Safety Symbols

    Appendix General Safety Information Safety Symbols Table 14 Symbols The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
  • Page 153 Appendix General Safety Information Table 14 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

  • Page 154: Waste Electrical And Electronic Equipment (Weee) Directive

    Appendix Waste Electrical and Electronic Equipment (WEEE) Directive Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see...

  • Page 155: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

  • Page 156: Sound Emission

    Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 157: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 158 • optimizing, • troubleshooting and diagnostics, • errors, • test functions, • maintenance, • parts and materials, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2016-2019 Published in Germany 11/2019 Document No: SD-29000163 Rev. F...

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