Data Analysis For Comprehensive 2D-Lc (Lcxlc) - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

8 Data Analysis

Data Analysis for Comprehensive 2D-LC (LCxLC)

Data Analysis for Comprehensive 2D-LC (LCxLC)

Typically very complex samples are analyzed by comprehensive 2-dimensional
liquid chromatography. The compounds which are often co-eluting form the first
dimension are further separated in the second dimension. With the Agilent 1290
Infinity II 2D-LC Solution always one large data-file spanning the run-time of the
two-dimensional analysis will be acquired. As an example, a 2-dimensional
analysis of a mixture of 26 polyphenolic standard compounds is shown in a one
dimensional data analysis display
data can be analyzed with OpenLAB CDS ChemStation edition software.
But for easier data-analysis and better visualization of the comprehensive 2D-LC
data special software is recommended. Agilent recommends GC Image LCxLC
edition Software from GC Image LLC, Nebraska, USA. A trial download can be
found on
files also including UV spectra and mass spectra data can be directly imported.
This software, with the information of the modulation time, is capable to extract
the data and isolate each second dimension run. Data will be reconstructed in a
two-dimensional display of the retention times. This can be displayed as a
colored 2-dimensional map of compound peaks
baseline correction the peaks can be automatically detected by a peak detection
algorithm inherent in the 2D-LC data analysis software
Since the third dimension is the intensity of the peaks a 3-dimensional plot of the
data is possible (
qualitative and quantitative data analysis is possible.
2D-LC - User Guide
www.GCImage.com as well as an online manual. Agilent 2D-LC data
Figure 97 on page 221). With the given data set further
(Figure 94 on page 220). Theoretically, the
(Figure 95
(Figure 96
on page 220). After
on page 221).
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