Comprehensive 2D-Lc And Active Solvent Modulation - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

2 Concepts of 2D-LC
Active solvent Modulation (ASM)
Comprehensive 2D-LC and Active Solvent
Modulation
The ASM Valve can also be used for improving comprehensive 2D-LC
measurements, but it is primarily optimized for multiple heart-cutting and
high-resolution sampling measurements.
The ASM phase contributes to the modulation cycle. When keeping the
modulation time constant, this reduces available time for the separation phase of
the cycle. Otherwise, increasing the modulation time may require reducing the
flow rate to fill the same sample loop volume. This would change
chromatography.
The ASM solution requires back pressure from capillaries between the 2D-LC
valve to multiple heart-cutting valves. Therefore, comprehensive 2D-LC sample
loops cannot be installed directly at the ASM valve. In addition, comprehensive
2D-LC sample loops have standard fittings, which do not fit to the M4 ports of the
ASM valve.
Please note that ASM valves require twice as many switches as a standard
2D-LC valve. Comprehensive 2D-LC uses many valve switches and in
combination with ASM, this may reduce the maintenance interval of the valve.
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