Checkout/Familiarization Procedure - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

6 Run the System
Comprehensive 2D-LC

Checkout/Familiarization Procedure

Checkout run - comprehensive (standard setup)
The familiarization procedure illustrates the system´s 2D-LC capabilities and
supports the user to start the method for a specific analytical task. The
familarization procedure will guide the user through the most important setups
and analysis function, described in the chapters before.
The sample provided with the familiarization procedure can be determined with a
UV-detector and a mass spectrometer. The methods to analyze the starter
sample are delivered together with the full package to ensure a smooth
familarization and checkout procedure. With the given method, peaks will overlap
in the first dimension and will be separated in the second dimension.
The Agilent 1290 Infinity II 2D-LC Solution is delivered together with all required
parts for a complete familiarization procedure for (multiple) heart-cutting and
comprehensive 2D-LC.
Parts required p/n
5190-6895
858700-902
959757-302
G2453-85060
Software required
CD
Preparations
Solvents needed:
•
•
Preparations:
1
2
3
2D-LC - User Guide
Description
2D-LC starter sample, 1 x 2 mL
RRHD SB-C18, 2.1x100 mm, 1.8 µm, 1200 bar
1
D
RRHD Eclipse Plus C18, 3.0x50 mm, 1.8 µm
Formic Acid-Reagent Grade 5 mL (5 cc)
1D: mobile phase A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060), B
= methanol
2D: mobile phase A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060), B
= acetonitrile
Prepare dilution solvent (20 % MeOH in mobile phase A): Add 500 µL MeOH to 2000 µL Mobile
Phase A.
Prepare 400 µL sample: Add 40 µL 2D LC starter sample to 360 µL dilution solvent.
Load method Checkout_MHC_FullComp_1290BinX1290Bin.MCheckout_MHC_FullCom-
p_1290QuatX1290Bin.M, or Checkout_MHC_FullComp_1260BinX1290Bin.M(depends on your
pump setup) from the CD.
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