End Cycle; Gradient Step - Agilent Technologies SureCycler 8800 Setup And User Manual

The fields in the Increments per Cycle section are used to set temperature and time
N O T E
increments for the temperature step during cycling. The use of increments is an optional
feature. Leave these fields blank if you do not want to add increments.
Temperature
Enter the desired increase or decrease in the processing temperature with
Increment
each cycle. Press the +/– button to toggle between positive and negative
numbers. Negative numbers (e.g. –2°C) indicate an incremental decrease in
temperature with each cycle.
Time Increment:
Enter the desired increase in the Hold For time with each cycle. The units
for this field are seconds/cycle.

End Cycle

Insert this program step to terminate a PCR cycle. Put this step after a
series of steps that are to be repeated for several cycles.
To have a valid program, the steps of a cycle need to be contained between a Start Cycle
N O T E
and an End Cycle command.

Gradient Step

A Gradient Step is similar to a Temperature Step, but in a Gradient Step
the thermal block holds a temperature gradient (rather than a single
temperature across the entire block) for a specified length of time.
The Gradient Step is useful for optimizing the annealing temperature for
your particular primer/template system.
Agilent SureCycler 8800 Setup and User's Guide
PCR Programs 4
End Cycle
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