Investigate The Effects Of Using Different Gradients In The 2Dimension - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

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Investigate the effects of using different gradients in the 2Dimension

Investigate the effects of using different gradients in the 2Dimension
Investigate the effects of using different
gradients in the
When combining separation systems with related separation mechanisms in the
first and second dimension (as in RPxRP), orthogonality is limited. As a result,
only a part of the available two-dimensional separation space will be occupied. In
such a case, shifted gradients in the second dimension can be used to enlarge
the accessible two-dimensional separation space.
1 To investigate the effects of using different gradients in the second
2D-LC - User Guide
dimension, firstly run a comprehensive 2D-LC separation with the same
second dimension gradient from 5-95 % B repeated during the whole run.
The 1D pump method should be set up as during the checkout runs (see
below):
2
Dimension
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