Set 2D-Lc Mode Hires Sampling - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC

Set 2D-LC Mode HiRes Sampling

Setting the mode has the following consequences (for details, see
Resolution Sampling - Peak Parking Principles"
•
Considerations for high-resolution sampling 2D-LC
NOTE
In high-resolution sampling 2D-LC keep the following general considerations in
mind, when setting up the experiments (see
Parking Principles"
1 Each loop for consecutive snips stores the same sample volume.
2 First and last loop cannot be used for parking.
3 Solvent transfer from
4 Cut number 5 cannot be parked entirely in the sample loop. Otherwise cut 6
5 For parking cut 6 in the sample loop, the cut first needs to be moved from the
1 Select the HiRes sampling Mode.
2D-LC - User Guide
HiRes sampling:
In contrast to Heart-Cutting, which uses the continuous flow-through principle,
the MHC valve is switched before and after parking the peak.
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would got partially to the transfer capillary and would therefore be lost or spoil
cut 5.
Cut 5 stays partially in the transfer line and is immediately being analyzed in
2
D.
2D-LC valve to the deck valve. This new volume must be defined in the
configuration of the 2D-LC system.
"High Resolution Sampling - Peak
1 2
D to D can be reduced.
"High
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