Theoretical Basis Of 2D-Lc - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

15 Theoretical Background

Theoretical basis of 2D-LC

Theoretical basis of 2D-LC

In 2D-LC, fractions from a chromatografic system (1
to a second chromatographic separation system (2
bases on the application of two independent liquid phase separation systems to
a sample. 2D-LC is mainly used to improve resolution and sensitivity or to
decrease analysis time.
Figure 119 Peak capacity relationship between peak capacities of orthogonal first and second
2D-LC - User Guide
dimension
st
dimension) are transferred
nd
dimension). So 2D-LC
Peaks not resolved in first
dimension separation
Peaks resolved in second
dimension separation
323