Set 2D-Lc Mode - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Comprehensive 2D-LC

Set 2D-LC Mode

Setting the mode has the following consequences:
•
1 Select Comprehensive in 2D-LC Mode.
The Modulation time reflects the duration of one injection cycle in the 2
NOTE
dimension. After that time, the solvent composition gradient will be repeated.
The parameter Modulation time is only used in the Comprehensive mode.
The
dimension; the smallest value is 0.01 min. After that time, the Percent B value
before the gradient (or the timetable entry at time = 0.0) is restored. In the
Comprehensive 2D-LC mode the gradient stops latest when the Modulation time
is reached.
2D-LC - User Guide
Comprehensive 2D-LC:
The entire volume of the 1
nd
2 dimension) onto the 2
while one loops is filled in the 1st dimension, the volume of the other loop is
nd
separated with the 2
2
D Gradient Stoptime reflects the maximal duration of the gradient in the 2
Figure 62 2D-LC Comprehensive mode
nd
The gradient of the 2
lower right part of the method screen showing also the Stop time (as a red
vertical line) and the Modulation time as a green vertical line.
Figure 63 Stop time and Modulation time
st
dimension will be injected (using the pump in the
nd
column. Two identical loops are used alternating,
column.
dimension is graphically displayed in a window in the
nd
nd
128