Data Analysis For Heartcutting 2D-Lc (Lc-Lc); Data Analysis - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

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Data Analysis

Data Analysis for Heartcutting 2D-LC (LC-LC)

Data Analysis for Heartcutting 2D-LC (LC-LC)

For data-analysis of heart-cutting 2D-LC data OpenLAB CDS ChemStation edition
is usually fully sufficient.
Again, the data will be stored in one data-file. If more than one peak was analyzed
in the second dimension they will simply follow one after the other in a distance
of the second dimension run-time.
If a detector right after the first dimension column was used, e.g. as peak
detector for peak triggered operation, these data will be available as a second
data-trace.
st
1 dimension separation
Impurity
nd
dimension separation
2
Figure 82
2D-LC - User Guide
Main compound -
Impurity
Example of a heartcutting 2D-LC experiment. Data analysis and display done by
OpenLAB CDS ChemStation Edition, the data of a detector placed after the first
dimension column (blue) and of the 2D data detector (green). The additional impurities
(marked red) could not be detected by 1D-LC only.
pure?
Impurity
Main compound
Additional impurities!
201