Multiple Heart-Cutting And High Resolution Sampling 2D-Lc - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

2 Concepts of 2D-LC

Multiple Heart-Cutting and High Resolution Sampling 2D-LC

Multiple Heart-Cutting and High Resolution
Sampling 2D-LC
Typically, the gradient time in the second dimension is much longer for
heart-cutting than with the comprehensive technique. The disadvantage of the
standard heart-cutting techniques is that peaks cannot be sampled while a
second dimension gradient is still running. In the examples shown here, the
gradient from the second dimension is analyzing the first peak (purple), while the
second and third peak (gray and yellow) elute from the first dimension column.
The second dimension is ready when the 4th peak (green) elutes from the first
dimension; this peak can be analyzed. As the second dimension is occupied
again, the fifth peak (blue) cannot be analyzed.
2D-LC - User Guide 17