Set Solvent Composition Gradient - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

5 Method Parameters
Method Parameters Multiple Heart-Cutting and High Resolution Sampling
2D-LC

Set Solvent Composition Gradient

Set Solvent Composition Gradient
The timetable in the
Percent B ranges from 0 – 100 %.
Change the solvent composition at a specified time
1 To change the solvent composition (%B) at the specified time apply a percent
Different start conditions in the first row may cause step gradients and RI-effects
NOTE
(density differences of the different liquid phases may cause different DAD
detection through baseline disturbances).
2D-LC - User Guide
2
D Gradient group allows changing the solvent composition.
B range from 0 – 100 %
The time axis relates to the Stoptime of the 2
0.00 marks the start of the maybe repetitive gradient cycles, a time greater
2
than Stoptime D will be ignored.
nd
dimension pump. Time [min] =
111