Agilent Technologies InfinityLab LC Series User Manual
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Agilent Technologies InfinityLab LC Series User Manual

Agilent Technologies InfinityLab LC Series User Manual

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1260 Infinity II SFC System
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  • Page 1 Agilent InfinityLab LC Series 1260 Infinity II SFC System User Manual Agilent Technologies...
  • Page 2 Notices © Agilent Technologies, Inc. 2010 - 2016, Warranty receive no greater than Restricted Rights as 2017 defined in FAR 52.227-19(c)(1-2) (June The material contained in this docu- 1987). U.S. Government users will receive No part of this manual may be reproduced ment is provided “as is,”...
  • Page 3 In This Guide... In This Guide... This manual covers the Agilent InfinityLab LC Series 1260 Infinity II SFC System. 1 Introduction to Supercritical Fluid Chromatography (SFC) This chapter provides an overview of the history, theory and benefits of SFC. 2 Site Requirements and Specifications...
  • Page 4 In This Guide... 8 Identifying Cables This chapter provides information on cables used with the modules. 9 Appendix This chapter provides addition information on safety, legal and web.

  • Page 5: Table Of Contents

    Contents Contents 1 Introduction to Supercritical Fluid Chromatography (SFC) History of SFC Theory of SFC Benefits of SFC Common flow path overview for packed column SFC instrumentation The Agilent 1260 Infinity II SFC System 2 Site Requirements and Specifications Site Requirements Specifications 3 Installing the Agilent 1260 Infinity II SFC System Hardware Installation...
  • Page 6 Agilent 1260 Infinity II SFC DAD Parts 8 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables 9 Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet...

  • Page 7: Introduction To Supercritical Fluid Chromatography (Sfc)

    The Agilent 1260 Infinity II Multicolumn Thermostat (G7116A) The Agilent 1260 Infinity II Diode Array Detector WR (G7115A) and Agilent 1260 Infinity II Multi Wavelength Detector (G7165A) Applications Columns This chapter provides an overview of the history, theory and benefits of SFC. Agilent Technologies...

  • Page 8: History Of Sfc

    Introduction to Supercritical Fluid Chromatography (SFC) History of SFC History of SFC Supercritical fluid chromatography (SFC) was first introduced by Klesper et al. in 1962 (Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962, 27,700.) for the separation and analysis of a porphyrin mixture using open tubular SFC.

  • Page 9: Theory Of Sfc

    Introduction to Supercritical Fluid Chromatography (SFC) Theory of SFC Theory of SFC Figure 1 State of a solvent The superior separation properties achieved by SFC can be explained best by the thermodynamics of liquids and gases (see the phase diagram in Figure 1 on page 9).

  • Page 10: Benefits Of Sfc

    Introduction to Supercritical Fluid Chromatography (SFC) Benefits of SFC Benefits of SFC SFC is widely accepted for the analysis and separation of chiral compounds. In addition, it gains increasing acceptance as a complementary liquid-based separation technique to HPLC for high-throughput and high-resolution analysis of complex mixtures.

  • Page 11: Common Flow Path Overview For Packed Column Sfc Instrumentation

    Introduction to Supercritical Fluid Chromatography (SFC) Common flow path overview for packed column SFC instrumentation Common flow path overview for packed column SFC instrumentation In commercially available SFC systems, CO is initially pumped in liquid state and is brought into the supercritical state by heating it above the critical temperature before it enters the high-pressure area of the LC instrument.

  • Page 12: The Agilent 1260 Infinity Ii Sfc System

    Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II SFC System consists of a binary SFC pump (G4782A), an SFC Multisampler (4767A), a thermostatted column compartment (G7116A), and either a DAD (G7115A) or MWD (G7165A) equipped with a high pressure SFC flow cell and the SFC control module (G4301A).
  • Page 13 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II SFC Control Module (G4301A) The Agilent 1260 Infinity II SFC Control Module is responsible for all tasks connected to pre- and post-conditioning of the mobile phase. In contrast, flow rate, mobile phase composition, detection, column temperature and data analysis are controlled by the modules of the Agilent 1260 Infinity II SFC system in combination with the ChemStation software.
  • Page 14 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II SFC Binary Pump (G4782A) The 1260 Infinity II SFC Binary pump is equipped with passive inlet valves and with special seals and pistons to allow for CO pumping in channel A while channel B adds organic modifier for either isocratic or gradient performance.
  • Page 15 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II SFC Multisampler (G4767A) In SFC, the complete solvent flow path needs to be pressurized under all conditions to avoid expansion of the supercritical fluid. With the Feed Injection Technology the sample volume is drawn under atmospheric pressure conditions, pressurized to system pressure, and injected by an ultrafast syringing process.
  • Page 16 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II Multicolumn Thermostat (G7116A) Product Description The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) allows precise column thermostatting over a broad temperature range: from cooling down to 10 below ambient temperature to heating up to 85 °C, thus providing high flexibility for optimized speed and selectivity in standard LC separation.
  • Page 17 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System significantly affect noise levels. Temperature changes of mobile phase with the left side heat exchanger e.g. between 38 °C and 49 °C resulted in a variation in peak-to-peak noise of over an order of magnitude.
  • Page 18 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System The Agilent 1260 Infinity II Diode Array Detector WR (G7115A) and Agilent 1260 Infinity II Multi Wavelength Detector (G7165A) The system can be equipped either with a DAD or an MWD using a high pressure detector flow cell suitable for SFC (10 mm path length, 13 μL volume), with short transfer tubing to minimize peak broadening.
  • Page 19 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System Columns In contrast to reversed phase separation, there is no universal stationary phase available for SFC separations. Most typically used stationary phases are ethyl pyridine, diol, cyano, amino, Silica and SCX columns. This usually leads to additional effort to screen different columns in order to achieve optimum separation.
  • Page 20 Introduction to Supercritical Fluid Chromatography (SFC) The Agilent 1260 Infinity II SFC System...

  • Page 21: Site Requirements And Specifications

    Site Requirements Power Considerations Power Cords Bench space Environment Ventilation Specifications System Specifications Specifications Agilent 1260 Infinity II System This chapter provides information on environmental requirements, physical and performance specifications only for the Agilent 1260 Infinity II SFC System. Agilent Technologies...

  • Page 22: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in “System Specifications”...

  • Page 23: Power Cords

    Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 24 Site Requirements and Specifications Site Requirements The module is designed to be installed on either side of the 1260 Infinity II system stack with sufficient high pressure transfer tubing to attach to a double-stacked system. If the 1260 Infinity II system is attached to a split-flow detector such as Mass Spec or ELSD, the Agilent 1260 Infinity II SFC Control Module should be positioned on the opposite side of the stack.

  • Page 25: Environment

    Site Requirements and Specifications Site Requirements Environment Condensation within the module C AU T I O N Condensation can damage the system electronics. ➔ Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module. ➔...

  • Page 26: Specifications

    Site Requirements and Specifications Specifications Specifications System Specifications Table 1 1260 Infinity II SFC System Specifications Type Specifications Comments Settable flow range 0.001 mL/min to 5 mL/min recommended: 1 mL/min to 5 mL/min Maximum operating pressure 600 bar up to 5 mL/min Upgrade of existing Infinity and Infinity II setups Option to run SFC/UHPLC in...
  • Page 27 Site Requirements and Specifications Specifications Table 2 Physical Specifications (G4301A) Type Specification Comments Weight 26 kg (56 lbs) Dimensions 60 cm x 26 cm x 48 cm adapted to new data sheet (height × width × depth) (23 in x 10 in x 18 in) units Line voltage 100 - 240 VAC, ±10 %...
  • Page 28 Site Requirements and Specifications Specifications Table 3 Chemical Specifications (G4301A) Type Specifications Inlet CO bulk purity >99.99 % vapor; >99.999 % liquid Inlet CO phase vapor from non-dip-tube high pressure cylinder; liquid from commercial CO delivery system Inlet CO supply pressure 40 - 70 bar (580 - 1000 psi) Inlet CO...
  • Page 29 Site Requirements and Specifications Specifications Performance Specifications Table 5 Performance Specifications (G4301A) Type Specifications Hydraulic system Single piston with proprietary motor control Total hydraulic volume <5 mL @ pressure <70 bar <25 mL @ pressure up to 400 bar Chiller system Thermoelectric cooling with secondary air/liquid cooling circuit Chiller temperature...

  • Page 30: Agilent 1260 Infinity Ii System

    Site Requirements and Specifications Specifications Agilent 1260 Infinity II System Unspecified Conditions WA R N I N G Operating the instrumentation under conditions other than its intended use might result in a potential safety hazard or might damage the instrumentation. ➔...
  • Page 31 Installing the Agilent 1260 Infinity II SFC System Hardware Installation General Procedures Installing the Agilent 1260 Infinity II SFC Control Module (G4301A) Preparing the HPLC This chapter provides an overview of the installation and setup of the hardware and software Agilent Technologies...

  • Page 32: Installing The Agilent 1260 Infinity Ii Sfc System Hardware Installation

    Installing the Agilent 1260 Infinity II SFC System Hardware Installation Hardware Installation General Procedures Proper use of wrenches Some of the plumbing connections require a nut to be tightened onto a fitting. There are often two sets of flats next to each other. Attempting to tighten the nut without securing the other part of the fitting with a second wrench can result in loosening yet another connection upstream or downstream.
  • Page 33 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Compression (Swaged Fittings) The fittings used in the Agilent 1260 Infinity II SFC Control Module are Valco. Fittings used in all Agilent modules are Swagelok. Use the appropriate fitting as recommended by the equipment manufacturer. The recommended tightening procedure to install new fittings is to tighten the nut finger tight, then an additional 1/4 to 1/2 turn to seal.
  • Page 34 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Compression fittings used in the SFC control module use a nut and ferrule. As the nut is tightened the ferrule compresses on the tubing creating a leak-tight seal When correctly installed, there is minimal additional volume.
  • Page 35 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Installing the Agilent 1260 Infinity II SFC Control Module (G4301A) Early connection may damage the instrument C AU T I O N ➔ Do not connect AC power or interconnection cables or gas tubing to the Agilent 1260 Infinity II SFC Control Module until these installation procedures direct you to do so.
  • Page 36 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Delivery Checklist Compare the delivery checklist with the contents of the shipping boxes to ensure completeness of the shipment. Connecting the Booster to the Agilent 1260 Infinity II system Locate the stainless steel booster transfer line. Connect one end to the top port of the output tee on the center right side of the booster drawer.
  • Page 37 Installing the Agilent 1260 Infinity II SFC System Hardware Installation This tube can be routed through the tee slots on the side of the Agilent 1260 Infinity II SFC Control Module. The tube is routed upward between the SFC control module and the Agilent 1260 Infinity II stack to the bottom side of the binary pump.
  • Page 38 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Install the Multisampler Connect capillary from SFC BinPump outlet to port 1 in SFC Multisampler's injection valve and capillary to MCT to port 6. Figure 12 Plumbing of the SFC Multisampler Optimized Thermostatted Column Compartment Plumbing Supercritical Fluid Chromatography (SFC) is susceptible to increased noise due to poor thermal matching of components within the Agilent 1260 Infinity...
  • Page 39 Thus, the detector flow cells (or splitter in the case on an ELSD or Mass Spectrometer), operate at an elevated pressure relative to HPLC. Agilent Technologies offers a Diode Array Detector (DAD) flow cell that has been extensively optimized for use in Supercritical Fluid Chromatography (SFC).
  • Page 40 Installing the Agilent 1260 Infinity II SFC System Hardware Installation The flow cell should already be installed in the DAD/MWD. Carefully examine the inlet and outlet ports of the cells to ensure that flow is in the correct direction. In the DAD/MWD, the outlet port is normally located below the inlet port on the connection block.
  • Page 41 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Figure 15 Plumbing of the flow cell...
  • Page 42 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Connecting the BPR to the 1260 Infinity II stack Connect the Agilent 1260 Infinity II SFC Control Module return transfer tube to the outlet block of the detector. This tube can then be routed out the concave opening in the bottom of the detector behind the detector cover.
  • Page 43 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Connecting the Waste system to the SFC control module BPR Outlet The Agilent 1260 Infinity II SFC Control Module has a waste bottle located outside of the cabinet. It can be located anywhere easily accessible and visible within the range of the supplied tubing.
  • Page 44 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Exposure to toxic substances WA R N I N G ➔ The vapor exiting the module may contain several percent organic solvent. The effluent should NEVER be vented directly into an enclosed space occupied by humans because of the potential for long-term exposure to toxic substances.
  • Page 45 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Connecting the Leak Tray Waste Line The Agilent 1260 Infinity II SFC Control Module contains a leak tray on the bottom of the instrument to collect and sense any liquid spills that may occur in the Agilent 1260 Infinity II SFC Control Module cabinet.
  • Page 46 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Cable connections to the HPLC Personal injury WA R N I N G ➔ Ensure that the AC power cord is NOT yet connected to the instrument. Remote signal USB to PC Figure 19 Remote Connection 5188-8045 Remote Cable APG-ERI Power off...
  • Page 47 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Lastly, a cable should be connected to the Agilent 1260 Infinity II SFC Control Module USB port. This can be run to any convenient, compatible USB port on the PC. CAN cabling between the Agilent 1260 Infinity II modules is unchanged from the normal, recommended means of interconnecting HPLC devices.
  • Page 48 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Connecting the Agilent 1260 Infinity II SFC Control Module to a Source of Carbon Dioxide The Agilent 1260 Infinity II SFC Control Module has a 1/8 inch tube inlet connection on the lower left side on the back of the module. This connection is actually part of a very high surface area filter intended to intercept catastrophic levels of particulates.
  • Page 49 They do not appear to change at national borders (some users in one country have different supply connections from other users in the same country). Agilent Technologies recommends that customers in Europe contact their gas supply companies and ask them how to mate the...
  • Page 50 Installing the Agilent 1260 Infinity II SFC System Hardware Installation require a cylinder connector and a reducing union down to a 1/8 inch compression fitting. Leaks will not be sensed or protected WA R N I N G ➔ Carefully check supply cylinders and inlet fittings for leaks. Any leaks present in the supply line and inlet fitting will not be sensed or protected by safety features in the module.
  • Page 51 1500 psi (>100 bar). While this is rating is well above the outlet pressure of any typical GDS, past experience suggests that it is wise to allow for some extra margin. Agilent Technologies suggests setting the local output of any GDS between 60 bar and 70 bar, through local outlet pressure regulators.
  • Page 52 Installing the Agilent 1260 Infinity II SFC System Hardware Installation System Flow Diagram Figure 21 on page 52 schematically shows the correct plumbing of the SFC system. Figure 21 SFC System flow diagram...
  • Page 53 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Preparing the HPLC Preferred Stack Arrangement To minimize delays and broadening caused by excessive tube lengths, we recommend the following stack layout (the Agilent 1260 Infinity II SFC Control Module needs to be immediately adjacent to the LC stack, but can be placed on either side): SFC Control module Solvent cabinet...
  • Page 54 Installing the Agilent 1260 Infinity II SFC System Hardware Installation Agilent 1260 Infinity II SFC Binary Pump It is highly recommended that you familiarize yourself with standard maintenance functions and terminology used in the binary pump. This information is available in the Agilent Binary pump manual. Less Polar Sample Solvents Help Replacing the sample solvent with a much less polar solvent can allow injection of much larger volumes.

  • Page 55: Configuring The System

    Configuring the System Configuring the SFC system in OpenLAB CDS Setting up the Method Status Control How to configure the Agilent 1260 Infinity II SFC System and Agilent 1260 Infinity II SFC Control Module in OpenLAB CDS. Agilent Technologies...

  • Page 56: Configuring The Sfc System In Openlab Cds

    Configuring the System Configuring the SFC system in OpenLAB CDS Configuring the SFC system in OpenLAB CDS The Agilent 1260 Infinity II SFC system requires the ChemStation Modular LC System, not N O T E with Modular LC System (Classic). The Modular LC System (Classic) does not support the RC.Net drivers, which are necessary for the SFC modules.
  • Page 57 Configuring the System Configuring the SFC system in OpenLAB CDS 3 In the upper panel of the Configure Instrument dialog box, ensure that the Classic drivers check box is cleared. If your instrument is not supported by the classic ChemStation Edition drivers, the Use N O T E classic drivers check box is unavailable.
  • Page 58 Configuring the System Configuring the SFC system in OpenLAB CDS 5 To set up the SFC module, scroll down the Configurable Modules panel until you see the SFC icon. Move it to the Selected Modules panel either by double-clicking it or selecting it and clicking the right-arrow button. 6 Select the SFC module in the Selected Modules panel and click Configure.
  • Page 59 Configuring the System Configuring the SFC system in OpenLAB CDS The SFC Configuration dialog box is displayed. 7 Click the COM Port down-arrow and select the COM port through which the SFC Control Module communicates with the ChemStation. The Device name is the name that is shown in all ChemStation reports. You can edit the default name if you wish.

  • Page 60: Setting Up The Method

    Configuring the System Setting up the Method Setting up the Method 1 In the Agilent ChemStation, the method parameters for all RC.Net devices, including SFC settings, are combined into a single tabbed dialog box:...
  • Page 61 Configuring the System Setting up the Method 2 The Instrument Curves tab shows all instrument curves (monitor traces) available for storing with the acquired data file:...

  • Page 62: Status

    Configuring the System Status Status 1 The SFC System status is displayed in the Dashboard. You can toggle the display of the Dashboard using View > System diagram. 2 Click in the module's title bar to display the instrument actuals. Click to hide the instrument actuals display.

  • Page 63: Control

    Configuring the System Control Control The following steps show you how you can control your SFC instrument in the Agilent OpenLAB CDS ChemStation Edition. 1 In Instrument > More SFC, and in the context menu (right-click) of the SFC Dashboard panel are more menu items that allow you to control the device directly.
  • Page 64 Configuring the System Control...

  • Page 65: Using The Agilent 1260 Infinity Ii Sfc Control Module

    Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent OpenLAB CDS Running a method on the SFC system Shutting Down the SFC System Partial Shutdown This chapter provides information and hints on the use of the SFC System. Agilent Technologies...

  • Page 66: Powering Up The Module

    Using the Agilent 1260 Infinity II SFC Control Module Powering up the Module Powering up the Module The Agilent 1260 Infinity II SFC Control Module is powered on by pressing the top of the rocker-type power switch located on the upper right rear of the module.

  • Page 67: Power-Up Sequence And Operational Control States

    Using the Agilent 1260 Infinity II SFC Control Module Power-up Sequence and Operational Control States Power-up Sequence and Operational Control States When power is applied to the Agilent 1260 Infinity II SFC Control Module, a series of events is initiated. The order of these events is designed to safely initialize and test individual component functionality.

  • Page 68: Operational Control States

    Using the Agilent 1260 Infinity II SFC Control Module Operational Control States Operational Control States The Agilent 1260 Infinity II SFC Control Module has three defined operational control states: OFF, STANDBY and ON. You control the three states by selecting the Control option from either the Open Lab CDS or from the SFC Test Utility Software.

  • Page 69: The Standby State

    Using the Agilent 1260 Infinity II SFC Control Module Operational Control States The OFF state is always entered after a successful power-up sequence. It can also be entered by selecting the Off option in the control window, by a timeout from the STANDBY state or by pressing Off in the Agilent ChemStation graphical user interface twice in succession.

  • Page 70: The On State

    Using the Agilent 1260 Infinity II SFC Control Module Operational Control States The ON State The ON state is characterized as follows: ✔ The CO supply valve is open (powered). ✔ The booster pump drive is powered and begins to pump CO to the pressure target.

  • Page 71: Controlling The Agilent 1260 Infinity Ii Sfc Control Module Through The Agilent Openlab Cds

    Using the Agilent 1260 Infinity II SFC Control Module Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent OpenLAB CDS Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent OpenLAB CDS Figure 23 Editing the SFC control module Method Parameters Editable method parameters for the Agilent 1260 Infinity II SFC Control Module are the BPR Pressure (system back-pressure) and the BPR Temperature (temperature of the back-pressure regulator).
  • Page 72 Using the Agilent 1260 Infinity II SFC Control Module Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent OpenLAB CDS When the SFC Mode check box is unchecked the SFC Control Module goes to Standby but reports Ready state to the System. In this mode HPLC runs can be made without any CO flow.

  • Page 73: Running A Method On The Sfc System

    Using the Agilent 1260 Infinity II SFC Control Module Running a method on the SFC system Running a method on the SFC system The OpenLAB CDS ChemStation installation includes a default method, DEF_LC.M , which you should use as the basis of your SFC method. DEF_LC.M cannot be overwritten;...
  • Page 74 Using the Agilent 1260 Infinity II SFC Control Module Running a method on the SFC system Default Pump setting is 80 % CO : 20 % modifier. When you select CO as the solvent, the Compressibility is set automatically. Typical Flow rate in SFC is 3 mL/min.

  • Page 75: Shutting Down The Sfc System

    Using the Agilent 1260 Infinity II SFC Control Module Shutting Down the SFC System Shutting Down the SFC System The manner of shutting down the SFC depends on the requirement for rapid equilibration of the system on the next startup, and the duration of the shutdown.
  • Page 76 Using the Agilent 1260 Infinity II SFC Control Module Shutting Down the SFC System Depressurizing the system When maintenance is required on the SFC system, such as replacing the column, the system should be depressurized. It is not necessary to shut down all modules, but only the pumps and any other devices undergoing maintenance.

  • Page 77: Maintenance And Repair

    Standard Decontamination Plugged BPR Decontamination Preparing for storage or shipping In this chapter only the SFC specific procedures are described. For procedures similar to the Agilent module procedures, please refer to the single module manuals (G4782A, G4767A, G7116A, G7115/65A) Agilent Technologies...

  • Page 78: Inspection And Preventative Maintenance Intervals

    Maintenance and Repair Inspection and Preventative Maintenance Intervals Inspection and Preventative Maintenance Intervals Inspection and maintenance of the Agilent 1260 Infinity II Analytical SFC System are critical elements of long term reliability and performance of the system. Maintenance falls into two categories, preventative and corrective. Preventative maintenance intervals can vary based on the system use.

  • Page 79: Every 3 Months

    Maintenance and Repair Inspection and Preventative Maintenance Intervals • If an inlet step- down regulator is used with a gas supply system, check that the inlet pressure is between 40 and 70 bar. • Check the integrity of the SFC Flow path (that is, that column, flow cell etc have not been removed).

  • Page 80: General Maintenance Procedures

    Maintenance and Repair General Maintenance procedures General Maintenance procedures Booster Drawer Piston Piston seal Outlet valve type N Booster pump head SFC Inlet valve Thermal insulator Figure 26 Exploded view of Booster Components...
  • Page 81 Maintenance and Repair General Maintenance procedures Removing the vapor shield The vapor shield is not shown in the graphic Figure 26 on page 80. Most maintenance procedures require removal of the vapor shield to access the underlying pump unit. Tools required •...
  • Page 82 Maintenance and Repair General Maintenance procedures Replacing the vapor shield 1 Locate the vapor shield approximately over the mounting holes in the booster drawer front panel. 2 Engage each screw approximately one turn. 3 Inspect the border of the vapor shield to make sure it is in sealing contact with the foam seal of the drawer face.
  • Page 83 Maintenance and Repair General Maintenance procedures Danger of piston breakage C AU T I O N ➔ Be careful not to break the piston when removing the pump head. Twisting the pump head can cause the piston to break. 6 Using the 3/16” hex-drive, carefully remove the two knurled nuts at the front of the pump head.
  • Page 84 Maintenance and Repair General Maintenance procedures Replacing the Piston Seal 1 Sonicate or soak the new seal in isopropanol for 15 min to clean and provide lubrication for installing. 2 Place the replacement seal on the rod-shaped end of the seal insertion/removal tool so that the spring is visible when the seal is fully seated on the tool.
  • Page 85 Maintenance and Repair General Maintenance procedures Cleaning or Replacing Booster Pump Piston Tools required • Tools for removing the vapor shield (see “Removing the vapor shield” on page 81) and pump head (see “Removing the Pump head” on page 82) •...
  • Page 86 Maintenance and Repair General Maintenance procedures 8 Grasp the metal base of the replacement piston assembly, and insert it into the slot in the piston carrier until it bottoms in the slot. 9 Replace the retaining ring and spacer. Reattach the spacer mounting screws.

  • Page 87: Replacing Fuses

    Maintenance and Repair General Maintenance procedures Replacing Fuses The power entry module of the Agilent 1260 Infinity II SFC Control Module unit contains an external fuse drawer that is user serviceable. To replace fuses: (before replacing fuses, first try to determine cause of fuse activation and repair) Parts required Description...

  • Page 88: Standard Decontamination

    Maintenance and Repair General Maintenance procedures Standard Decontamination Cleaning External surfaces of the enclosure can be wiped with a damp soft cloth. More stubborn marks can be removed with a 50 % isopropanol:water mixture or mild cleanser such as Soft Scrub™. The latter may also be used to remove surface paint blemishes that may result from normal use.

  • Page 89: Plugged Bpr Decontamination

    Maintenance and Repair General Maintenance procedures Plugged BPR Decontamination Decontamination of plugged BPR heads may require more aggressive solvents. In this case use the following procedure: 1 Depressurize the SFC Control Module completely. 2 Disconnect the BPR inlet and outlet tubes from the BPR drawer. 3 Attach the Inlet tube via a transfer line to waste.
  • Page 90 Maintenance and Repair General Maintenance procedures 7 Coil the lines to fit in the SFC control module behind the removable front panel. 8 Disconnect the booster pump transfer line from the binary pump at the pump inlet check valve. 9 Disconnect the BPR return line from the detector. 10 Coil both lines to fit inside the SFC control module behind the removable front panel.

  • Page 91: Parts For Maintenance

    Parts for Maintenance Agilent 1260 Infinity II SFC Control Module Parts Agilent 1260 Infinity II SFC DAD Parts This chapter provides information on parts for maintenance and repair. Agilent Technologies...

  • Page 92: Agilent 1260 Infinity Ii Sfc Control Module Parts

    Parts for Maintenance Agilent 1260 Infinity II SFC Control Module Parts Agilent 1260 Infinity II SFC Control Module Parts G4301A SFC Control Module Description G4301-65000 Mainboard G4301A G4301-65010 Motor Drive Board G4301-60501 Heated Nozzle Assembly LD G4301-00200 Thermal Isolation Cover Assy G4301-67009 BPR Drawer G4301-67028...
  • Page 93 Parts for Maintenance Agilent 1260 Infinity II SFC Control Module Parts G4301-67028 Booster Drawer Description parts G4301-60056 Capillary SFC Booster Pump out G4302-60066 Inlet valve SFC Pump G4301-60101 Thermal Insulator Assy G4301-20200 Seal SFC Booster G4301-20201 Piston SFC Booster G4301-20100 Pump Head SFC Booster G1312-60167 Outlet valve 1260 N-Type...

  • Page 94: Agilent 1260 Infinity Ii Sfc Dad Parts

    Parts for Maintenance Agilent 1260 Infinity II SFC DAD Parts Agilent 1260 Infinity II SFC DAD Parts Figure 27 DAD Flow Cell SFC Item Description G4301-60100 SFC Flow Cell G4301-60200 SFC Low Dispersion Flow Cell 79883-68700 High Pressure Flow cell refurbishment Kit For all other Part Numbers please refer to the G7115/65A User manual.

  • Page 95: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the modules. Agilent Technologies...

  • Page 96: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
  • Page 97 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) RS-232 cables (not for FUSION Description board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).

  • Page 98: Analog Cables

    Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
  • Page 99 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...

  • Page 100: Remote Cables

    Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) 5188-8029 ERI to general purpose p/n 5188-8029 Color code Enhanced Classic Active (TTL) Remote Remote white START REQUEST brown STOP green READY High yellow POWER ON High grey NOT USED pink SHUT DOWN blue...
  • Page 101 Identifying Cables Remote Cables 5188-8044 ERI to ERI (Connector D_Subminiature 15 pin) Table 6 5188-8044 ERI to ERI p/n 5188-8044 Pin (ERI) Signal Pin (ERI) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector...
  • Page 102 High Start High Prepare High Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.
  • Page 103 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...

  • Page 104: Can/Lan Cables

    Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...

  • Page 105: Cables

    Identifying Cables RS-232 Cables RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

  • Page 106: Usb

    Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive)

  • Page 107: Appendix

    Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) Radio Interference Sound Emission Solvent Information Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...

  • Page 108: General Safety Information

    Appendix General Safety Information General Safety Information Safety Symbols Table 8 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.
  • Page 109 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
  • Page 110 Appendix General Safety Information Operation Before applying power, comply with the installation section. Additionally the following must be observed. Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto-transformers, and devices connected to it must be connected to a protective earth via a ground socket.
  • Page 111 Appendix General Safety Information Capacitors inside the instrument may still be charged, even though the instrument has been disconnected from its source of supply. Dangerous voltages, capable of causing serious personal injury, are present in this instrument. Use extreme caution when handling, testing and adjusting. When working with solvents, observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when...

  • Page 112: The Waste Electrical And Electronic Equipment (Weee) Directive (2002-96-Ec)

    Appendix The Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) The Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005.

  • Page 113: Radio Interference

    Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...

  • Page 114: Sound Emission

    Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 115: Solvent Information

    Appendix Solvent Information Solvent Information Flow Cell To protect optimal functionality of your flow-cell: • Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of the flow cell.
  • Page 116 Appendix Solvent Information Use of Solvents Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Avoid the use of the following steel-corrosive solvents: • Solutions of alkali halides and their respective acids (for example, lithium iodide, potassium chloride, and so on), •...

  • Page 117: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com...
  • Page 118 Index Index Agilent overview on internet cable algae power consideration cable power cords overview remote RS-232 cables cables RS-232C analog cable overview remote safety class I cable safety condensation general information symbols site requirements flow cell solvents solvent information Specifications installation power cords site requirements...
  • Page 120 • Configuring the System • Using the Agilent 1260 Infinity SFC Control Module • Maintenance • Parts for Maintenance • Identifying Cables • Appendix © Agilent Technologies 2010 - 2016, 2017 Printed in Germany 02/2017 *G4301-90104* *G4301-90104* G4301-90104 Agilent Technologies...

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