Concept Of Time Triggering - Agilent Technologies 1290 Infinity II 2D-LC Solution User Manual

2 Concepts of 2D-LC
Triggering of 2D-LC

Concept of Time Triggering

Time-triggered LC-LC
One or more parts of the 1
onto the 2D-column.
Figure 12
Time triggered LCxLC
In time-triggered LCxLC the user can set start and end-times of second
dimension sampling in the software, multiple time segments can be used. This
can be used for example if the samples are known with known areas without
peaks being eluted from the first dimension. Or, to save solvent and reduce valve
wear for the time before the first peak is eluted from the first dimension column
or during its wash-out or re-equilibration time. The software allows to set an idle
flow rate for these times.
Figure 13
2D-LC - User Guide
st
dimension in given time frames are directly injected
Principles of time-triggered LC-LC
1
D chromatogram
Principle of time-triggered LCxLC
1
D chromatogram
2
D sampling
2
D gradient
2
D sampling
2
D gradient
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