Positive Population Selected From The Single-Stained Sample - Beckman Coulter CytoFLEX SRT Instructions For Use Manual

Compensation
Creating a Compensation Experiment
CAUTION
Risk of erroneous results. Calculations based on excessively small volumes of
sampled data can be inaccurate. Ensure that more than 1,000 positive events and
more than 1,000 negative events are sampled. If the number of positive cells is
comparatively low, increase the number of acquisition events to a suitable
amount.
4
Move the gate in the FSC/SSC plot so that it encloses the desired population. Move the positive
gate in the plot so that it encloses the positive population.
NOTE
Figure 6.3
is defined by the unstained sample.
Figure 6.3 Positive Population Selected from the Single-Stained Sample
1. Positive population
6-8
shows an example of selecting the positive population when the negative population
1
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