Beckman Coulter CytoFLEX SRT Instructions For Use Manual page 382

Cell sorter
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Table 9.1 Troubleshooting-[Error Codes] (Continued)
Problem
[Error code 060049] The charge
phase value is out of range.
C37808AC
Probable Cause
• The fluid stream is unstable.
• The photodiodes are defective.
• The charge drive board is defective.
Corrective Action
1. Select Standby to turn off the sheath.
2. Verify that the nozzle is clean and O-ring is present.
• Clean the nozzle if needed. Refer to
Nozzle
in
CHAPTER 10, Cleaning
• If necessary, replace the O-ring. Refer to
Replacing the O-Ring
in
Replacement/Adjustment
• If the nozzle is damaged, replace the nozzle.
Refer to
Replacing the Nozzle
Replacement/Adjustment
3. Reinstall the nozzle module. Refer to
Installing the Nozzle Module
Replacement/Adjustment
Procedures.
4. Run Flow Cell Debubble. Refer to
Flow Cell De-bubble
in
CHAPTER 11, Replacement/
Adjustment
Procedures.
5. Run Sheath Filter debubble. Refer to
Sheath Filter De-bubble
in
Replacement/Adjustment
Procedures.
6. Run Daily Clean. Refer to
Daily Clean Program
CHAPTER 10, Cleaning
Procedures.
7. Run Flow cell Clean. Refer to
Program
in
CHAPTER 10, Cleaning
8. Clean the side stream illumination and the deflection
plates. Refer to
Cleaning the Side Stream
Illumination Source and the Deflection Plates
CHAPTER 10, Cleaning
Procedures.
9. Wipe the side stream detection window. Refer to
Figure
1.23.
10. Verify that the nozzle lift is clean and dry. Refer to
Steps
4
-
6
in
CHAPTER 10, Cleaning
11. If the problem persists,
contact
9
Troubleshooting
Troubleshooting Table
Cleaning the
Procedures.
CHAPTER 11,
Procedures.
in
CHAPTER 11,
Procedures.
Removing/
in
CHAPTER 11,
Performing the
Performing the
CHAPTER 11,
in
Flow Cell Clean
Procedures.
in
Procedures.
us.
9-11

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