System Overview; Overview; Product Description; Operation Principles - Beckman Coulter CytoFLEX SRT Instructions For Use Manual

Overview

This chapter describes the individual components of the CytoFLEX SRT instrument and the
corresponding functions of these components.
This chapter contains information on:

Product Description

•
• Main Components
• Fluidics System
• Optical Components
• Cell Sorting
System Connections
•
• Instrument Specifications
• Performance Characteristics
Material Safety Data Sheets (SDS/MSDS)
•
Product Description
For Research Use Only. Not for use in diagnostics procedures. The use of data generated by this
instrument depends upon the regulatory status of the reagents used.
The CytoFLEX SRT Cell Sorter is a research instrument that analyzes and sorts cellular suspensions
and other similarly sized particle suspensions.
The system can be ordered in various configurations from 2 lasers, 5 colors, to a maximum of 4
lasers, 15 colors.

Operation Principles

The CytoFLEX SRT Sorter examines individual particles that are propelled in saline sheath through
a flow cell (1), then through one to four spatially separated laser beams (2) of differing wavelengths.
If the properties of the particle, or fluorescent dye added to the particle, are excited by the
wavelength of laser light, the particle emits broadband fluorescence and scattered light. The
emitted light is collected, focused, reflected, and filtered, so that discrete wavelengths of light are
detectable by the fiber array photo detectors (FAPD). The FAPD converts the light signals to
electronic signals that are sent to the instrument electronics. Data is then acquired by CytExpert
SRT software according to the parameters set by the operator.
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CHAPTER 1

System Overview

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