Beckman Coulter CytoFLEX SRT Instructions For Use Manual page 392

Cell sorter
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Table 9.1 Troubleshooting-[Error Codes] (Continued)
Problem
[Error code 060093] The central
stream is not aligned with the
detectors.
C37808AC
Probable Cause
• The fluid stream is unstable.
• The photodiodes are defective.
• The nozzle is clogged or damaged.
• Air bubbles are in the system.
• Airflow interference.
• Leakage of the fluidics system.
• Electric leakage of high-voltage
components.
• The narrow slot mask of the PD is
partly or completely blocked.
• The side stream detector or gap
detector are not aligned with the core
stream.
Corrective Action
1. Select Standby to turn off the sheath.
2. Wipe the side stream detection window. Refer to
Figure
1.23.
3. Verify that the nozzle is clean and O-ring is present.
• Clean the nozzle if needed. Refer to
Nozzle
in
CHAPTER 10, Cleaning
• If necessary, replace the O-ring. Refer to
Replacing the O-Ring
in
Replacement/Adjustment
• If the nozzle is damaged, replace the nozzle.
Refer to
Replacing the Nozzle
Replacement/Adjustment
4. Reinstall the nozzle module. Refer to
Installing the Nozzle Module
Replacement/Adjustment
Procedures.
5. Run Flow Cell Debubble. Refer to
Flow Cell De-bubble
in
CHAPTER 11, Replacement/
Adjustment
Procedures.
6. Run Sheath Filter debubble. Refer to
Sheath Filter De-bubble
in
Replacement/Adjustment
Procedures.
7. Run Daily Clean. Refer to
Daily Clean Program
CHAPTER 10, Cleaning
Procedures.
8. Run Flow cell Clean. Refer to
Program
in
CHAPTER 10, Cleaning
9. Verify that the Sorter is not exposed directly to a
ventilation outlet of an air condition.
10. If the problem persists,
contact
9
Troubleshooting
Troubleshooting Table
Cleaning the
Procedures.
CHAPTER 11,
Procedures.
in
CHAPTER 11,
Procedures.
Removing/
in
CHAPTER 11,
Performing the
Performing the
CHAPTER 11,
in
Flow Cell Clean
Procedures.
us.
9-21

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