Beckman Coulter CytoFLEX SRT Instructions For Use Manual page 387

Troubleshooting
Troubleshooting Table
Table 9.1 Troubleshooting-[Error Codes] (Continued)
Problem
[Error code 060058] Failed to
optimize the charge voltage for
L2 stream.
[Error code 060059] Failed to
optimize the charge voltage for
L1 stream.
[Error code 060060] Failed to
optimize the charge voltage for
R1 stream.
[Error code 060061] Failed to
optimize the charge voltage for
R2 stream.
[Error code 060062] Failed to
optimize the charge voltage for
central stream.
9-16
Probable Cause
• The fluid stream is unstable.
• The photodiodes are defective.
• Airflow interference.
• Electric leakage of high-voltage
component.
• Leakage of the fluidics system.
• The narrow slot mask of the PD is
partly or completely blocked.
• The core stream or the side stream
Hardware is not straight enough.
Corrective Action
1. Select Standby to turn off the sheath.
2. Verify that the nozzle is clean and O-ring is present.
• Clean the nozzle if needed. Refer to
Nozzle in CHAPTER 10, Cleaning
• If necessary, replace the O-ring. Refer to
Replacing the O-Ring in
Replacement/Adjustment
• If the nozzle is damaged, replace the nozzle.
Refer to Replacing the Nozzle
Replacement/Adjustment
3. Reinstall the nozzle module. Refer to
Installing the Nozzle Module
Replacement/Adjustment Procedures.
4. Run Flow Cell Debubble. Refer to
Flow Cell De-bubble in CHAPTER 11, Replacement/
Adjustment Procedures.
5. Run Sheath Filter debubble. Refer to
Sheath Filter De-bubble in
Replacement/Adjustment Procedures.
6. Run Daily Clean. Refer to Daily Clean Program
CHAPTER 10, Cleaning Procedures.
7. Run Flow cell Clean. Refer to
Program in CHAPTER 10, Cleaning
8. Clean the side stream illumination and the deflection
plates. Refer to Cleaning the Side Stream
Illumination Source and the Deflection Plates
CHAPTER 10, Cleaning Procedures.
9. Wipe the side stream detection window. Refer to
Figure 1.23.
10. Verify that the nozzle lift is clean and dry. Refer to
Steps 4 - 6 in CHAPTER 10, Cleaning
11. Ensure that the ambient temperature in the lab is
stable and within the range (15-27 ℃), and the
fluctuation is within < ±2 °C. Use appropriate air
condition if necessary.
12. Verify that the Sorter is not exposed directly to a
ventilation outlet of an air condition.
13. Rerun Sort Calibration. Refer to
in CHAPTER 5,
(Auto Drop Delay)
14. If the problem persists, contact
Cleaning the
Procedures.
CHAPTER 11,
Procedures.
in CHAPTER 11,
Procedures.
Removing/
in CHAPTER 11,
Performing the
Performing the
CHAPTER 11,
in
Flow Cell Clean
Procedures.
in
Procedures.
Sort Calibration
Sorting.
us.
C37808AC