Beckman Coulter CytoFLEX SRT Instructions For Use Manual page 169

Instrument Quality Control and Standardization
Quality Control
During QC, the software automatically seeks the CytoFLEX QC Fluorospheres and computes the
results. The software returns to the QC Report screen after the QC run is complete.
8
If the sampling rate is too low, the Sorter stops the QC run and displays a prompt alerting you
that the QC run failed to reach the required event flow rate. This is not considered a QC failure.
If this situation occurs, increase the sample concentration by adding one drop of CytoFLEX
Daily QC Fluorospheres to the sample tube or prepare a new tube of CytoFLEX Ready to Use
Daily QC Fluorospheres, and then perform the QC.
NOTE
Mix the fluorospheres dropper bottle thoroughly before dispensing it into a sample tube.
9
If the lot number of CytoFLEX QC Fluorospheres is new and QC fails, the following software
message appears. Select
NOTE
Target gain values must be established for each new lot number of CytoFLEX QC Fluorospheres.
QC could fail up to 3 times upon running each new lot number for the first time until target gain
values are established.
If the lot number of CytoFLEX QC Fluorospheres is NOT new and QC fails, refer to Step
Confirming
If QC passes, proceed to Step 10.
4-12
.
Yes
Results, or CHAPTER 9, Troubleshooting.
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