Calculating Sample Concentration - Beckman Coulter CytoFLEX SRT Instructions For Use Manual

Data Review

Calculating Sample Concentration

Calculating Sample Concentration
The CytoFLEX SRT instrument supports the calculation of the sample concentration based on the
known concentration of the reference beads. The system does not support the direct calculation of
concentration based on the volume.
NOTE
If the Events/μL(V) checkbox is selected, the Events/μL(V) column displays #### in the statistics
table. However, the system is compatible with the experiments using other CytExpert software. Select
the Events/μL(V) checkbox to display the Events/μL(V) value if needed.
• Select
Statistics
• Right-click on the statistics table and select
appears.
• Select the Statistics tab. Refer to
• Select the Events/μL(B) checkbox and select the gated
• Enter the total number of reference beads as the
total.
NOTE
You can also enter the reference bead concentration directly in the Beads Count field and set the
Sample Volume as 1.
• The software automatically calculates the original sample concentration based on the input
values.
To obtain accurate calculations, throughout the data acquisition process, ensure that:
• The sample concentration is 2 x 10
• Samples are thoroughly mixed before loading and that they exhibit no apparent subsidence
throughout the testing process.
• The detection rate is maintained at less than 10,000 events/second throughout the sampling
process.
• A constant sampling rate is maintained when recording data.
• You acquire at least 10 μL of sampling volume.
NOTE
While collecting samples, instantaneous data calculation can appear inaccurate. Regard the
calculation as accurate only after data acquisition has been completed.
7-6
in the graphic controls tool bar to create a statistics table.
Figure 7.1.
4 7
-10 units/mL.
. The Statistics Setting window
Statistics Setting
Beads Population
, as well as the
Beads Count
.
in
Sample Volume
C37808AC