Drop Envelope; Sort Precision Modes Applied To The Same Sort Stream - Beckman Coulter CytoFLEX SRT Instructions For Use Manual

(red highlight) depending on the sort precision level chosen. The sort precision levels are showed
from left to right:
Figure 2.13 Sort Precision Modes Applied to the Same Sort Stream

Drop Envelope

The sort drop envelope defines how many drops are sorted based on the position of the positive
event in the drop. Refer to
want a more stringent event location (1/2 Drop) or more inclusive (1-2 Drop). If the drop envelope
is set incorrectly, recovery of the sorted population may be affected.
Figure 2.14 Drop Envelope
•
0.5 Drop
typically provides the poorest yield (most aborts). This is generally used when an exact sort
count is required such as for single cell deposition (Single Mode). It should never be used with
the Enrich Mode because positive events will be aborted.
• : One drop is sorted if the positive events fall anywhere within the drop. This envelope is
1 Drop
used when determining drop delay. Otherwise, the only time it should be used is when sorted
C37808AC
, and
Single Purify Enrich
Figure 2.14. Depending on the required sort application, researchers may
: One drop is sorted if all positive events are in the center half of the drop. This envelope
.
Using the CytExpert SRT Software
Sort Mode Library
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