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3.2 Automatic Continuous Measurement (6-cell auto mode)
Table 3-27 "Meas. PARAM." Setting Parameters
Setting Item
Data Mode
For estimating the purity of DNA, select 4) DNA.
[1] CONC : Used for generating a calibration curve
[2] ABS
[3] %T
[4] DNA
WL pair No.
Set up the number of pairs of wavelengths to be used
for measurement.
Select whether to perform ratio calculation for one
pair of wavelengths or two pairs of wavelengths.
[1] WL pair No. 1 : Selected for ratio calculation for
[2] WL pair No. 2 : Selected for ratio calculation for
Bkgd.
Specify whether to perform background correction.
CORR.
[1] ON : Selected when subtracting background
[2] OFF:
WL1 (nm) to
Enter a wavelength to be used for measurement.
WL4 (nm)
This parameter is settable in a range of 190.0 to
1100.0 nm in steps of 0.1 nm. The following
calculations are performed using the absorbance
values at the set wavelengths.
[WL pair No.: 1, Bkgd. CORR.: OFF]
Absorbance ratio = ABS (WL1)/ABS (WL2)
Absorbance difference = ABS (WL1) - ABS (WL2)
[WL pair No.: 1, Bkgd. CORR.: ON]
Absorbance ratio = (ABS (WL1) -
ABS (CORR. WL))/(ABS (WL2) - Data (CORR. WL))
Absorbance difference = ABS (WL1) - ABS (WL2)
[WL pair No.: 2, Bkgd. CORR.: OFF]
Absorbance ratio (WL: 1, 2) = ABS (WL1)/ABS (WL2)
Absorbance ratio (WL: 3, 4) = ABS (WL3) - ABS
(WL4)
[WL pair No.: 2, Bkgd. CORR.: ON]
Absorbance ratio (WL: 1, 2) = (ABS (WL1) -
ABS (CORR. WL))/(ABS (WL2) - ABS (CORR. WL))
Absorption ratio (WL: 3, 4) = (ABS (WL3) -
ABS (CORR. WL))/(ABS (WL4) - ABS (CORR. WL))
CORR. WL
Set a wavelength to be used for background
correction. This parameter is indicated only when
ON is selected for Bkgd. CORR.
The parameter is settable in a range of 190.0 nm to
1100.0 in steps of 0.1 nm.
3 - 62
Description
and determining the concentration.
(For details, refer to section 3.2.1.)
: Used for measuring the absorbance.
(For details, refer to section 3.2.2.)
: Used for measuring the transmittance.
(For details, refer to section 3.2.2.)
: Used for estimating the purity of DNA.
one pair (either A260/A280
(absorbance ratio between 260
nm and 280 nm) or A260/A230).
two pairs (both A260/A280 and
A260/A230).
absorption.
Selected when not subtracting
background absorption.
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