Hitachi U-5100 Instruction Manual page 128

3.2 Automatic Continuous Measurement (6-cell auto mode)
Bkgd.CORR
OFF Background correction is avoided. Absorbance ratio will be calculated using the
obtained absorbance values.
Absorbance ratio = A(1)/A(2)
ON The background will be corrected. This is effective when the background level is
even along the wavelength axis. The absorbance at the set correction wavelength
will be subtracted from those at the measuring wavelengths. Calculation is carried
out according to the following equation.
Absorbance ratio = (A(1) - A(correction))(A(2) - A(correction))
Explanation 3-8 Background Correction
A(1)
A(2)
WL2 WL1
Absorption spectrum
A(1)
A(2)
WL2 WL1
Absorption spectrum
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Setting method
Wavelength
A (correction)
Correction
wavelength
Wavelength