Hitachi U-5100 Instruction Manual page 69

Table 3-1 "Meas. PARAM." Setting Parameters
Setting Item
Data Mode For measuring the concentration of a solution, select
[1] CONC.
[1] CONC
[2] ABS :
[3] %T :
[4] DNA :
No. of WL Set the number of wavelengths to be used for
measurement. Normally specify "1". When
subtracting background absorption, specify "2" or "3".
(→ For details of setting, refer to Explanation 3-1.)
WL1 (nm) to Enter a wavelength to be used for measurement.
WL3 (nm) This parameter is settable in a range of 190.0 to
1100.0 nm in steps of 0.1 nm.
When "1" is set for No. of WL:
Enter a wavelength to be used for measurement for
WL1.
When "2" is set for No. of WL:
For WL1, enter a wavelength at which background
absorption is obtained. For WL2, enter a
wavelength at which absorption deriving from the
target substance for quantitative determination is
obtained. In so doing, it is required that WL1 > WL2.
When "3" is set for No. of WL:
For WL1 and WL3, enter a wavelength at which
background absorption is obtained. For WL3, enter
a wavelength at which absorption deriving from the
target substance for quantitative determination is
obtained. In so doing, it is required that WL1 >
WL2 > WL3.
Delay (s) After the [START] key is pressed, the system waits
for the time period set here and then starts
measurement. This parameter is settable in a range
of 0 to 9999 sec in steps of 1 sec.
Set this parameter when you want to start
measurement after a specified time period, including
when measuring a sample after its temperature
reaches the room temperature or to start
measurement after completion of reaction.
Enter "0" if the delay time need not be set.
3 - 5
Description
Used for measuring the absorbance.
(For details, refer to section 3.2.2..)
Used for measuring the transmittance.
(For details, refer to section 3.2.2..)
Used for estimating the purity of DNA.
(For details, refer to section 3.2.3..)
3.2.1