Figure 38. Safety Sleeve Connection To The Grounding Union (Esi Probe); Figure 39. Sample Tube Securely Tightened To The Grounding Union (Esi Probe) - Thermo Scientific LTQ Series Hardware Manual

Figure 38. Safety sleeve connection to the grounding union (ESI probe)

Figure 39. Sample tube securely tightened to the grounding union (ESI probe)

Tug on the fused-silica
sample tube to verify
that it is secured to the
grounding union.
Thermo Scientific
d. While holding the safety sleeve and sample tube firmly against the grounding union
receiving port, manually tighten the fitting as tight as you can
Figure 39).
e. Make sure that the sample tube is held tightly in the grounded union by gently
pulling it from the exit end of the ESI needle
8. Adjust the position of the sample tube as follows:
a. Using the fused-silica cutting tool, cut the sample tube approximately 2.5 cm (1 in.)
past the tip of the ESI needle.
b. From the ESI sample inlet, loosen the red PEEK fitting, and then pull the safety
sleeve backwards so that the sample tube is positioned appropriately within the ESI
needle (Figure 40).
The optimal position of the sample tube depends on the solvent flow rate, as follows:
• For flow rates less than or equal to 100 μL/min, set the sample tube protrusion to
1 mm past the ESI needle tip.
• For flow rates greater than 100 μL/min, make sure that the sample tube is flush
with the ESI needle tip or recessed inside the ESI needle by less than 1 mm
(Figure 41).
Natural PEEK ferrule
(Figure
5
Maintenance
Ion Source Probe Maintenance
(Figure 38 and
Grounding union
Two-wing, natural PEEK
fingertight fitting
39).
LTQ Series Hardware Manual 67