Figure 32. Front End Of The Esi Probe (Exploded View) - Thermo Scientific LTQ Series Hardware Manual

5
Maintenance
Ion Source Probe Maintenance
Assembling the ESI Probe
62 LTQ Series Hardware Manual
 To clean and dry the ESI manifold
IMPORTANT Always use LC/MS-grade methanol and LC/MS-grade water (see
Table 7 on page 55).
1. Disassemble the ESI probe (see steps
2. Rinse the ESI manifold with water and then with methanol.
3. Remove excess methanol from the manifold with a lint-free tissue.
4. Dry the component with nitrogen gas to make sure that all the solvent evaporates.
5. Inspect the spray needle, nozzle O-ring, HV socket O-ring, and needle seal.
6. Replace any damaged parts.
7. Reassemble the ESI probe (see the following procedure).
Follow this procedure after cleaning or replacing ESI probe components.
 To assemble the ESI probe
1. Insert the resistor and battery contact subassembly into the ESI manifold.

Figure 32. Front end of the ESI probe (exploded view)

Needle seal
ESI nozzle with
0.676 in. O-ring
2. Insert the entrance end of the ESI needle into the needle seal.
3. Position the ESI needle and needle seal in the ESI manifold.
4. Make sure that the 0.676 in. ID O-ring is in the precut groove on the ESI nozzle
(Figure 31 on page 61).
5. Thread the ESI nozzle over the needle and into the ESI manifold. Slightly wet the nozzle
threads with LC/MS-grade methanol for lubrication.
3–5 in "To disassemble the ESI probe"
Resistor
Battery contact
26-gauge needle
on page
ESI manifold
Thermo Scientific
59).