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Summary of Contents for Thermo Scientific LH-4000W
- Page 1 Thermo Scientific LH-4000W Instruction Manual 50134636-b • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
- Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
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Page 3: Table Of Contents
Chapter 6 Maintenance and Care ......................6-1 Cleaning Intervals ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-5 RCF-Values .......................A-1 Chemical Compatibility Chart ................B-1 Thermo Scientific 75006476 LH-4000W... -
Page 5: Preface
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty. Scope of Supply Article Number Quantity Check 75006476 LH-4000W with Bucket 76003500 Grease for rubber seal 75003786 Bolt grease 50134706 CD with manual If any parts are missing, please contact your nearest Thermo Fisher Scientific representative. - Page 6 Preface In order to ensure safe operation of the LH-4000W rotor, the following general safety regulations must be followed: • Do not remove the magnet at the rotor bottom. • Do not use rotors which show any signs of corrosion and/or cracks.
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Page 7: Rotor Data
Rotor Data Contents “Technical Data” page 1-2 • Thermo Scientific 75006476 LH-4000W... -
Page 8: Technical Data
Max. Speed n [ rpm ] at 4 °C 4400 4400 4400 yes (with cap 75006479) yes (with cap 75006479) yes (with cap 75006479) Aerosol-tight Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg 75006476 LH-4000W Thermo Scientific... - Page 9 Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 yes (with cap 75006421) Aerosol-tight Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg Thermo Scientific 75006476 LH-4000W...
- Page 10 Acceleration / Braking Time [ s ] 110 / 90 Sample Heating at n [ °C ] referred to Ambient Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 Aerosol-tight Autoclavable 75006476 LH-4000W Thermo Scientific...
- Page 11 Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 yes (with cap 75006479) Aerosol-tight Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg Thermo Scientific 75006476 LH-4000W...
- Page 12 Acceleration / Braking Time [ s ] 110 / 90 Sample Heating at n [ °C ] referred to Ambient Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 Aerosol-tight Autoclavable 75006476 LH-4000W Thermo Scientific...
- Page 13 Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 4400 yes (with cap 75006479) yes (with cap 75006479) Aerosol-tight Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg Thermo Scientific 75006476 LH-4000W...
- Page 14 Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 yes (with cap 75006421) Aerosol-tight Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg 75006476 LH-4000W Thermo Scientific...
- Page 15 Acceleration / Braking Time [ s ] 110 / 90 Sample Heating at n [ °C ] referred to Ambient Temperature of 23-25 °C, Run Time 60 Minutes 4400 Max. Speed n [ rpm ] at 4 °C Aerosol-tight Autoclavable Thermo Scientific 75006476 LH-4000W...
- Page 16 Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4400 Aerosol-tight yes (with cap 75006479) Permissible temperature range autoclavable °C tested by TÜV Product Service GmbH - Hamburg 1-10 75006476 LH-4000W Thermo Scientific...
- Page 17 Acceleration / Braking Time [ s ] 85 / 85 Sample Heating at n [ °C ] referred to Ambient Temperature of 23-25 °C, Run Time 60 Minutes Max. Speed n [ rpm ] at 4 °C 4100 Aerosol-tight Autoclavable Thermo Scientific 75006476 LH-4000W 1-11...
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Page 19: Accessories
Accessories Contents “Rotor data” • page 2-2 “Accessories” page 2-2 • Thermo Scientific 75006476 LH-4000W... -
Page 20: Round Bucket 75006477
2 x 35 and 65 g each Tare plates 75005759 Tare weights for plastic insert 1 set of 4 weights, 6 g and 15 g each 75007645 75006485 Max. Dimensions with aerosol-tight cap An additional pad obtainable from tube manufacturer is necessary. 75006476 LH-4000W Thermo Scientific... -
Page 21: Double Rectangular Bucket Doublespin 75006478
76007677 Tare weight Tare range 208-1700 g 75007668 Tare weights for plastic insert 75007647 1 set of weights, 6 g and 15 g each 75007645 Caoutchouc 2 x 35 and 65 g each Tare weight 75005759 Thermo Scientific 75006476 LH-4000W... -
Page 23: Rotor Installation
Rotor Installation Contents “Rotor Installation” page 3-2 • “Removing the Rotor” page 3-2 • Thermo Scientific 75006476 LH-4000W... -
Page 24: Removing The Rotor
2. Thread the fastening tool in the centrifuge spindle counterclockwise. Hold the rotor with the other hand into position. 3. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to tilt the rotor while doing this. 75006476 LH-4000W Thermo Scientific... -
Page 25: Rotor Loading
Rotor Loading Contents “Before a Run” page 4-2 • “Proper Loading” page 4-2 • “Improper Loading” page 4-3 • “Maximum Loading” page 4-3 • “Cycle Counter” page 4-4 • Thermo Scientific 75006476 LH-4000W... -
Page 26: Before A Run
Weigh the bucket content (adapter and tube). Make sure you do not exceed the maximum compartment load. CAUTION Always be sure to use buckets of the same weight class. Proper Loading To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. 75006476 LH-4000W Thermo Scientific... -
Page 27: Improper Loading
• Reduce the fill level. • Reduce the speed. Use the table or the formula: Actual Load maximum speed 6000 5902 5809 5721 5636 5555 5477 5403 5331 5262 5196 5132 5071 5012 Thermo Scientific 75006476 LH-4000W... -
Page 28: Cycle Counter
220 days / year Usage profile Maximum service life at 20,000 cycles frequent use 3 years 30 runs / day 220 days / year average use 9 years 10 runs / day 220 days / year 75006476 LH-4000W Thermo Scientific... - Page 29 Aerosol-tight Applications Contents “Basic Principles” page 5-2 • “Closing aerosol-tight” page 5-2 • “Fill Level” page 5-3 • “Checking the Aerosol-Tightness” page 5-3 • Thermo Scientific 75006476 LH-4000W...
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Page 30: Chapter 5 Aerosol-Tight Applications
CAUTION If the levers are not flapped down properly can damage the bucket during centrifugation. The bucket is not closed aerosol-tight if the levers are not in their locking position. Never lift the bucket on the levers. 75006476 LH-4000W Thermo Scientific... -
Page 31: Fill Level
The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement is extremely important. Aerosol-tight applications are not possible if the lids are open. Aerosol-tightness requires the correct operation when filling the sample vessels and closing the bucket cap. Thermo Scientific 75006476 LH-4000W... - Page 32 Lubricate all seals lightly. Damaged seals must be replaced immediately. When loading the rotor, ensure that the rotor lid closes securely. Damaged or clouded rotor caps must be replaced immediately. 75006476 LH-4000W Thermo Scientific...
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Page 33: Maintenance And Care
Maintenance and Care Contents “Cleaning Intervals” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-5 Thermo Scientific 75006476 LH-4000W... -
Page 34: Cleaning Intervals
Clean centrifuge and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. 75006476 LH-4000W Thermo Scientific... -
Page 35: Disinfection
Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. Thermo Scientific 75006476 LH-4000W... -
Page 36: Decontamination
4. Remove the buckets from the rotor. 5. Remove the centrifuge tubes and adapters from the bucket and decontaminate them. 6. Release the rotor. 7. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle. 75006476 LH-4000W Thermo Scientific... -
Page 37: Autoclaving
Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician. The service technician checks the following: • the electrical equipment; • the suitability of the set-up site; Thermo Scientific 75006476 LH-4000W... - Page 38 Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid if the centrifuge has only been maintained by a Thermo Fisher Scientific service technician. 75006476 LH-4000W Thermo Scientific...
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Page 39: Rcf-Values
1700 24.7 1800 24.7 1900 24.7 2000 24.7 1105 2100 24.7 1218 2200 24.7 1337 2300 24.7 1461 2400 24.7 1591 2500 24.7 1726 2600 24.7 1867 2700 24.7 2013 2800 24.7 2165 2900 24.7 2322 Thermo Scientific 75006476 LH-4000W... - Page 40 11.5 21.5 11.5 21.5 11.5 21.5 1000 11.5 21.5 1100 11.5 21.5 1200 11.5 21.5 1300 11.5 21.5 1400 11.5 21.5 1500 11.5 21.5 1600 11.5 21.5 1700 11.5 21.5 1800 11.5 21.5 1900 11.5 21.5 75006476 LH-4000W Thermo Scientific...
- Page 41 11.5 21.5 2161 4041 4200 11.5 21.5 2268 4240 4300 11.5 21.5 2377 4444 4400 11.5 21.5 2489 4654 Double Rectangular Bucket 75006436 Table A-3. RCF R RCF R 25.3 25.3 25.3 25.3 25.3 25.3 25.3 Thermo Scientific 75006476 LH-4000W...
- Page 42 3600 25.3 1594 3666 3700 25.3 1684 3872 3800 25.3 1776 4084 3900 25.3 1871 4302 4000 25.3 1968 4526 4100 25.3 2067 4755 4200 25.3 2169 4990 4300 25.3 2274 5230 4400 25.3 2381 5476 75006476 LH-4000W Thermo Scientific...
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Page 43: Chemical Compatibility Chart
U S S M S S U S S S S S S S S S U S S S S S S S S S S S S S - S S S S - S S - S S S S S S M S S S Cesium Acetate Thermo Scientific 75006476 LH-4000W... - Page 44 U U U U S U U U S S M M S U U U - Hydrochloric Acid (conc.) U U U U - U U M - U M U U M U U U - U S U U U - 75006476 LH-4000W Thermo Scientific...
- Page 45 M S M - S S S S S S S M S M S S - N-Butyl Alcohol S U - S M - U S M S S S S M M S M - Thermo Scientific 75006476 LH-4000W...
- Page 46 S S S U S S S Nitric Acid (10 %) U S U S S U U - S U S U - S S S S S S S S S M S S S S 75006476 LH-4000W Thermo Scientific...
- Page 47 Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc. Suggest testing under actual conditions of use. Unsatisfactory, not recommended. Performance unknown; suggest testing, using sample to avoid loss of valuable material. Thermo Scientific 75006476 LH-4000W...
- Page 48 Chemical Compatibility Chart Chemical resistance data is included only as a guide to product use. No organized chemical resistance data exists for materials under the stress of centrifugation. When in doubt we recommend pretesting sample lots. 75006476 LH-4000W Thermo Scientific...
- Page 49 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2012-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.
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