Solvents; Solvents With Low Boiling Points; Buffers - PerkinElmer 200 UV/VIS Series User Manual

Safety and Regulatory Information

Solvents

• Always use clean solvents. Solvents which have been distilled in glass (HPLC Grade) are
recommended.
• Filter the solvents and buffers through a 0.5-micron medium as an additional precau-
tion.
• Degas all aqueous and most organic solvents prior to use.
• Store flammable solvents or solvents which may form hazardous by-products when the
instrument is shut down, by following the recommended shutdown procedure.
• Check compatibility of solvent(s) with the type of column(s) being used.
• Know the relative polarity and miscibility of the solvents being used.

Solvents with Low Boiling Points

Do not use liquids that have a boiling point less than 30 °C.

Buffers

Exercise care when using buffers in conjunction with organic solvents. NEVER LEAVE
BUFFERS IN THE SYSTEM OVERNIGHT. Buffers left in the system can form salt crystals
which may cause premature injector valve failure and plug the transfer tubing and sample
needle. To remove buffers, flush the system with water followed by methanol or isopropanol.
Remember to change the flush solvent from methanol or isopropanol to water before using
buffers.
S - 16
Do not use carbon disulfide or other solvents
which have an auto-ignition temperature
below 110 °C.
Safety and Regulatory Information