Nitrogen Purge; Detector Flowcell - PerkinElmer 200 UV/VIS Series User Manual

Safety and Regulatory Information

Nitrogen Purge

In some cases (for example, in cold rooms or when operating at wavelengths of 200 nm or
lower), it is recommended that you purge the monochromator (via the purge inlet) with puri-
fied nitrogen during use. The flow of nitrogen gas will help reduce or minimize thermal
expansion/contraction of the monochromator, and, in cold rooms, prevent moisture from con-
densing inside the monochromator housing. Use only connecting tubing that is free of soften-
ers or placticizers. Purge with nitrogen at approximately 1-2 psi. (3 psi maximum). Allow 10
to 12 minutes for the monochromator to become completely filled. Continue this nitrogen
flow while the instrument is in use. Purging is particularly important when the instrument
is removed from a cold room and placed in a high-humidity room at ambient temperature.

Detector Flowcell

The standard 12 µL flowcell has a normal flow of 1 mL/min of 50/50 ACN/H
tional information, refer to Chapter 7, Maintenance for the specific procedures on flowcell
maintenance.
S - 10
If buffers are being used, flush the flowcell
with water to remove all buffers prior to
maintenance. Buffer may precipitate and
clog the flowcell.
Flowcell backpressure should not exceed 500
psi. Damage to the flowcell and detector
occurs at higher pressures.
Do not overtighten the flowcell connections.
Damage to the flowcell could result.
Safety and Regulatory Information
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