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PerkinElmer 200 UV/VIS Series User Manual

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Series 200 UV/VIS Detector
User's Manual

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Summary of Contents for PerkinElmer 200 UV/VIS Series

  • Page 1 Series 200 UV/VIS Detector User’s Manual...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Safety And Regulatory Information

    Electromagnetic Compatibility (EMC) Safety and Regulatory Information Safety and Regulatory Information Electromagnetic Compatibility (EMC) United States This product is classified as a digital device used exclusively as industrial, commercial, or medical test equipment. It is exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations, based on Section 15.103 (c).

  • Page 4: Symbols And Conventions Used In This Manual

    Safety and Regulatory Information Safety and Regulatory Information Your Series 200 UV/VIS Detector was manufactured and tested at the PerkinElmer facility in Shelton, Connecticut. The following Safety and Regulatory Information is provided for your information and should be read before proceeding to the installation section.

  • Page 5: Symbols Used On The Instrument

    Safety and Regulatory Information Safety and Regulatory Information Symbols Used on the Instrument There are two different types of warning symbols that appear on the instrument. This symbol indicates an operation (or condi- tion on the instrument) that could cause per- sonal injury if precautions are not followed.

  • Page 6: Warnings/Labels On The Instrument (Label Location On Rear Panel

    Safety and Regulatory Information Safety and Regulatory Information Warnings/Labels on the Instrument (Label Location on Rear Panel) Figure S-2. Location of labels on the Series 200 UV/VIS rear panel. Label No. Explanation (in photo) Safety information Instrument name, part number, serial number, CE mark Fuse, voltage, frequency and power information Do not restrict air intake or exhaust Attention: N’obstruez pas l’arrivée ou l’évacuation de l’air.

  • Page 7: Warning For Front Panel

    Safety and Regulatory Information Safety and Regulatory Information Warning for Front Panel You may need to open the front panel to replace a lamp. Chapter 7, Maintenance, explains this procedure. The following illustration contains safety information for the procedure. Allen bolt and Allen wrench Location of label used in lamp replacement procedure.

  • Page 8: Electrical Warnings - General

    Do not attempt to make adjustments, replacements or repairs to this instrument except as described in the accompanying User Documentation. Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument.
  • Page 9 Observe the following guidelines for electrical safety: • Service on electrical components should be performed only by a qualified PerkinElmer service representative or similarly trained and authorized person. • Always turn off the all components to the Series 200 UV/VIS Detector and disconnect the power cord from the electrical outlet before you remove any covers.

  • Page 10: Environmental Considerations

    Safety and Regulatory Information Safety and Regulatory Information Environmental Considerations The Series 200 UV/VIS Detector was designed for indoor use and will function correctly under the following ambient conditions: Temperature 10 – 35 °C Relative Humidity 20 – 80% noncondensing Altitude 0 –...

  • Page 11: Internal Warning Labels

    If the detector has been operated recently, the lamp assembly will be very hot. Check carefully before touching to avoid personal injury. Replace lamp with PerkinElmer part number N292-0149. NITROGEN Do not exceed 3 PSI for Nitrogen input INPUT 3 PSI MAX.

  • Page 12: Nitrogen Purge

    Safety and Regulatory Information Safety and Regulatory Information Nitrogen Purge In some cases (for example, in cold rooms or when operating at wavelengths of 200 nm or lower), it is recommended that you purge the monochromator (via the purge inlet) with puri- fied nitrogen during use.

  • Page 13: Storage Conditions

    Observe the following guidelines: • Service on electrical components should be performed only by a qualified PerkinElmer service representative or similarly trained and authorized person. • Be sure the power cord is the correct one for your laboratory. The line cord used must meet the national safety agency’s guidelines for the particular country of use.
  • Page 14 Fuses Note Service on electrical components should be performed only by a qualified PerkinElmer service representative or similarly trained and authorized person The fuses in the power entry module can be changed by the user. The following fuses are in the power entry module to protect the instrument: 100, 120, 220, 230/240 VAC at 50/60 Hz: 1.6 Amp, Type T (H - high interrupting capacity), 5...

  • Page 15: Quality Control/Good Laboratory Practices

    To maintain functional performance, PerkinElmer recommends a yearly Instrument Perfor- mance Verification (IPV) of the Series 200 UV/VIS Detector and its other system compo- nents by a PerkinElmer Service Engineer to ensure its operation within published specifications. These tests consist of measuring the most important UV/VIS characteristics such as noise drift and wavelength accuracy.

  • Page 16: Definitions In Warning For Hazardous Chemicals

    Safety and Regulatory Information Safety and Regulatory Information Some chemicals used with this instrument may be hazardous or may become hazardous after completion of an analysis. The responsi- ble body (e.g., Lab Manager) must take the necessary precautions to ensure that the sur- rounding workplace and instrument opera- tors are not exposed to hazardous levels of toxic substances (chemical or biological) as...

  • Page 17: Series 200 Operating Precautions

    Safety and Regulatory Information Safety and Regulatory Information Series 200 Operating Precautions To protect yourself from harm and to prevent malfunctioning of the system, please observe the following guidelines when operating the UV/VIS Detector: • Before using the instrument, pay attention to the warnings presented in the beginning of the manual.

  • Page 18: Solvents

    Safety and Regulatory Information Safety and Regulatory Information Solvents • Always use clean solvents. Solvents which have been distilled in glass (HPLC Grade) are recommended. • Filter the solvents and buffers through a 0.5-micron medium as an additional precau- tion. •...

  • Page 19: Corrosion

    All parts of the Series 200 UV/VIS Detector that contact mobile phase are made of stainless steel, KelF, and quartz. Some of these materials are extremely sensitive to acid chlorides. If you have questions about your mobile phase or flush solvent, contact a PerkinElmer repre- sentative. Refer to the table below.

  • Page 20: Solvents With Auto-Ignition Temperature Below 110 °C

    Safety and Regulatory Information Safety and Regulatory Information Solvents with Auto-Ignition Temperature Below 110 °C Certain solvents have a temperature at which they are combustible upon contact with a heated surface, even in the absence of a spark or other source of ignition. A list of common solvents with their auto-ignition temperatures are in Table S-3.

  • Page 21: Pressure Buildup

    Safety and Regulatory Information Safety and Regulatory Information Pressure Buildup Over time, you may observe a gradual increase in the system operating pressure. If you observe pressure readings greater than 3.45 MPa (500 psi) above the normal operating pres- sure of your analysis, the following items may need attention: •...

  • Page 22: Warnings From The User's Manual

    Safety and Regulatory Information Warnings from the User’s Manual Warnings from the User’s Manual Generic Warnings Before installing or operating the Detector, read the following information concerning haz- ards and potential hazards. You should ensure that anyone involved with installation and/or operation of the instrument is knowledgeable in both general safety practices for the labora- tory and safety practices for the.
  • Page 23 Safety and Regulatory Information Warnings from the User’s Manual S - 21...
  • Page 24 Safety and Regulatory Information Warnings from the User’s Manual S - 22...

  • Page 25: Table Of Contents

    Table of Contents . Safety and Regulatory Information ..........S-1 Electromagnetic Compatibility (EMC) .
  • Page 26 Table of Contents Getting Started with the Detector ......1-1 Series 200 UV/VIS LC Detector Overview ......1-2 Series 200 UV/VIS LC Detector Features and Benefits .
  • Page 27 Table of Contents Using Method Mode ........4-1 Understanding Methods .
  • Page 28 Table of Contents Photodiode and Monochromator Windows ......7-8 Lamp Replacement ......... . . 7-11 Troubleshooting Detector Problems .

  • Page 29: Getting Started With The Detector

    Getting Started with the Detector This chapter introduces you to the Series 200 UV/VIS LC Detector and includes the follow- ing information. • A Description of the Series 200 UV/VIS LC Detector • The Series 200 UV/VIS LC Detector’s Features and Benefits •...

  • Page 30: Series 200 Uv/Vis Lc Detector Overview

    Figure 1-1. The Series 200 UV/VIS LC Detector The PerkinElmer Series 200 UV/VIS LC Detector can be used with virtually every liquid chromatography system or application. You configure the Detector and define all of your methods and operations using function keys on a keyboard located on the front panel of the instrument.

  • Page 31: Series 200 Uv/Vis Lc Detector Features And Benefits

    Series 200 UV/VIS LC Detector Features and Benefits Getting Started with the Detector Series 200 UV/VIS LC Detector Features and Benefits • Compatibility with the common LC platform. • Ultraviolet or visible lamp capability. • Graphical display of chromatogram on module display. •...

  • Page 32: Main Components Of The Series 200 Uv/Vis Lc

    Getting Started with the Detector Main Components of the Series 200 UV/VIS LC Detector Main Components of the Series 200 UV/VIS LC Detector The Series 200 UV/VIS LC Detector consists of the following main components. A brief description of each of the components follows. Refer to Figure 1-2 for the location of the following components.
  • Page 33 Main Components of the Series 200 UV/VIS LC Detector Getting Started with the Detector Access Door Keyboard and Display Input/Output Connector Panel Figure 1-2. Front Right Side View of UV/VIS Detector 1 - 5...

  • Page 34: Rear Panel

    Getting Started with the Detector Main Components of the Series 200 UV/VIS LC Detector Power Input Module Knurled knob Serial Ports Figure 1-3. Rear Panel of the UV/VIS Detector. Rear Panel The rear panel contains the Power Input Module and the serial ports for the UV/VIS Detector.

  • Page 35: Powering Up The Detector

    Powering Up the Detector Getting Started with the Detector Powering Up the Detector Press the power switch located on the rear panel of the Detector to the ON position. The Copyright and Self Test Screen first appears on the Liquid Crystal Display. The display is located directly above the keyboard on the front panel.

  • Page 36: Understanding The Main Screen

    Getting Started with the Detector Powering Up the Detector Understanding the Main Screen The Main Screen provides access to all of the Detector’s functions. The soft keys displayed along the bottom of the default Main Screen correspond to the functions that are available when you first power up the Detector.

  • Page 37: Using The Keyboard And Display

    Using the Keyboard and Display Getting Started with the Detector Using the Keyboard and Display The UV/VIS Detector keyboard and Liquid Crystal Display (LCD) are located on the front panel of the instrument as shown in the figure below. You configure the UV/VIS Detector and define all of your methods by filling in information on the screens that appear in the LCD.

  • Page 38: Summary Of The Keyboard Functions

    Getting Started with the Detector Using the Keyboard and Display Summary of the Keyboard Functions Table 1-1. Summary of Keyboard Functions. Function Function Keys Press a function key to select the corresponding screen-defined soft key label displayed above it. For example, press F7 from the Main Screen CNFG to go to the Configure Screen.
  • Page 39 Using the Keyboard and Display Getting Started with the Detector Function Edit Keys Press to insert a duplicate of the current step below the current step and insert increment the steps following it by one (for example, when adding methods in a sequence).
  • Page 40 Getting Started with the Detector Using the Keyboard and Display Screen Hierarchy Diagram The following figure provides an overview of the hierarchy of the detector’s screens. Figure 1-5. Screen Hierarchy Diagram 1 - 12...

  • Page 41: Keyboard Operations

    Using the Keyboard and Display Getting Started with the Detector Keyboard Operations This section provides an overview of how to perform the following basic operations using the LC Detector keyboard: • Selecting screen options • Selecting data fields and entering numeric information •...
  • Page 42 Getting Started with the Detector Using the Keyboard and Display Selecting Screen Options Use the Function Keys: To select options displayed on a screen, either as screen-defined soft keys, or as a list of items, use the appropriate function key. Once you press the appropriate function key, the screen corresponding to the selected option appears.
  • Page 43 Using the Keyboard and Display Getting Started with the Detector Selecting Data Fields and Entering Numeric Information Use the cursor arrow keys, and the numeric keys To accept the entry in a highlighted data field and move to the next field, use the cursor arrow keys, or press the Enter key.
  • Page 44 Getting Started with the Detector Using the Keyboard and Display Entering Non-numeric Information into Data Fields within a Screen Use the Function Keys: If a highlighted data field requires a non-numeric entry, the options for that field appear as screen-defined soft keys. To change the current entry in the highlighted field, press the corresponding function key (F1 through F8).
  • Page 45 Using the Keyboard and Display Getting Started with the Detector Scrolling Through Screen Lists Use the cursor arrow keys, If a list of items is displayed on a screen, use the cursor arrow keys to move the list up and down.
  • Page 46 Getting Started with the Detector Using the Keyboard and Display Responding to Screen Prompts To respond to a Y/N prompt appearing in the Message line of a screen, such as the one shown in the following screen, press the numeric key to select Y and the to select N.
  • Page 47 Using the Keyboard and Display Getting Started with the Detector Saving Changes to Screens When you finish making changes to a screen, press the key to save those changes. return Changes you make to a method screen will remain in effect only until you turn off the power to the Detector.

  • Page 48: Instrument Specifications

    Getting Started with the Detector Instrument Specifications Instrument Specifications Table 1-2. Specifications. Specification Description Technical Specifications Wavelength range 190-700 nm Bandwidth 5 nm Wavelength accuracy +/- 1 nm Optics Dual beam Sensitivity Range 0.0005 to 3.000 AUFS in 0.0001 increments from 0.0005 to 0.1, and 0.01 increments above 0.1 AUFS.
  • Page 49 Instrument Specifications Getting Started with the Detector Specification Description Control outputs 4 SPDT relays, 1 A @ 48 V (max); Relay TE 2 is used to control an external recorder for scanning. RUN OUT: momentary (approximately 2 seconds) contact clo- sure the instant a method starts.
  • Page 50 Getting Started with the Detector Instrument Specifications 1 - 22...

  • Page 51: Installation

    Installation This chapter describes how to install your Series 200 UV/VIS LC Detector. It includes the following information: • Preparing your laboratory • Unpacking your detector • Making the electrical connections • Making plumbing connections...

  • Page 52: Preparing Your Laboratory

    Installation Preparing Your Laboratory Preparing Your Laboratory Before installing your Series 200 UV/VIS LC Detector, prepare your laboratory according to the following guidelines: Required Air Quality To minimize contamination problems in your laboratory, provide a relatively dust-free envi- ronment. Make sure that the following gases or vapors are not present at levels exceeding federal, state, and local ordinances for continuous human exposure: •...
  • Page 53 Preparing Your Laboratory Installation To prevent degradation of the detector’s performance, the AC line voltage must remain within ±10% of the nominal specified voltage (displayed on the label over the line cord). If there are large voltage fluctuations, install a voltage regulator between the detector and the AC outlet.

  • Page 54: Unpacking Your Detector

    Start-Up Kit parts. The part numbers should help you to identify items in the kit, but do not use these part numbers to order replacements. You can order replacement parts from PerkinElmer’s catalog service as described on the Customer Service page of this manual.

  • Page 55: Making Electrical Connections

    Making Electrical Connections Installation Making Electrical Connections The Detector is shipped from the factory with: Caution • The operating voltage set to 120 VAC. • The main AC power switch set to the OFF position. You need to select the appropriate voltage set- ting and install the proper fuse(s) before install- ing the line cord and applying AC power.
  • Page 56 Installation Making Electrical Connections To set the Series 200 UV/VIS LC Detector’s operating voltage: 1. Make sure the main power switch is in the OFF position. Remove the power cord from the module. 2. Refer to Figure 2-1. Remove the power input module cover by inserting a small flat blade screwdriver into the notch and carefully pry off the cover.

  • Page 57: Installing The Ac Line Cord

    Making Electrical Connections Installation 6. After setting the voltage range, install the fuse. See Figure 2-2 for the location of the fuse block. Slide the fuse into the fuse holder. Figure 2-2. Fuse Location Installing the AC Line Cord Warning Ground circuit continuity is vital for safe operation of equipment.
  • Page 58 Installation Making Electrical Connections 1. Select the proper line cord for your location. 2. Ensure that the power switch is off and insert the line cord plug into the AC supply. O ld B ritish S tandard N orth A m erica B S 546 N E M A -5-15 India...

  • Page 59: Location Of Electrical Connections

    Making Electrical Connections Installation Location of Electrical Connections Electrical connections are located on the rear panel or interior as described in the following table. Table 2-3. Electrical Connections, Their Functions, and Location Control Function Location Power Connector Universal male AC line Rear Panel connector for power cord input;...

  • Page 60: Recorder And Computer/Integrator Connections

    Installation Making Electrical Connections Recorder and Computer/Integrator Connections The recorder and computer analog output connectors are located on the right panel. (See Figure 2-3 on the next page.) Cables are supplied with the Series 200 UV/VIS LC Detector for both connectors. The REC connector has a 10 mV/FS analog signal output and should be connected with a chart recorder with the same range for optimal performance.

  • Page 61: Making Input/Output Connections

    Making Input/Output Connections Installation Making Input/Output Connections The input/output connections on the Series 200 UV/VIS LC Detector uses phone jack connec- tors for quick contact closure connections between instruments. Figure 2-3 shows the input/ output connectors on the right-side panel of the Series 200 UV/VIS LC Detector. Table 2-4 provides a description of each input/output connector.

  • Page 62: Making A Connection Between Modular Cable And Twisted Pair Wires

    Installation Making Input/Output Connections Description Input\Output Connector This contact closure has two modes of operation. The first mode is identical to TE1. The second mode of operation is associated with Scanning. If this mode is chosen, when the detector is scanning, this contact will close for the duration of the scan.
  • Page 63 Making Input/Output Connections Installation Figure 2-5. Connecting a Wire to the Modular Connector. 2 - 13...
  • Page 64 Installation Making Input/Output Connections Figure 2-6. System Connection Diagram: Series 200 LC Autosampler, Series 200 LC Pump, Series 200 UV/VIS Detector, and a Data System. 2 - 14...

  • Page 65: Making Tubing Connections

    The Start up Kit contains a supply of Fingertight PEEK fittings (PN 0092-0513) for connect- ing to the Detector inlet and outlet. All PerkinElmer LC systems utilize connections made with stainless steel nuts, ferrules, and tubing are rated to 6000 psi.
  • Page 66 Installation Making Tubing Connections Blade Edge or Triangular File Scribe Line Smooth Jaw Pliers Figure 2-7. Cutting the .010-inch Tubing. 2 - 16...

  • Page 67: To Make The Tubing Connections

    Making Tubing Connections Installation Caution Use care when making fluid connections. Over-tightened fittings may crimp tubing and cause flow stoppage or excessive backpres- sure while under-tightened fittings may cause solvent leaks around the fitting. Damage caused by overtightening or undertightening connections is not covered under warranty.
  • Page 68 Installation Making Tubing Connections Waste OUT Column Effluent IN Figure 2-8. Tubing Connection Locations Table 2-5. Plumbing Connections and Their Functions Connection Function Sample In Connector Bulkhead union (1/l6-inch OD, 0.010-inch ID) for connection of column outlet tubing to heat exchanger inlet.

  • Page 69: Making External Tubing Connections

    Parker-Hannifin Zero- This union combines two pieces of 1/16- union Dead-Volume Union, inch tubing for a zero-dead-volume union. PerkinElmer Part No. This type of union makes it easy for you 0990-3289 to disconnect the Autosampler from the pump. Scavanger Column...
  • Page 70 Installation Making Tubing Connections Figure 2-8 provides an overview of typical tubing connections between the Detector and other instruments making up an LC system. Figure 2-9. Typical System Tubing Connections 2 - 20...

  • Page 71: Using Manual Mode

    Using Manual Mode This chapter describes how to use the detector’s Manual mode of operation. It includes the following topics. • Understanding Manual mode • Viewing the Detector Manual Settings Screen • Setting control parameters • Scanning the chromatogram...

  • Page 72: Understanding Manual Mode

    Using Manual Mode Understanding Manual Mode Understanding Manual Mode When you set the detector in the Manual Mode, the default main screen is the Detector Man- ual Settings Screen shown below. In Manual mode, you can manually set, and thereby immediately change, the operating parameters for the detector.

  • Page 73: Viewing The Detector Manual Settings Screen

    Viewing the Detector Manual Settings Screen Using Manual Mode Viewing the Detector Manual Settings Screen To set the Detector to manual or method operation, press the CNFG softkey. The Detector displays the Configure menu shown below. To switch to manual operation, from the Configure menu press the MAN softkey. The Detec- tor displays the following message: Select to make the change.

  • Page 74: The Parts Of The Detector Manual Settings Screen

    Using Manual Mode Viewing the Detector Manual Settings Screen The Parts of the Detector Manual Settings Screen The following figure identifies the parts of the Detector Manual Settings Screen. The following table describes the parts of the Detector Manual Settings Screen. Part of the Number Description...
  • Page 75 Viewing the Detector Manual Settings Screen Using Manual Mode Part of the Number Description Screen CNFG Softkey Use to display the Configure menu options. For more information on configuring the detector, see Chapter 6, Configuring the Detector. SCAN Softkey Use to perform a stop flow scan. See the “Scanning the Chromatogram”...

  • Page 76: Setting Operating Parameters For Immediate Change

    Using Manual Mode Setting Operating Parameters for Immediate Change Setting Operating Parameters for Immediate Change In Manual mode, you can change the detector’s operating parameters at any time. Setting a parameter on the Manual mode screen takes effect as soon as you press the Enter key to move to another field.
  • Page 77 Setting Operating Parameters for Immediate Change Using Manual Mode Press the right arrow key or the key to move to the RSP/PW field. Depending enter on the detector’s configuration, either RSP or PW is displayed for this field. You use this field to select a value for the response time or peak width at half height.

  • Page 78: Scanning The Chromatogram

    Using Manual Mode Scanning the Chromatogram Scanning the Chromatogram Scanning lets you scan the sample in the flowcell across the selected wavelength range after stopping the flow. Scanning allows you to create, on the recorder, a spectrum of the sample trapped in the flowcell.
  • Page 79 Scanning the Chromatogram Using Manual Mode Parameter Possible Values λ Depends on the Configuration options that have been set. For Enter the wavelength where the example: scan should begin. Deuterium Lamp: 190 to 360 nm Tungsten Lamp: 360 to 700 nm λ...
  • Page 80 Using Manual Mode Scanning the Chromatogram To start the scan, press F8 . The Detector displays the SCAN IN PROGRESS STRT Screen. When the scan is finished, the detector turns off the recorder, if controlled from TE2, and returns you to the Scan screen. Press the key to return to the Detector Manual Settings Screen.

  • Page 81: Using Method Mode

    Using Method Mode This chapter describes how to create Methods on your Series 200 UV/VIS LC Detector. It includes the following information: • Understanding Methods • Viewing the Method Screen • Editing the Current Method • Creating a New Method •...

  • Page 82: Understanding Methods

    Using Method Mode Understanding Methods Understanding Methods When you set the detector to Method Mode, the default main screen is the Method Screen. The first time you start the detector in Method Mode, the detector displays the default method. This method has no method number or name, and it is not stored, as shown in the figure below.

  • Page 83: Viewing The Method Screen

    Viewing the Method Screen Using Method Mode Viewing the Method Screen Depending on instrument configuration previously selected, the Detector either displays the Manual Operation or Method Operation Screen. To set the Detector to Method operation, press the CNFG softkey. The Detector displays the Configure menu shown below. CONFIGURE MENU To switch from manual to method operation, press F6 .

  • Page 84: The Parts Of The Method Status Screen

    Using Method Mode Viewing the Method Screen The Parts of the Method Status Screen The following figure identifies the method status, and some parts of the Method Screen. The following table describes the parts of the Method Screen. Part of the Number Description Screen...
  • Page 85 Viewing the Method Screen Using Method Mode Part of the Number Description Screen Current Displays the current values for the detector’s operating Control parameters. For more information see the “Editing the Parameters Current Method” topic later in this chapter. Elapsed Time Indicates the time in minutes that has elapsed since the start of a run.

  • Page 86: Displaying The Current Method

    Using Method Mode Displaying the Current Method Displaying the Current Method When you switch the Detector to Method mode, the current method is the method that appears on the display. The current method is the method that is controlling the detector. A method contains two parts: control parameters and timed events.
  • Page 87 Editing the Current Method Using Method Mode The following table describes the Control Parameter values: Value Description STEP Displays the step number. A method may have up to 20 steps. TIME Indicates the length of time in which the selected control parame- ters will be in control of the detector.
  • Page 88 Using Method Mode Editing the Current Method To edit the control parameters, follow the procedure below. From the Method Screen, press F2 to display the Method Edit Screen. This EDIT screen displays the Steps in the method. The caret highlights Step 1. METHOD EDIT SCREEN You can use the cursor arrow keys, or the key to move from field to field.
  • Page 89 Editing the Current Method Using Method Mode In the RSP/PW field, press the corresponding softkey to enter the value for the “Smoothing” mode. This field displays either RSP or PW depending on the detec- tor’s configuration. For more information on selecting the correct value, see the “Detector Response Time”...

  • Page 90: Editing Timed Events

    Using Method Mode Editing the Current Method Editing Timed Events Timed events control the relay contact closures which in turn control auxiliary instruments (fraction collector, autosampler, etc.). You can actuate two timed events (TE1 and TE2) a maximum of twenty times while running the method. To edit the timed events, follow the procedure below.
  • Page 91 Editing the Current Method Using Method Mode TIMED EVENTS SCREEN To move to the TE1 data field, press the key. The timed event may be either O, enter C, or M. These letters indicate the following: O: Open - The relay opens at the time of the event. C: Close - The relay closes at the time of the event.

  • Page 92: Creating A New Method

    Using Method Mode Creating a New Method Creating a New Method The current method is the method that appears on the display. You can edit the current method by changing the method’s operating parameters. You can also create a new method. When you create a new method, the detector displays a one step method with the default operating parameters.

  • Page 93: Storing A Method

    Storing a Method Using Method Mode Storing a Method Once you make changes to the method displayed on the screen you can permanently save, or store the method. Any changes you make to a method are not saved until you store the method.
  • Page 94 Using Method Mode Storing a Method METHOD EDIT SCREEN Press F6 from within the Method Edit Screen. The Save Method Screen appears STOR prompting you to save changes to the method or to save the method with a new method name and number.

  • Page 95: Recalling A Method From The Directory

    Recalling a Method from the Directory Using Method Mode Recalling a Method from the Directory The Directory lists the stored methods and shows you the method number, status, name and date and time the method was last modified. Method Number Method Status Method Name Date and Time...
  • Page 96 Using Method Mode Recalling a Method from the Directory MAIN SCREEN Press F6 from the Main Screen to go to the Directory Screen. DIRECTORY SCREEN Using the cursor arrow keys, move down through the list of stored methods until the caret (>) is next to the method you want to recall, in this example, 2.

  • Page 97: Editing A Stored Method

    Editing a Stored Method Using Method Mode Editing a Stored Method You cannot edit the current method from the detector Directory. You can use the Directory to recall a stored method, or to edit a copy of the current method and save it as a new method.
  • Page 98 Using Method Mode Editing a Stored Method MAIN METHOD SCREEN To edit a stored method, press F6 from the Main Screen. The Directory Screen, similar to the one below, appears. DIRECTORY SCREEN caret Message line Scroll the list until the method you wish to edit is next to the caret (>). Press F1 and the following Edit Screen appears.

  • Page 99: Running A Method

    Running a Method Using Method Mode Running a Method You can run the current method by pressing the key on the keypad. To restart a stopped method you can press the key. This reinitializes the method to Step 1. reset While you are running a method, the options for editing that method are limited.

  • Page 100: Viewing The Chromatogram

    Using Method Mode Viewing the Chromatogram Viewing the Chromatogram Press the View key to display the detector baseline prior to a run, or to display the chromato- gram after the detector has been placed into run. The default scale for the Y axis is 1.0 AUFS.

  • Page 101: Using Sequences

    Using Sequences This chapter describes how to use Sequences on your Series 200 UV/VIS LC Detector. It includes the following information: • About Sequences • Defining a Sequence • Running a Method in Sequence • Stopping a Sequence...

  • Page 102: About Sequences

    Using Sequences About Sequences About Sequences A sequence is a series of stored methods that are linked together. A sequence allows you to automatically run a series of methods while the detector is unattended. The following are the possible states for a sequence: •...
  • Page 103 About Sequences Using Sequences The following table describes the parts of the Sequence Screen. Part of the Number Description Screen Indicates a line in the sequence. There can be from one to 20 lines in the sequence. METH The method number for the Set. The method must have one or more steps.

  • Page 104: Defining A Sequence

    Using Sequences Defining a Sequence Defining a Sequence Before you begin, you need to know the rules for defining a sequence and how to identify the methods that comprise the sequence. Rules for Defining a Sequence • If you have not already done so, you need to create and store in the Detector’s Directory all of the methods that you intend to include in the sequence.

  • Page 105: Identifying The Methods To Include In A Sequence

    Defining a Sequence Using Sequences Identifying the Methods to Include in a Sequence Before defining the sequence, you need to view the methods stored in the detector’s Directory so that you can identify the numbers and names of the methods you want to include in the sequence.

  • Page 106: Procedure For Defining A Sequence

    Using Sequences Defining a Sequence Procedure for Defining a Sequence METHOD SCREEN Press F5 from the Main Method Screen to go to the Sequence Screen. The Method Number for SET 1 is highlighted. A Set indicates the method number and the number of injections.

  • Page 107: Procedure For Defining A Sequence (Continued)

    Defining a Sequence Using Sequences Procedure for Defining a Sequence (continued) When you press the key or the key to add another set, the system uses enter insert the same method number and number of injections as in the previous set. Use the numeric keys to type in a new method number for the new set and number of injections.

  • Page 108: Setting Up A Sequence

    Using Sequences Setting up a Sequence Setting up a Sequence You can use the LINK command to set up the sequence. The LINK command makes the selected sequence the current, active sequence. Only one linked sequence is allowed. A sequence many contain one or more Sets. A Set is the combination of a method number and the number of injections.

  • Page 109: Running A Method In Sequence

    Running a Method in Sequence Using Sequences Running a Method in Sequence Once linked, when you press the Run key, the detector runs the first method in the sequence. When the first method has been run for the first injection, the detector becomes ready for the next injection or the next method in the sequence.

  • Page 110: Stopping A Sequence

    Using Sequences Stopping a Sequence Stopping a Sequence While a sequence is running, if needed, you can stop it. Once a sequence is linked, the follow- ing softkeys appear on the Sequence Screen: Softkey Description Allows you to select a SET number and review the parameters Allows you to break a running sequence at any time.

  • Page 111: Configuring The Detector

    Configuring the Detector This chapter describes how to configure your Series 200 UV/VIS LC Detector. It includes the following information: • Setting Configuration Options Setting up the Instrument Diagnostic Tests for Service Use Validating the Wavelength Calibration Checking the Instrument Log...

  • Page 112: Setting Configuration Options

    Configuring the Detector Setting Configuration Options Setting Configuration Options Setting the Detector configuration options involves the following tasks, which appear on the Configure menu: • Setting up the Instrument • Running Diagnostic Tests • Checking the Wavelength • Checking the Instrument Log 6 - 2...

  • Page 113: Setting Up The Instrument

    Setting Configuration Options Configuring the Detector Setting Up the Instrument This section describes how to set up the Detector and includes: • Setting up the Lamp • Setting the Screen Contrast and Screen Saver • Setting the Analog Output • Setting the System Clock •...
  • Page 114 Configuring the Detector Setting Configuration Options MAIN SCREEN From the Main Screen, press F7 to move to the Configure Screen. CNFG CONFIGURE SCREEN Press F1 to move to the Instrument Setup Screen. INST INSTRUMENT SETUP SCREEN 6 - 4...
  • Page 115 Setting Configuration Options Configuring the Detector Setting Up the Lamp You can use the Lamp Set-Up screen to set the wavelength range you want the lamp to cover. You make the change in this screen if you do not want to accept the default value for the installed lamp.
  • Page 116 Configuring the Detector Setting Configuration Options Setting the Screen Contrast The Screen Contrast Screen allows you to adjust the screen’s brightness. When you press the SCRN softkey, the Detector displays the Screen Contrast Screen. The contrast range is from 1 to 22, with 22 being the most bright. From the Instrument Setup Screen, press F2 to move to the Screen Contrast Screen.
  • Page 117 Setting Configuration Options Configuring the Detector Setting the Analog Output You can set the detector’s analog output options to correspond to the attached data handling system. When you press the ANLG softkey, the detector displays the Analog Output Setup Screen. This screen allows you to set the following: •...
  • Page 118 Configuring the Detector Setting Configuration Options Adjusting the Analog Offset To overcome any possible negative drift, you can make a correction by adjusting the analog offset value. The detector measures signals within a limited range. The range cannot go below zero or above one. If the signal drifts below the range, it cannot be seen on the recorder.
  • Page 119 Setting Configuration Options Configuring the Detector Adjusting the Computer Output The computer output value sets the number of volts per absorbance unit. You need to match the output from the detector to your data handling system. Refer to the operating manual for your data handling system to identify the required voltage.
  • Page 120 Configuring the Detector Setting Configuration Options Setting the Recorder Adjust Value The recorder adjust option allows you to calibrate the recorder. The following are the avail- able recorder adjust settings: • NORMAL: Sets the recorder to the normal setting. To test the recorder, select one of these settings: •...
  • Page 121 Setting Configuration Options Configuring the Detector Setting the System Clock If the default time or date set on the system clock is not correct for your time zone, you can use the System Clock Screen to change the date or time. The system clock is also used to time stamp errors that appear in the error log.
  • Page 122 Configuring the Detector Setting Configuration Options Adjusting the “Smoothing” Mode The “Smoothing” Mode options allow you to reduce noise coming from the detector. There are two available options, RSP and PW. The two options provide different ways to set the parameters;...
  • Page 123 Setting Configuration Options Configuring the Detector Setting Up the Leak Detector The Leak Detector Set-up Screen lets you determine the action the detector should take when an internal or external leak is detected. Each field on the Leak Detector Set-up Screen can have one of three states.
  • Page 124 Configuring the Detector Setting Configuration Options NOTE: for the messages shown on this page, “ON INTERNAL LEAK DETECTOR ERROR” and “ON EXTERNAL LEAK DETECTOR ERROR” ignore (IGNR) the messages. Notice that in this condition, the default condition is “STOP”. From the Instrument Setup Screen, press F8 to move to page two of the Instrument MORE Setup Screen.

  • Page 125: Diagnostic Tests For Service Use

    Screen. The Diagnostics Screen displays options for Operational and Hardware diagnostics. The tests assess the electronic and mechanical performance of the Detector. This section describes only how to access these options. For more information on detector diagnostics, contact your PerkinElmer service representative. MAIN SCREEN From the Main Screen, press F7 to move to the Configure Screen.

  • Page 126: Checking The Wavelength Calibration

    Configuring the Detector Setting Configuration Options Checking the Wavelength Calibration This section describes how to run the wavelength calibration check. You can run the wave- length calibration check when you suspect that you are getting incorrect results from the λ detector.
  • Page 127 Screen. If the wavelength calibration consistently produces incorrect values, contact your PerkinElmer service representative. The detector can only be recalibrated by a PerkinElmer engineer. Your service representative can perform monthly checks on the wavelength calibration if your lab requires it.

  • Page 128: Checking The Instrument Log

    The system has detected an error in one of the sensors used to determine the position of the monochrometer. This error will render the detector unusable. It cannot be customer corrected and you should contact your local PerkinElmer service representative. • A/Z OUT OF RANGE The system autozeros the output signal by attempting to balance the reference and sam- ple signals.
  • Page 129 A Short or Open error on the Internal Leak Detector implies an error in the leak detector electronics and you should contact your local PerkinElmer service representative. A Short or Open error on the External Leak Detector may be caused by incorrect installation of the leak detector.
  • Page 130 Configuring the Detector Setting Configuration Options Press F5 to display the LOG Screen. The LOG Screen displays the Detector’s software version number, the lamp hours, and display hours. Press the down arrow key to move from field to field. You can modify the lamp hours if necessary, for example, you can reset the lamp hours when you install a new lamp in the Detector.

  • Page 131: Maintenance

    Maintenance This chapter describes routine and periodic maintenance procedures that must be performed to keep the detector in optimal working condition. The chapter also provides systematic pro- cedures for isolating and repairing problems with the instrument should they occur.

  • Page 132: General Performance

    Maintenance General Performance General Performance This chapter contains the information needed to keep the Series 200 UV/VIS LC Detector in optimal operating condition. With proper care and adherence to good chromatographic prin- ciples, the Series 200 UV/VIS LC Detector will give you the high sensitivity and operating versatility it was designed to provide.

  • Page 133: Maintenance Guidelines

    PerkinElmer replacement part numbers are referenced throughout this section; however, when ordering replacements, refer to the HPLC Supplies Catalog or to a PerkinElmer Prod- uct Description List. To save time and money, refer to the Troubleshooting Guide in this chapter before perform- ing any service.

  • Page 134: Routine Maintenance

    Maintenance Routine Maintenance Routine Maintenance Flowcell Maintenance Flowcell Flushing The detector flowcell should be flushed thoroughly before and after each chromatographic run with a volume of mobile phase equal to 10 times the volume of the installed flowcell. If buffers are being used, the entire system should he flushed with HPLC grade water. Other- wise, the flowcell may retain residues which could cause excessive noise or drift.
  • Page 135 Routine Maintenance Maintenance Inlet fitting Outlet fitting Heat exchanger Flowcell screw Flowcell door Figure 7-1. Flowcell location (Top/front view of Detector) Heat exchanger screws Flowcell Reference cell Figure 7-2. Flowcell compartment (Front view of Detector) 7 - 5...
  • Page 136 Maintenance Routine Maintenance Disassembling and Cleaning the Flowcell If the flowcell shows signs of leakage around the flowcell apertures, or if the flowcell win- dows are cracked or too dirty to clean by flushing, then the cells should be disassembled and rebuilt.
  • Page 137 Routine Maintenance Maintenance Reassembling the Flowcell Please do not touch the quartz windows with fin- gers. Skin oils will absorb UV and cause noise. 1. Place a window in the seat in the rear of the flowcell body. Place the entrance aperture (the one with threaded screw holes and the smaller center hole) onto the flowcell body and window so the screw holes lineup with those in the flowcell body.

  • Page 138: Photodiode And Monochromator Windows

    Maintenance Routine Maintenance Photodiode and Monochromator Windows Over time, the photodiode windows in the detector head (which cover and protect the sample and reference photodiodes) and the monochromator exit windows (which prevent the entry of dust into the monochromator) may develop an invisible dirt layer which may be highly absorbent in the low UV.
  • Page 139 Routine Maintenance Maintenance Cleaning the Monochromator Exit Windows 1. Open the detector head. 2. Remove the reference (dry cell) and sample cells. 3. The exit windows are mounted in an assembly which is screwed into the flowcell plate. 4. Remove the window housings by inserting a large blade screwdriver into the slots in the window housings and turning carefully counter clockwise.
  • Page 140 Maintenance Routine Maintenance Inserting the Monochromator Exit Windows 1. Replace the windows and retaining rings into the housing assembly. This assembly must be reassembled properly or instrument performance will be impaired. 2. Place the completed window housing assembly into the flowcell clamp and retighten into the flowcell plate.

  • Page 141: Lamp Replacement

    Routine Maintenance Maintenance Lamp Replacement An aged lamp is the most common cause of excessive baseline noise or drift, especially at low UV wavelengths. Lamp life may be read on the CNFG LOG screen. The detector lightsource is the only Series 200 UV/VIS LC Detector component that may require routine replace- ment.
  • Page 142 Maintenance Routine Maintenance WARNING Hot Replace lamp with PerkinElmer part number N292-0149. Avertissement LA LAMPE EST TRÈS CHAUDE!! Remplacer la lampe par une lampe PerkinElmer, numéro de pièce N292-0149. 5. Unscrew the knurled screw that is holding the front panel in place.
  • Page 143 Routine Maintenance Maintenance Removing the Lamp (Starting at the Rear Panel) 1. Turn the detector power switch to the OFF position and unplug the AC line cord from the AC outlet. 2. Allow the detector to cool for 15-30 minutes before proceeding. 3.
  • Page 144 Check the instrument’s baseline for excessive noise or drift. Excessive noise and drift after a lamp change may indicate that the lamp is improperly seated. Remove the lamp, reinstall, and recheck. If any problems persist, call your PerkinElmer service engineer for further assistance.

  • Page 145: Troubleshooting Detector Problems

    50 psi backpressure device to the exit side of the flowcell (PerkinElmer PN 0990-7126). This causes the bubble to redissolve and flush from the flowcell. There is lit- tle danger of damage if backpressure is applied since PerkinElmer flowcells are rated to 500 psi.
  • Page 146 Maintenance Troubleshooting Detector Problems Mobile Phase Degassing Degassing refers to the removal of dissolved gas from a liquid by mechanical means. Three methods are commonly used. • Vacuum degassing • Sonication • Helium Degassing HPLC mobile phases should be degassed after mixing! The mechanical action of thoroughly mixing mobile phase components may reintro- duce atmospheric gases back into solution.

  • Page 147: Column Contaminants

    Troubleshooting Detector Problems Maintenance Column Contaminants Due to the detector’s high sensitivity, the HPLC column must be optimally maintained. In some instances, contaminants may wash off the column and affect the flowcell. This condi- tion often appears as a slow but steady upward or downward shift in the detector baseline over the course of a run (especially during gradient elution).

  • Page 148: Troubleshooting Methods

    Maintenance Troubleshooting Methods Troubleshooting Methods Users should consult this section if a problem develops or an instrument function fails to perform as expected. The procedure outlined below is a step-by-step approach to trouble- shooting the Series 200 UV/VIS LC Detector. Unlike the performance tests, which concen- trate on the detector’s output, these tests concern themselves with specific internal detector functions.
  • Page 149 Monochromator internal optics are not field replaceable. Realignment following repair or replacement can only be done in an PerkinElmer service facility. The detector performance required by the user determines the useful life of a lamp. Users who run their detectors at the highest sensitivities (lamp intensities) may need to replace their lamps more frequently than less demanding users.
  • Page 150 Maintenance Troubleshooting Methods 10-12 minutes for the monochromator to be completely filled. Continue this nitrogen purge while the instrument is in use. Purging is particularly important when an instru- ment is removed from a cold room to a high humidity room at ambient temperature. Compressed gas cylinders must be safely attached to the wall or bench by means of approved brackets or chains.

  • Page 151: Troubleshooting Guide

    Troubleshooting Methods Maintenance Troubleshooting Guide Symptom Probable Cause Possible Remedy No power to detector Not plugged into an AC outlet Plug the detector line cord into an AC outlet Blown fuse Replace the fuse. Faulty power supply Repair or replace the power supply.
  • Page 152 Maintenance Troubleshooting Methods Symptom Probable Cause Possible Remedy Detector response is Loose lamp wires Check lamp wires NOT steady at any set- ting Faulty lamp Replace the lamp and align the new lamp Excessive line voltage Check your line voltage output fluctuation Inappropriate solvent Flush the entire system with...
  • Page 153 Troubleshooting Methods Maintenance Symptom Probable Cause Possible Remedy Successive results Deteriorated chemical sample Visually check for change. make with the same sample fresh sample, clean flowcell, are not reproducible and re-analyze. Faulty injector valve Replace rotor seal. Change in mobile phase Prepare new mobile phase.
  • Page 154 Maintenance Troubleshooting Methods Symptom Probable Cause Possible Remedy Abnormal noise (cont.) Check whether the detector Flush the flowcell with clean electronics are cause (elimi- solvent and purge with dry nate solvent as cause) nitrogen. Run a baseline to check for noise. Replace the flowcell with the “Dummy”...

  • Page 155: Service

    PerkinElmer immediately. Instrument service may be performed either in the field or at PerkinElmer. For out of warranty instruments, returning the unit to PerkinElmer will normally be less expensive than field service since no travel time will be charged. Warranted instruments are covered by PerkinElmer for labor, travel and service.
  • Page 156 Maintenance Troubleshooting Methods 7 - 26...

  • Page 157: Principles Of

    Principles of Operation This chapter describes the principles of operation of the Series 200 UV/VIS LC Detector and also provides some guidelines on determining appropriate values for key parameters. Topics include: • Theory of Operation • Application Notes...

  • Page 158: Theory Of Operation

    Principles of Operation Theory of Operation Theory of Operation Beer’s Law The Series 200 UV/VIS LC Detector belongs to a class of analytical instruments known as spectrophotometers. This class of optical instruments measures the transmittance of light through a cell containing a solution of chemical compounds. The light which has passed through the cell is collected and converted to an electrical current by a silicon photodiode.
  • Page 159 Theory of Operation Principles of Operation Figure 8-1. Diagram showing Beer’s Law. Beer’s Law can be expressed in mathematical form as follows: Absorbance = log (I /I) = abc = Intensity of light beam at entrance of flowcell I = Intensity of light at exit of flowcell a = Molar absorptivity b = Cell pathlength c = Solute concentration...

  • Page 160: Optical Design

    Principles of Operation Theory of Operation Optical Design In order to make an absorbance determination, two measurements must be made, I and I. The ratio I/I defines transmittance. In a single beam absorption detector, the measurement of I is made with no sample in the light beam, and I is measured after the sample is placed in the beam.
  • Page 161 Series 200 UV/VIS LC Detector uses a bandwidth of 5 nm. In the PerkinElmer dual-beam design, the intensities of the light passing through the sam- ple flowcell and the reference cell are monitored. After being converted by the photodiodes to...

  • Page 162: Design Considerations

    Principles of Operation Design Considerations Design Considerations The Series 200 UV/VIS LC Detector is based on a modular design with each module dedi- cated to an internal function. This approach makes testing and servicing convenient and easy. If difficulty arises with a particular component, in most cases the module or sub-mod- ule containing that component may be field replaced to minimize downtime.

  • Page 163: Application Notes

    Application Notes Principles of Operation Application Notes Wavelength Selection The proper selection of a wavelength for a particular analysis is paramount in the operation of any absorbance detector. The choice of wavelength(s) can influence both the sensitivity and selectivity of a method. The first step in determining the optimal wavelength for a chromatographic method is to obtain a UV absorbance spectrum for the compound(s) of interest.

  • Page 164: Detector Response Time

    A two pole filter consists of a pair of resistors and a pair of capacitors along with an active device such as an operational amplifier. Two pole filters provide a larger S/N ratio than single pole filters, and therefore PerkinElmer currently uses two pole filters in their detectors (see Figure 8-3).
  • Page 165 Application Notes Principles of Operation There are various forms of noise. Electronic noise can arise from line voltage fluctuations, the detector's circuitry, and radio frequency interference. Short term noise has frequencies that range from 1 cycle/1 sec to 1 cycle/5 min. The very wide peaks and valleys observed in long term noise can arise from system variations such as flow rate and pressure variations, teaks, temperature variations, aging lamps, and gas in the detector cell.
  • Page 166 Principles of Operation Application Notes Rise time, on the other hand, is a non-mathematical description that is used for both single and double pole filters. If the input signal is suddenly changed (a step change), the detector requires a short period of time to register this change in its output signal. The rise time is defined as the time it takes the output signal to rise from 10% of its final value to 90% of its final value (see Figure 8-4).

  • Page 167: Enabling

    This chapter describes how to configure your Series 200 UV/VIS LC Detector to allow exter- nal control by Turbochrom. You can set the detector to allow external control by Turbo- chrom, version 6.1.2 or higher. Turbochrom is a PerkinElmer data handling system. This chapter includes the following information: •...

  • Page 168: Configuring Turbochrom With The Series 200 Uv/Vis Detector

    Enabling External Control Configuring Turbochrom with the Series 200 UV/VIS Detector Configuring Turbochrom with the Series 200 UV/VIS Detector The Series 200 UV/VIS Detector can be controlled from Turbochrom and may be configured in one of two modes: • As a 785 detector. •...
  • Page 169 Configuring Turbochrom with the Series 200 UV/VIS Detector Enabling External Control IMPORTANT In the “Series 200 UV/VIS mode,” the transmitted digital absorbance values must always be positive. To allow for negative drift in the chromatogram, a 0.3AU offset is automatically applied to the digital chromatographic signal.
  • Page 170 Enabling External Control Configuring Turbochrom with the Series 200 UV/VIS Detector 9 - 4...

  • Page 171: Index

    Index Numerics Scanning 4-20 Viewing 2-15 Cleaning Monochromator Exit Windows Photodiode windows Column 2-19 scavanger AC Line Cord 2-10 COMP and REC outputs Installing Computer Output AC Line Cord Selections Adjusting AC line voltage 2-10 Computer/Integrator Connections Adjusting Analog Offset Configuration Options Adjusting Computer Output Setting...
  • Page 172 Index Front Panel view General performance 1-14 S-20 F1 through F8 options Moving S-10, 8-6 Flowcell Powering up Cleaning Rear Panel view Disassembling Response time Flushing Setting Up 1-20 Installing Specifications Maintenance Unpacking S-11 Reassembling Weight Removing Detector Control Parameters 6-16 Replacing Editing...
  • Page 173 Index S-13 Instrument Performance Verification Setting Operating Parameters in Instrument Setup Manual mode 6-13 Leak Detector Switching to Instrument Setup Screen Using Internal Warning Labels Manual Operation S-13 Manual Settings Screen, Parts of Method 4-12 Creating Definition Editing the current Keyboard 4-15 Recalling...
  • Page 174 Index Overview of the instrument Safety Electrical S-20 High voltage 4-7, 6-12 Peak Width S-20 Moving the detector Photodiode S-13 Suitability test Photodiode window 2-18 Sample In Connector Photodiode windows 2-18 Sample Out Connector Cleaning 7-15 Saturation, gas 2-18 Plumbing connections, Functions of Scanning 2-15 Plumbing Connections, Making...
  • Page 175 Index S-18 Auto-Ignition Temperature S-16 Low boiling points 7-16 7-16 Vacuum degassing Sonication Viewing Specifications 6-18 1-20 Error Log Instrument 4-20 Viewing the Chromatogram Start-up Kit 5-10 Voltage selector card Stopping a sequence S-11 Selecting the voltage Storage Conditions Voltage selector card, location 4-13 STORD status 4-17...
  • Page 176 Index In-6...
  • Page 177 To place an order for supplies and many replacement parts, request a free e-ssentials catalog or ask for information, visit our websiteat www.perkinelmer.com The most up-to-date information on part numbers, product brochures, systems modules, spare parts, and application notes are in the PerkinElmer website. Telephone: •...

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