PerkinElmer 200 UV/VIS Series User Manual page 154

Maintenance
Symptom
Abnormal noise (cont.)
High flowcell back-
pressure
Up scale drift (higher
absorbance levels)
7 - 24
Probable Cause
Check whether the detector
electronics are cause (elimi-
nate solvent as cause)
Incorrect lamp alignment
Clouded flowcell
Lamp near the end of its life
span
Dirty flowcell
Plugged flowcell
Chromatographic problems
Flowcell leak
Clouded flowcell window
Dirty column
Unstable environment
Unstable mobile phase
Incorrect lamp alignment
Lamp tailing
Troubleshooting Methods
Possible Remedy
Flush the flowcell with clean
solvent and purge with dry
nitrogen. Run a baseline to
check for noise.
Replace the flowcell with the
"Dummy" cell.
Check lamp installation
Clean or replace flowcell.
Replace the lamp
Clean and/or rebuild the
flowcell
Clean or rebuild the flowcell
Replace the flowcell with the
"Dummy" cell and recheck.
Repair or replace the flowcell
Replace the flowcell with the
"Dummy" cell.
Clean or rebuild the flowcell
Flush/regenerate the column
Replace the column and equili-
brate a new column
Maintain a constant tempera-
ture, humidity, etc.
Replace with stable mobile
phase
Check lamp installation
Replace lamp