PerkinElmer 200 UV/VIS Series User Manual page 152

Maintenance
Symptom
Detector response is
NOT steady at any set-
ting
7 - 22
Probable Cause
Loose lamp wires
Faulty lamp
Excessive line voltage
fluctuation
Inappropriate solvent
Air bubbles in the solvent
inlet line
Air bubbles in the flowcell
Unstable sample
Dirty column
Flowcell leaking
Leak in the flowcell area
Troubleshooting Methods
Possible Remedy
Check lamp wires
Replace the lamp and align the
new lamp
Check your line voltage output
Flush the entire system with
new solvent
Degas the solvent
Check that solvent level is not
too low
Apply back pressure at outlet or
connect a backpressure device
(P/N 0990-7126)
Degas the mobile phase.
Connect a backpressure valve
(P/N 0990-7126)
Bypass the column and pump
mobile phase at 5 mL/min (not
through the column) for 30 sec-
onds to dislodge air
Make a fresh sample, clean the
flowcell, then re-analyze
Flush/regenerate column
Check the flowcell and repair or
replace if necessary
Tighten the column outlet fit-
ting if necessary
Tighten the detector waste line
for crimping and replace if nec-
essary