PerkinElmer 200 UV/VIS Series User Manual page 153

Troubleshooting Methods
Symptom
Successive results
with the same sample
are not reproducible
Peaks are broad
(loss of resolution)
Abnormal noise
Probable Cause
Deteriorated chemical sample
Faulty injector valve
Change in mobile phase
Column losing efficiency
(peak broadening or
retention time changing)
Column losing efficiency
Change in mobile phase
Chromatographic or electric
problems
Column aging
Bubbles in the flowcell
Plumbing leaks
Possible Remedy
Visually check for change. make
fresh sample, clean flowcell,
and re-analyze.
Replace rotor seal.
Prepare new mobile phase.
Clean flowcell. Pump new
mobile phase until stable base-
line is achieved. Re-inject
sample.
Invert and flush the column.
Replace and equilibrate with
new column.
Invert and flush the column.
If regeneration is unsuccessful,
replace and equilibrate with
column.
Prepare a new mobile phase.
Clean flowcell. Pump the new
mobile phase until stable base-
line is achieved. Re-inject
sample.
Install the "Dummy" cell
Install a power line filter.
If abnormal noise stops, check
for chromatographic problems.
If abnormal noise continues,
check the lamp or other
electrical diagnostics.
Install a new column
Flush out the bubbles
Check all tubing connections for
tightness
Check the flowcell and column
outlet connection
Maintenance
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