Operating Principle; Sampling Sequence - Agilent Technologies InfinityLab LC Series User Manual

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Introduction

Operating Principle

Operating Principle

Sampling Sequence

The Vialsampler processor continuously monitors the movements of the
vialsampler components during the sampling sequence. The processor defines
specific time windows and mechanical ranges for each movement. If a specific
step of the sampling sequence can't be completed successfully, an error
message is generated.
During the sampling sequence, the solvent bypasses the vialsampler via the
injection valve. The gripper arm selects the sample vial, either from a static
sample rack, or from external vial positions. The gripper arm places the
sample vial below the injection needle. The required volume of sample is
drawn into the sample loop by the metering device. Sample is applied to the
column when the injection valve returns to the mainpass (main path) position
at the end of the sampling sequence.
The sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The piston of the metering device moves to the initialization position.
3 The gripper arm moves from the home position, and selects the vial. At the
4 The gripper moves into the needle station and stops in the draw position.
5 The needle lowers into the vial.
6 The metering device draws the defined sample volume.
7 The needle lifts out of the vial.
8 The gripper arm moves out slightly and stops in the wash position
9 The needle moves downwards and dips into the wash well of the wash port.
10 The needle moves back.
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same time, the needle lifts out of the seat.
Simultaneously the peristaltic pump delivers the flush solvent.
only if automated needle wash is selected. If this feature is disabled, the gripper arm positions the
sample vial directly below the needle (Step 4) and lowers the needle into the vial.
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Agilent InfinityLab LC Series Vialsamplers User Manual
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