Using An Injector Program - Agilent Technologies InfinityLab LC Series User Manual

6
Optimizing Performance
Optimization for Lowest Carry-over

Using an Injector Program

The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is
performed at the end of the equilibration time to ensure that the bypass grove
is filled with the start concentration of the mobile phase. Otherwise the
separation could be influenced, especially if microbore columns are used.
Example
Outside wash of needle in vial p2-A1 before injection
Injector program:
• Draw x.x (y) μl from sample
• WASH vial location p2-A1
• Inject
Overlapped injection together with additional injection valve switching is not possible.
N O T E
134
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass (main path)
Valve bypass
Valve mainpass (main path)
Agilent InfinityLab LC Series Vialsamplers User Manual