General Recommendations For Fast Injection Cycle Times - Agilent Technologies InfinityLab LC Series User Manual

6
Optimizing Performance
Fast Injection Cycle and Low Delay Volume
For the last injection of the sequence with overlapped injections it has to be considered
N O T E
that for this run the injection valve is not switched as for the previous runs and
consequently the injector delay volume is not bypassed. This means the retention times
are prolonged for the last run. Especially at low flow rates this can lead to retention time
changes which are too big for the actual calibration table. To overcome this it is
recommended to add an additional "blank" injection as last injection to the sequence.

General Recommendations for Fast Injection Cycle Times

As described in this section, the first step to provide short cycle times are
optimizing the chromatographic conditions. If this is done the autosampler
parameter should be set to:
• Overlapped injection mode
• Increase of draw and eject speed for large injection volumes
• Add at last run a blank, if overlapped injection is used
To reduce the injection time, the detector balance has to be set to OFF.
Agilent InfinityLab LC Series Vialsamplers User Manual 137