Agilent Technologies InfinityLab LC Series User Manual
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Agilent Technologies InfinityLab LC Series User Manual

Variable wavelength detectors
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Agilent InfinityLab LC Series VWD User Manual

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Summary of Contents for Agilent Technologies InfinityLab LC Series

  • Page 1 Agilent InfinityLab LC Series Variable Wavelength Detectors User Manual Agilent InfinityLab LC Series VWD User Manual...
  • Page 2 12.211 (Technical Data) and 12.212 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation). Agilent InfinityLab LC Series VWD User Manual...
  • Page 3 This chapter provides information on how to set up the detector for an analysis and explains the basic settings. Optimizing the Detector This chapter provides information on how to optimize the detector. Troubleshooting and Diagnostics Overview about the troubleshooting and diagnostic features. Agilent InfinityLab LC Series VWD User Manual...
  • Page 4 This chapter describes the detector in more detail on hardware and electronics. LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. Appendix This chapter provides addition information on safety, legal and web. Agilent InfinityLab LC Series VWD User Manual...

  • Page 5: Table Of Contents

    Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings Scanning with the VWD Agilent Local Control Modules Preparing the Module Leak and Waste Handling Setting up an Analysis Solvent Information Agilent InfinityLab LC Series VWD User Manual...
  • Page 6 Detector Error Messages Test Functions Introduction Intensity Test Cell Test Wavelength Verification-Calibration ASTM Drift and Noise Test Quick Noise Test Dark Current Test Holmium Oxide Test D/A Converter (DAC) Test Other Lab Advisor Functions Agilent InfinityLab LC Series VWD User Manual...
  • Page 7 Micro Flow Cell 3 mm / 2 µl Semi-micro Flow Cell (Parts) High Pressure Flow Cell (Parts) Cuvette Holder (Parts) Accessory Kit Identifying Cables Cable Overview Analog Cables Remote Cables CAN Cable RS-232 Cables USB Cables Agilent InfinityLab LC Series VWD User Manual...
  • Page 8 Dynamic Host Configuration Protocol (DHCP) Manual Configuration Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet Agilent InfinityLab LC Series VWD User Manual...

  • Page 9: Introduction To The Variable Wavelength Detector

    Photo Diodes Assemblies Photo Diode ADC (analog-to-digital converter) Dual-Wavelength Mode Leak and Waste Handling Leak Sensor Waste Concept Operating Principle This chapter gives an introduction to the detector, instrument overview and internal connectors. Agilent InfinityLab LC Series VWD User Manual...

  • Page 10: Introduction To The Detector

    • built-in electronic temperature control (ETC) for improved baseline stability, • built-in holmium oxide filter for fast wavelength accuracy verification. Agilent InfinityLab LC Series VWD User Manual...

  • Page 11: Product Description

    High productivity can be achieved with fast analysis at up to 120 Hz data rates. Status indicator Flow cell Lamp housing window and air inlet Power switch Leak drain Figure 1 Overview of the detector Agilent InfinityLab LC Series VWD User Manual...

  • Page 12: Product Description G7114B

    High productivity can be achieved with fast analysis at up to 240 Hz data rates. Status indicator Flow cell Lamp housing window and air inlet Power switch Leak drain Figure 2 Overview of the detector Agilent InfinityLab LC Series VWD User Manual...

  • Page 13: Optical System Overview

    370 nm to reduce higher order light. Deuterium lamp Filter assembly Entrance slit Lens Mirror M1 Sample diode Grating Flow cell Mirror M2 Beam splitter Reference diode Figure 3 Optical Path of the Variable Wavelength Detector Agilent InfinityLab LC Series VWD User Manual...

  • Page 14: Flow Cell

    Outlet i.d. 0.30 0.17 0.17 0.17 Outlet length Total volume 60.77 14.49 14.00 60.77 µL Materials in contact SST, quartz, PTFE, SST, quartz, PTFE SST, quartz, PTFE SST, quartz, Kapton with solvent PEEK Agilent InfinityLab LC Series VWD User Manual...

  • Page 15: Lamp

    Entrance Slit Assembly The entrance slit assembly has an exchangeable slit. The standard one has a 1-mm slit. For replacement and calibration purposes to optimize the alignment, a slit with a hole is needed. Agilent InfinityLab LC Series VWD User Manual...

  • Page 16: Filter Assembly

    > 370 nm HOLMIUM holmium oxide filter for wavelength check SHUTTER for measurement of dark current of photo diodes A photo sensor determines the correct position. Agilent InfinityLab LC Series VWD User Manual...

  • Page 17: Mirror Assemblies M1 And M2

    The photo diode current is directly converted to digital data direct photo current digitalization. The data is transferred to the detector main board . The photo diode ADC boards are located close to the photo diodes. Agilent InfinityLab LC Series VWD User Manual...

  • Page 18: Dual-Wavelength Mode

    • Wavelength settings are timetable programmable (depends if enough time for implementation is available), • switching from Single-Wavelength mode to Dual-Wavelength mode is NOT timetable programmable, • filter settings are not timetable programmable. Agilent InfinityLab LC Series VWD User Manual...

  • Page 19: Leak And Waste Handling

    Introduction to the Variable Wavelength Detector Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.
  • Page 20 Introduction to the Variable Wavelength Detector Leak and Waste Handling Figure 6 Infinity II Leak Waste Concept (Flex Bench installation) Agilent InfinityLab LC Series VWD User Manual...
  • Page 21 Introduction to the Variable Wavelength Detector Leak and Waste Handling Figure 7 Infinity II Single Stack Leak Waste Concept (bench installation) Agilent InfinityLab LC Series VWD User Manual...
  • Page 22 Figure 8 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container. Agilent InfinityLab LC Series VWD User Manual...

  • Page 23: Leak Sensor

    1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series VWD User Manual...

  • Page 24: Operating Principle

    Introduction to the Variable Wavelength Detector Operating Principle Operating Principle Flow cell Waste Column Figure 9 Hydraulic path Agilent InfinityLab LC Series VWD User Manual...

  • Page 25: Site Requirements And Specifications

    Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Specifications Specification Conditions This chapter gives information on environmental requirements, physical and performance specifications. Agilent InfinityLab LC Series VWD User Manual...

  • Page 26: Site Requirements

    Make sure the power connector of the instrument can be easily reached  and unplugged. Provide sufficient space behind the power socket of the instrument to  unplug the cable. Agilent InfinityLab LC Series VWD User Manual...
  • Page 27 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
  • Page 28  If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. Agilent InfinityLab LC Series VWD User Manual...

  • Page 29: Physical Specifications

    Up to 4600 m (15092 ft) For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, EN, CSA, UL ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series VWD User Manual...

  • Page 30: Performance Specifications

    Wavelength accuracy ±1 nm, self-calibration with deuterium lines, verification with holmium oxide filter Wavelength precision <±0.1 nm Slit width 6.5 nm typical over whole wavelength range Time programmable Wavelength, polarity, peak width, lamp on/off Agilent InfinityLab LC Series VWD User Manual...
  • Page 31 Pilot and Agilent Lab Advisor software. Leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. Tracking of flow cells and lamps with RFID (radio fre- quency identification) tags Agilent InfinityLab LC Series VWD User Manual...
  • Page 32 Preparative:0.06 mm cell path length and 50 bar (725 psi) pressure maxi- High pressure:14 µL volume, 10 mm cell path length and 400 bar (5801 psi) pressure maximum Spectral tools Stop-flow wavelength scan Analog output Recorder/Integrator 100 mV or 1 V, 1 output Agilent InfinityLab LC Series VWD User Manual...
  • Page 33 Pilot and Agilent Lab Advisor software. Leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas. Tracking of flow cells and lamps with RFID (radio fre- quency identification) tags Agilent InfinityLab LC Series VWD User Manual...

  • Page 34: Specification Conditions

    Performance tests should be done with a completely warmed up optical unit (> one hour). ASTM measurements require that the detector should be turned on at least 24 hours before start of testing. Agilent InfinityLab LC Series VWD User Manual...

  • Page 35: Using The Module

    Set up the Detector with Agilent Open Lab ChemStation The Detector User Interface Detector Control Settings Method Parameter Settings Scanning with the VWD Agilent Local Control Modules This chapter explains the essential operational parameters of the module. Agilent InfinityLab LC Series VWD User Manual...

  • Page 36: Magnets

    Using the Module Magnets Magnets Magnets in doors of pumps, autosamplers, detectors, and fraction collectors. Agilent InfinityLab LC Series VWD User Manual...

  • Page 37: Turn On/Off

    Using the Module Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On Agilent InfinityLab LC Series VWD User Manual...
  • Page 38 Using the Module Turn on/off Turn instrument On/Off with the control software. Power switch: Off Agilent InfinityLab LC Series VWD User Manual...

  • Page 39: Status Indicators

    5. Resident mode (blinking) - for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent InfinityLab LC Series VWD User Manual...

  • Page 40: Instrument Configuration

    1 Set the switches of the Configuration switch at the rear of the module: a All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP. c Switch 5 UP and others DOWN: modules uses STORED address Agilent InfinityLab LC Series VWD User Manual...
  • Page 41 Using the Module Instrument Configuration 2 Enter the setup information (MAC / IP address and/or Instrument Name). a Agilent OpenLab ChemStation (Configure Instrument): b Lab Advisor (Instrument Overview - Add Instrument): Agilent InfinityLab LC Series VWD User Manual...

  • Page 42: Set Up The Detector With Agilent Open Lab Chemstation

    Figure 10 ChemStation Method and Run Control (just detector is shown) After successful load of the OpenLab ChemStation, you should see the module as an active item in the graphical user interface (GUI). Agilent InfinityLab LC Series VWD User Manual...

  • Page 43: The Detector User Interface

    • EMF warning. Maintenance required (red) Important: The EMF settings can be accessed via Agilent Lab Advisor. The limit(s) can be changed. Based on the limit, the User Interface displays the above status. Agilent InfinityLab LC Series VWD User Manual...
  • Page 44 Identify Module (Status LED will blink) • Perform a Balance • Switch the UV-lamp on/off (same as click on button “Make Device Ready/Turn device off (standby)”) • Take / Abort Scans (during flow off) Agilent InfinityLab LC Series VWD User Manual...

  • Page 45: Detector Control Settings

    If no RFID tag cell is used, detector icon will become gray (cell tag not ready) and analysis is disabled. • At Power On: automatic lamp-on at power on. • Automatic Turn On: automatic detector power on. Agilent InfinityLab LC Series VWD User Manual...

  • Page 46: Method Parameter Settings

    (does not show the Instrument Curves tab). Figure 11 Method parameter settings For additional help and support: Highlight the desired cell and press F1. A help NOTE screen will open with additional information and documentation about the topic. Agilent InfinityLab LC Series VWD User Manual...
  • Page 47 G7114A Peakwidth settings up to 120 Hz The G7114A VWD has a data rate of up to 120 Hz. The G7114B VWD has a data rate of up to 240 Hz. Figure 14 G7114B Dual Wavelength Settings Agilent InfinityLab LC Series VWD User Manual...
  • Page 48 “Warm up of the Detector” on page 75. Scan Range / Step: Stop-Flow scan range / step. Access to the scan feature is only possible during run. See “Scanning with the VWD” on page 50. Agilent InfinityLab LC Series VWD User Manual...
  • Page 49 These signals can be used in addition to the normal baseline signal to determine whether correlation to temperature or volt- age/current of the lamp. These signals are available via the Agilent ChemStation Online Plot/Data Signal and/or Agilent Lab Advisor Software. Agilent InfinityLab LC Series VWD User Manual...

  • Page 50: Scanning With The Vwd

    Turning off the pump would stop the run and no access to the sample scan is NOTE possible. Agilent InfinityLab LC Series VWD User Manual...
  • Page 51 Online Spectra Window 6 Select Sample Scan. A sample scan is taken in the range defined under Miscellaneous in Table 5 on page 47 and displays the result (Sample Scan minus Blank Scan). Agilent InfinityLab LC Series VWD User Manual...

  • Page 52: Agilent Local Control Modules

    • a clear overview of the system status • control functionalities • access to method parameters and sequences • a logbook showing events from the modules • diagnose tests Agilent InfinityLab LC Series VWD User Manual...
  • Page 53 Factory installed software – flat dialog structure, user configurable interface, enhanced sequence engine, for example with wait for baseline stabilization, diagnosis with passed/failed. • GLP – System logbook and module log-books record errors, unusual events and maintenance activities for GLP traceability. Agilent InfinityLab LC Series VWD User Manual...

  • Page 54: Preparing The Module

    Preparing the Detector Preparing the HPLC System Running the Sample and Verifying the Results Solvent Information This chapter provides information on how to set up the detector for an analysis and explains the basic settings. Agilent InfinityLab LC Series VWD User Manual...

  • Page 55: Leak And Waste Handling

    For details, see the usage guideline for the solvent cabinet. A printed copy of the NOTE guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. Agilent InfinityLab LC Series VWD User Manual...

  • Page 56: Waste Concept

    1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series VWD User Manual...

  • Page 57: Setting Up An Analysis

    (when available in the system) and the pump. Solvents containing volatile ingredients will slightly lose these. Therefore priming of the pumping system is required before starting an application. Agilent InfinityLab LC Series VWD User Manual...
  • Page 58 2 Flush all tubes with at least 30 mL of solvent. 3 Set flow to required value of your application and close the purge valve. Pump for approximately 10minutes before starting your application. NOTE Agilent InfinityLab LC Series VWD User Manual...

  • Page 59: Requirements And Conditions

    59: Table 9 Conditions Flow 1.5 mL/min Stoptime 8 min Solvent 100% (30% water/70% Acetonitrile) Temperature Ambient Wavelength sample 254 nm Injection Volume 1 µL Column Temperature (optional): 25 °C or ambient Agilent InfinityLab LC Series VWD User Manual...
  • Page 60 Variations in solvent quality, column packing, standard concentration and column temperature will all have a potential effect on peak retention and response. Figure 16 Typical Chromatogram with UV-detector Agilent InfinityLab LC Series VWD User Manual...

  • Page 61: Preparing The Detector

    Sampler (prepare the standard isocratic sample into a vial). e Solvents (fill water and Acetontrile into the solvent bottles). 5 Load the default method. 6 Pump the water/acetonitrile (30/70 %) mobile phase through the column for 10 min for equilibration. Agilent InfinityLab LC Series VWD User Manual...
  • Page 62 Preparing the Module Setting up an Analysis 7 Select the menu item Run Control> Sample Info and enter information about this application. Click OK to leave this screen. Figure 17 Sample Info Agilent InfinityLab LC Series VWD User Manual...

  • Page 63: Running The Sample And Verifying The Results

    2 This will start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram. Figure 18 Chromatogram with Isocratic Test Sample Information about using the Data Analysis functions can be obtained from the NOTE Using your ChemStation manual supplied with your system. Agilent InfinityLab LC Series VWD User Manual...

  • Page 64: Solvent Information

    Most data available refers to room temperature (typically 20 – 25 °C, 68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials. Agilent InfinityLab LC Series VWD User Manual...
  • Page 65 It has limited stability with aliphatic, aromatic and halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For normal phase applications, the maximum pressure should be limited to 200 bar. Agilent InfinityLab LC Series VWD User Manual...
  • Page 66 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. Agilent InfinityLab LC Series VWD User Manual...
  • Page 67 Gold Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia. Agilent InfinityLab LC Series VWD User Manual...
  • Page 68 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series VWD User Manual...

  • Page 69: Optimizing The Detector

    Optimizing the Detector Introduction Match the Flow Cell to the Column Set the Detector Parameters Warm up of the Detector This chapter provides information on how to optimize the detector. Agilent InfinityLab LC Series VWD User Manual...

  • Page 70: Introduction

    The information below will guide you on how to get the best detector performance. Follow these rules as a start for new applications. It gives a rule-of-thumb for optimizing the detector parameters. Agilent InfinityLab LC Series VWD User Manual...

  • Page 71: Match The Flow Cell To The Column

    Therefore we recommend to use the damper plus mixer for a highest signal to noise. • If longer columns (> 50 mm) for higher resolution are used, then the next larger flow cell is the preferred choice for higher sensitivity. Agilent InfinityLab LC Series VWD User Manual...
  • Page 72 As a rule-of-thumb, the flow cell volume should be about 1/3 of the peak volume at half height. To determine the volume of your peaks, take the peak width as reported in the integration results multiply it by the flow rate and divide it by 3). Agilent InfinityLab LC Series VWD User Manual...
  • Page 73 10/10 However you have to be aware that there is additional tolerance of gasket NOTE thickness and its compression ratio which is supposed to be very small in comparison with the machining tolerance. Agilent InfinityLab LC Series VWD User Manual...

  • Page 74: Set The Detector Parameters

    • at a wavelength with moderate absorptivity if you work with high concentrations, and • preferably where the spectrum is flat for better linearity. 3 Consider to use time-programming to further optimization. Agilent InfinityLab LC Series VWD User Manual...

  • Page 75: Warm Up Of The Detector

    The example below was done under stable environmental conditions. The figures below show the first two hours of a detector warm-up phase. The lamp was turned on immediately after turn on of the detector. Agilent InfinityLab LC Series VWD User Manual...
  • Page 76 Optimizing the Detector Warm up of the Detector Figure 22 Detector Warm-up – 1 hour Figure 23 Detector Warm-up – 2 hour Agilent InfinityLab LC Series VWD User Manual...

  • Page 77: Troubleshooting And Diagnostics

    Troubleshooting and Diagnostics Available Tests versus Interfaces Agilent Lab Advisor Software Overview about the troubleshooting and diagnostic features. Agilent InfinityLab LC Series VWD User Manual...

  • Page 78: Available Tests Versus Interfaces

    Preferred tool should be the Agilent Lab Advisor software, see “Agilent Lab Advisor Software” on page 79 • Screenshots used within these procedures are based on the Agilent Lab Advisor software. Figure 24 The Lab Advisor shows the available test Agilent InfinityLab LC Series VWD User Manual...

  • Page 79: Agilent Lab Advisor Software

    The optional Agilent Maintenance Wizard Add-on provides an easy-to-use, step-by-step multimedia guide for performing preventive maintenance on Agilent 1200 Infinity and Agilent InfinityLab LC Series instrument. The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files.

  • Page 80: Error Information

    Heater at fan assembly failed Heater Power At Limit Cover Violation This chapter describes the meaning of detector error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 81: What Are Error Messages

    In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent InfinityLab LC Series VWD User Manual...

  • Page 82: General Error Messages

    Check the logbook for the occurrence and source A not-ready condition was present during a of a not-ready condition. Restart the analysis sequence or multiple-injection run for a where required. period longer than the timeout threshold. Agilent InfinityLab LC Series VWD User Manual...

  • Page 83: Shutdown

    Check the vacuum degasser for an error condi- The degasser failed to generate sufficient tion. Refer to the Service Manual for the degasser vacuum for solvent degassing. or the pump that has the degasser built-in. Agilent InfinityLab LC Series VWD User Manual...

  • Page 84: Remote Timeout

    Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not rec- ognized by the system. Agilent InfinityLab LC Series VWD User Manual...

  • Page 85: Leak

    Please contact your Agilent service representa- Defective leak sensor. tive. Please contact your Agilent service representa- Leak sensor incorrectly routed, being tive. pinched by a metal component. Please contact your Agilent service representa- Power switch assembly defective tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 86: Leak Sensor Short

    Probable cause Suggested actions Please contact your Agilent service representa- Loose connection between the power switch tive. board and the main board Please contact your Agilent service representa- Defective power switch assembly tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 87: Compensation Sensor Short

    Probable cause Suggested actions Please contact your Agilent service representa- Fan cable disconnected. tive. Please contact your Agilent service representa- Defective fan. tive. Please contact your Agilent service representa- Defective main board. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 88: Open Cover

    The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions Reduce the load. The load on the ERI is too high. Agilent InfinityLab LC Series VWD User Manual...

  • Page 89: Detector Error Messages

    Top foam removed while lamp is on. tive. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service representa- Defective main board. tive. Please contact your Agilent service representa- Defective power supply. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 90: Uv Lamp: No Voltage

    Ensure the lamp is connected. Lamp disconnected. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service representa- Defective power supply. tive. Please contact your Agilent service representa- Defective main board. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 91: No Heater Current

    Fan not running (permitting lamp on). tive. Please contact your Agilent service representa- Defective main board. tive. Exchange the lamp. Defective or non-Agilent lamp. Please contact your Agilent service representa- Defective power supply. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 92: Wavelength Calibration Setting Failed

    Current step value too far from maximum. • Please contact your Agilent service represen- tative. Please contact your Agilent service representa- Misaligned/defective grating assembly. tive. Please contact your Agilent service representa- Defective main board. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 93: Wavelength Holmium Check Failed

    If defec- tive, please contact your Agilent service rep- resentative. • Run the grating-motor test to determine if the grating assembly is defective. If defec- tive, please contact your Agilent service rep- resentative. Agilent InfinityLab LC Series VWD User Manual...

  • Page 94: Grating Or Filter Motor Errors

    (656 nm) via the reference diode is performed. If the emission line is more than 3 nm away from 656 nm, the error message is generated. Probable cause Suggested actions Recalibrate the detector. Calibration incorrect. Agilent InfinityLab LC Series VWD User Manual...

  • Page 95: Cutoff Filter Doesn't Decrease The Light Intensity At 250 Nm

    Please contact your Agilent service representa- Cable/connector defective. tive. ADC Hardware Error Error ID: 7830, 7831 A/D-Converter hardware is defective. Probable cause Suggested actions Please contact your Agilent service representa- A/D-Converter hardware is defective. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 96: Illegal Temperature Value From Sensor On Main Board

    Replace the cable to the main board. The temperature sensor is defect. • Please contact your Agilent service represen- tative. Verify that the ambient conditions are within the Detector is exposed to illegal ambient condi- allowed range. tions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 97: Heater At Fan Assembly Failed

    0 means upper power limit hit (excessive ambient temperature drop). 1 means lower power limit hit (excessive ambient temperature increase). Probable cause Suggested actions Wait until temperature control equilibrates. Excessive ambient temperature change. Agilent InfinityLab LC Series VWD User Manual...

  • Page 98: Cover Violation

    Probable cause Suggested actions The top foam was removed during operation. Please contact your Agilent service representa- tive. Please contact your Agilent service representa- Foam not activating the sensor. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 99: Test Functions

    Interpreting the Results Holmium Oxide Test Failed D/A Converter (DAC) Test D/A Converter Test Evaluation Other Lab Advisor Functions EMFs - Early Maintenance Feature This chapter describes the detector’s built in test functions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 100: Introduction

    G7114A/B(Table 12 Advanced – FSE) page 101) Agilent ChemStation No tests available • Temperature main board Adding of temperature/lamp • Temperature optical unit signals to chromatographic sig- • Lamp anode voltage nals possible Agilent InfinityLab LC Series VWD User Manual...
  • Page 101 Basic Advanced - Spectral Scan Basic Advanced Controls - Advanced Method Parameters - Analog Output 1 Advanced Attenuation - D2 lamp required Advanced - Analog Output 1 Off- Advanced set [% Full Scale] Agilent InfinityLab LC Series VWD User Manual...
  • Page 102 - Identify Module Basic Advanced - Special Commands - Lamp tag required Basic Advanced - Cell tag required Basic Advanced - Clear Error Basic Advanced - Detector Reset Basic Advanced - Forced Cold Start Agilent InfinityLab LC Series VWD User Manual...
  • Page 103 Advanced - Reference Signal Advanced [mAU] - Board Temperature Advanced [°C] - Lamp Voltage [V] Advanced EMF Counters - Accumulated UV Basic Advanced Lamp On-Time - Number of UV Lamp Basic Advanced Ignitions Agilent InfinityLab LC Series VWD User Manual...
  • Page 104 Test Functions Introduction Figure 25 The Lab Advisor shows the available test Agilent InfinityLab LC Series VWD User Manual...

  • Page 105: Intensity Test

    Therefore, intensity spectra will differ slightly between instruments. The figure below shows a typical intensity test spectrum. The Intensity Test is available in Agilent Lab Advisor (preferred tool). Agilent InfinityLab LC Series VWD User Manual...

  • Page 106: Intensity Test Evaluation

    1 Run the Intensity-Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 26 Intensity Test – Results Figure 27 Intensity Test – Signal Agilent InfinityLab LC Series VWD User Manual...

  • Page 107: Intensity Test Failed

    Repeat the test with the flow cell window Flow cell windows dirty removed. If the test passes, exchange the flow cell windows. Please contact your Agilent service representa- Optics defect tive. Exchange the lamp. Defective lamp or optics. Agilent InfinityLab LC Series VWD User Manual...

  • Page 108: Cell Test

    Flush the flow cell with a flow of 1 mL/min for at least 10 minutes. Prerequisites 1 Run the Cell-Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 28 Test – Results Agilent InfinityLab LC Series VWD User Manual...

  • Page 109: Cell Test Failed

    Test Functions Cell Test Cell Test Failed Cell Test Failed Probable cause Suggested actions Flush flow cell Cell contaminated Clean/replace cell windows Cell windows are contaminated Check cell position Mechanical problem Agilent InfinityLab LC Series VWD User Manual...

  • Page 110: Wavelength Verification-Calibration

    VWM board, • at a regular interval, at least once per year (for example, prior to an Operational Qualification/Performance Verification procedure), and • when chromatographic results indicate the detector may require recalibration. Agilent InfinityLab LC Series VWD User Manual...
  • Page 111 1 Run the Wavelength Calibration with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 29 Wavelength Calibration - Results If you select No, the test is aborted. If you select Yes, the re-calibration is performed (the offset is corrected). Agilent InfinityLab LC Series VWD User Manual...

  • Page 112: Astm Drift And Noise Test

    1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 30 ASTM Drift and Noise Test – Results Figure 31 Drift and Noise Test – Signal Agilent InfinityLab LC Series VWD User Manual...

  • Page 113: Quick Noise Test

    In order to obtain reliable results, the lamp should be turned on for at least 10 minutes prior to measurement. 1 Run the Quick Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 32 Quick Noise Test – Results Agilent InfinityLab LC Series VWD User Manual...
  • Page 114 Test Functions Quick Noise Test Figure 33 Quick Noise Test – Signal Agilent InfinityLab LC Series VWD User Manual...

  • Page 115: Dark Current Test

    During the test, the shutter is moved into the light path. Next, the leakage current from both diodes is measured. 1 Run the Dark Current Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 34 Dark Current Test - Results Agilent InfinityLab LC Series VWD User Manual...

  • Page 116: Dark Current Test Failed

    Dark Current Test Failed Dark Current Test Failed Probable cause Suggested actions Replace optical unit. Defective sample or reference diode. Replace optical unit. Defective sample or reference ADC board. Exchange the main board. Defective main board. Agilent InfinityLab LC Series VWD User Manual...

  • Page 117: Holmium Oxide Test

    “Declaration of Conformity for HOX2 Filter” on page 220. NOTE When to do the Test • after recalibration, • as part of the Operational Qualification/Performance Verification procedure, • after flow cell maintenance or repair. Agilent InfinityLab LC Series VWD User Manual...

  • Page 118: Interpreting The Results

    1 Run the Holmium Oxide Test with the Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 36 Holmium Oxide Test - Results Figure 37 Holmium Oxide Test - Signals Agilent InfinityLab LC Series VWD User Manual...

  • Page 119: Holmium Oxide Test Failed

    OK, exchange the flow cell components. Run the holmium oxide filter test. If the test fails, Dirty or defective holmium oxide filter. contact your Agilent service representative. Please contact your Agilent service representa- Optical misalignment. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 120: D/A Converter (Dac) Test

    DAC test. When If the analog detector signal is noisy or missing. Preparations Lamp must be on for at least 10 minutes. Connect integrator, chart recorder or data system to the detector analog output. Agilent InfinityLab LC Series VWD User Manual...
  • Page 121 The test can be started via the command line. 1 To start the test TEST: DAC 1 Reply: RA 00000 TEST:DAC 1 2 To stop the test TEST:DAC 0 Reply: RA 00000 TEST:DAC 0 Agilent InfinityLab LC Series VWD User Manual...

  • Page 122: D/A Converter Test Evaluation

    The noise on the step should be less than 3 µV. Probable cause Suggested actions Check or replace the cable. Bad cable or grounding problem between detector and external device. Please contact your Agilent service representa- Defective detector main board. tive. Agilent InfinityLab LC Series VWD User Manual...

  • Page 123: Other Lab Advisor Functions

    Other Lab Advisor Functions Other Lab Advisor Functions EMFs - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. Figure 40 EMFs Agilent InfinityLab LC Series VWD User Manual...

  • Page 124: Maintenance

    Replace the Flow Cell / Cuvette Holder Repair the Flow Cells Using the Cuvette Holder Correcting Leaks Replace Leak Handling System Parts Replace the Module Firmware This chapter provides general information on maintenance of the detector. Agilent InfinityLab LC Series VWD User Manual...

  • Page 125: Introduction To Maintenance

    The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system. There are no serviceable parts inside. NOTE Do not open the module. Agilent InfinityLab LC Series VWD User Manual...

  • Page 126: Warnings And Cautions

    Repair work at the module can lead to personal injuries, e.g. shock hazard, when the cover is opened. Do not remove the cover of the module.  Only certified persons are authorized to carry out repairs inside the  module. Agilent InfinityLab LC Series VWD User Manual...
  • Page 127 C AU T I O N If you connect external equipment to the instrument, make sure that you  only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent InfinityLab LC Series VWD User Manual...

  • Page 128: Overview Of Maintenance

    A pressure tightness test should be done cleaning or exchange cell windows. after repair. Leak sensor drying If leak has occurred. Check for leaks. Leak handling system If broken or corroded. Check for leaks. replacement Agilent InfinityLab LC Series VWD User Manual...

  • Page 129: Cleaning The Module

    WAR N IN G shock hazard and damage the module Do not use an excessively damp cloth during cleaning.  Drain all solvent lines before opening any connections in the flow path.  Agilent InfinityLab LC Series VWD User Manual...

  • Page 130: Remove And Install The Doors

    The figures shown in this procedure exemplarily show the Infinity II Multisampler NOTE module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules. Agilent InfinityLab LC Series VWD User Manual...
  • Page 131 Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent InfinityLab LC Series VWD User Manual...

  • Page 132: Replace The Deuterium Lamp

    C AU T I O N (ESD). To prevent accidental electrostatic discharge when coming into contact  with components inside the instrument, touch one of the metal housing panels at the front of the instrument. Agilent InfinityLab LC Series VWD User Manual...
  • Page 133 Unplug the lamp connector. Unscrew the two lamp screws (Pozidriv). Remove the lamp and place it on a clean place. NOTE Do not touch the glass bulb with your fingers. It may reduce the light output. Agilent InfinityLab LC Series VWD User Manual...
  • Page 134 12 Turn the lamp ON and give the lamp more than 10 minutes to warm-up. 13 Perform a Wavelength Re-calibration after lamp warm-up. After lamp on, the detector requires a warm-up time of 60 min. No NOTE measurements should be performed during this time. Agilent InfinityLab LC Series VWD User Manual...

  • Page 135: Replace The Flow Cell / Cuvette Holder

    “Semi-micro Flow Cell (Parts)” on page 154 • “High Pressure Flow Cell (Parts)” on page 156 Preparations Turn the lamp OFF. Open the doors. Disconnect the inlet capillary and outlet tubing from the flow cell. Agilent InfinityLab LC Series VWD User Manual...
  • Page 136 Configure the flow cell. For further information see Agilent Information Center or the online help of your CDS. 10 Perform a Wavelength Verification-Calibration to check the correct position of the flow cell/cuvette holder. Agilent InfinityLab LC Series VWD User Manual...

  • Page 137: Repair The Flow Cells

    154 • “High Pressure Flow Cell (Parts)” on page 156 The shown cell parts will differ depending upon the flow cell type. For detailed NOTE parts schematics, refer to above mentioned pages. Agilent InfinityLab LC Series VWD User Manual...
  • Page 138 Maintenance Repair the Flow Cells Cell screw Conical springs Ring #1 PEEK Window Quartz Gasket #1 (small hole) RFID tag Gasket #2 (large hole) Ring #2 PEEK Agilent InfinityLab LC Series VWD User Manual...
  • Page 139 Otherwise tightening the cell screw might break the window. Conical springs Ring - Window - Gasket - Arrangement Figure 41 Orientation of conical springs e Screw the cell screw into the flow cell and tighten the screw. Agilent InfinityLab LC Series VWD User Manual...
  • Page 140 5 Reconnect the capillaries. 6 Perform a leak test. If OK, insert the flow cell. 7 Perform “Wavelength Verification-Calibration” on page 110 to check the correct positioning of the flow cell. 8 Replace the front cover. Agilent InfinityLab LC Series VWD User Manual...

  • Page 141: Using The Cuvette Holder

    If your own standard should be used to checkout the instrument. Parts required Description G1314-60200 Cuvette Holder Cuvette with the “standard”, e.g. NIST certified holmium oxide sample Preparations • Remove the normal flow cell. • Have cuvette with standard available. Agilent InfinityLab LC Series VWD User Manual...
  • Page 142 Clear side Light path Next Steps: Install the cuvette holder in the instrument. Perform your Wavelength Verification/Calibration (see “Wavelength Verification-Calibration” on page 110) to check the correct position of the cuvette holder. Agilent InfinityLab LC Series VWD User Manual...

  • Page 143: Correcting Leaks

    Wrench, 1/4 inch for capillary connections Open the doors. Use tissue to dry the leak sensor area [1]. Observe the capillary connections and the flow cell area [2] for leaks and correct, if required. Agilent InfinityLab LC Series VWD User Manual...
  • Page 144 Maintenance Correcting Leaks Close the doors. Agilent InfinityLab LC Series VWD User Manual...

  • Page 145: Replace Leak Handling System Parts

    Install the Leak Adapter by pressing it into the Main Cover. Insert the Tubing [2] (ca. 85 mm required for replace- ment) between Leak Adapter outlet and Leak Pan. Insert/close the doors. Agilent InfinityLab LC Series VWD User Manual...

  • Page 146: Replace The Module Firmware

    6.50 or above (main and resident). Other- wise the communication will not work. Conversion / emulation Possible via Lab Advisor software: • G7114B: G1314E/F • G7114A: G1314F Agilent InfinityLab LC Series VWD User Manual...

  • Page 147: Parts And Materials For Maintenance

    Standard Flow Cell 10 mm / 14 µl Micro Flow Cell 3 mm / 2 µl Semi-micro Flow Cell (Parts) High Pressure Flow Cell (Parts) Cuvette Holder (Parts) Accessory Kit This chapter provides information on parts for maintenance. Agilent InfinityLab LC Series VWD User Manual...

  • Page 148: Overview Of Maintenance Parts

    Micro flow cell 3 mm, 2 µL, 120 bar (with RFID tag) G1314-60183 Semi-micro flow cell 6 mm, 5 µL (with RFID tag) G1314-60182 High pressure flow cell 10 mm, 14 µL, 400 bar (with RFID tag) Agilent InfinityLab LC Series VWD User Manual...
  • Page 149 Overview of Maintenance Parts Item Description G1314-60023 Prep flow cell 0.06 mm G1314-60025 Prep flow cell 0.3 mm G1314-60024 Prep flow cell 3 mm 5062-8535 Waste accessory kit (Flow Cell to Waste) 5043-1013 Tubing Clip Agilent InfinityLab LC Series VWD User Manual...

  • Page 150: Standard Flow Cell 10 Mm / 14 Μl

    Screw for Clip, M2.2, 4.5 mm long G1314-44010 Clip for RFI ID tag G1314-65064 Gaskets #2 IN (small hole i.d. 1 mm), KAPTON 10/pk G1314-65066 Ring #2 kit (IN small hole, i.d. 1 mm) PEEK, 2/pk Agilent InfinityLab LC Series VWD User Manual...
  • Page 151 Parts and Materials for Maintenance Standard Flow Cell 10 mm / 14 µl 2 (3x) 2 (3x) Figure 44 Standard Flow Cell Agilent InfinityLab LC Series VWD User Manual...

  • Page 152: Micro Flow Cell 3 Mm / 2 Μl

    Cell repair kit semi-micro, includes window screw kit, Gasket Kit BACK, Gasket Kit FRONT and 4 mm hexagonal wrench 79883-68703 Window screw kit, includes 2 quartz windows, 2 compression wash- ers, 2 window holders, 2 window screws and 10 washers Agilent InfinityLab LC Series VWD User Manual...
  • Page 153 Parts and Materials for Maintenance Micro Flow Cell 3 mm / 2 µl 2 (5x) 2 (5x) Figure 45 Micro Flow Cell Agilent InfinityLab LC Series VWD User Manual...

  • Page 154: Semi-Micro Flow Cell (Parts)

    Semi-micro #1 gasket (long hole 1.5 x 3.5 mm), PTFE Semi-micro #2 gasket (long hole 2 x 4 mm), PTFE G1314-44010 Clip for RFI ID tag 0515-4780 Screw for Clip, M2.2, 4.5 mm long Agilent InfinityLab LC Series VWD User Manual...
  • Page 155 5 - Quartz window 6 - Semi-micro gasket #1 2 (3x) 7 - Semi-micro gasket #2 8 - Clip for RFI ID tag 9 - Screw for clip Figure 46 Semi-micro Flow Cell Agilent InfinityLab LC Series VWD User Manual...

  • Page 156: High Pressure Flow Cell (Parts)

    Screw for Clip, M2.2, 4.5 mm long 1 - Cell screw 2 - Ring PEEK 3 - Quartz window 4 - Gasket KAPTON 5 - RFID Clip 6 - Screw Figure 47 High Pressure Flow Cell Agilent InfinityLab LC Series VWD User Manual...

  • Page 157: Cuvette Holder (Parts)

    Parts and Materials for Maintenance Cuvette Holder (Parts) Cuvette Holder (Parts) For information the use of the cuvette holder, refer to “Using the Cuvette Holder” on page 141. Description G1314-60200 Cuvette Holder Figure 48 Cuvette Holder Agilent InfinityLab LC Series VWD User Manual...

  • Page 158: Accessory Kit

    5181-1516 CAN cable, Agilent module to module, 0.5 m 5500-1155 Tube Connector, 90 degree, ID 6.4 5043-1013 Tubing Clip (see item 7 in Figure 43 on page 148) 0100-1516 Fingertight fitting PEEK, 2/pk Agilent InfinityLab LC Series VWD User Manual...

  • Page 159: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables CAN Cable RS-232 Cables USB Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent InfinityLab LC Series VWD User Manual...

  • Page 160: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
  • Page 161 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series VWD User Manual...

  • Page 162: Analog Cables

    The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + Agilent InfinityLab LC Series VWD User Manual...
  • Page 163 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent InfinityLab LC Series VWD User Manual...

  • Page 164: Remote Cables

    POWER ON High grey NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown Agilent InfinityLab LC Series VWD User Manual...
  • Page 165 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Agilent InfinityLab LC Series VWD User Manual...
  • Page 166 High Ground Cable Shield- One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent InfinityLab LC Series VWD User Manual...
  • Page 167 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink Not connected Yellow Power on High Ready High Green Stop Black Start request Agilent InfinityLab LC Series VWD User Manual...

  • Page 168: Can Cable

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent InfinityLab LC Series VWD User Manual...

  • Page 169: Cables

    It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent InfinityLab LC Series VWD User Manual...

  • Page 170: Usb Cables

    To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series VWD User Manual...

  • Page 171: Hardware Information

    ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Instrument Layout Early Maintenance Feedback (EMF) This chapter describes the detector in more detail on hardware and electronics. Agilent InfinityLab LC Series VWD User Manual...

  • Page 172: Firmware Description

    APG/ERI remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent InfinityLab LC Series VWD User Manual...
  • Page 173 Main and resident firmware must be from the same set. Main FW update Main System Resident System Resident FW Update Figure 49 Firmware Update Mechanism Agilent InfinityLab LC Series VWD User Manual...
  • Page 174 G1314B to G1314C), the original feature set is available again. All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent InfinityLab LC Series VWD User Manual...

  • Page 175: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.

  • Page 176: Rear View Of The Module

    DE = Germany • JP = Japan • CN = China year and week of last major manufacturing change, e.g. 820 could be week 20 of 1998 or 2008 SSSSS real serial number Agilent InfinityLab LC Series VWD User Manual...
  • Page 177 CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambig- uously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent InfinityLab LC Series VWD User Manual...

  • Page 178: Interfaces

    Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 16 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers...
  • Page 179 • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent InfinityLab LC Series VWD User Manual...

  • Page 180: Overview Interfaces

    For details on USB refer to “USB (Universal Serial Bus)” on page 185. Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. Agilent InfinityLab LC Series VWD User Manual...
  • Page 181 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 182 PREPARE (L) Request to prepare for analysis (for example, calibration, detec- tor lamp on). Receiver is any module performing pre-analysis activi- ties. Special Interfaces There is no special interface for this module. Agilent InfinityLab LC Series VWD User Manual...

  • Page 183: Eri (Enhanced Remote Interface)

    ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal...
  • Page 184 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent InfinityLab LC Series VWD User Manual...

  • Page 185: Usb (Universal Serial Bus)

    Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent InfinityLab LC Series VWD User Manual...

  • Page 186: Setting The 6-Bit Configuration Switch

    For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Configuration switch Figure 52 Location of Configuration switch (example shows a G7114A/B VWD) Agilent InfinityLab LC Series VWD User Manual...
  • Page 187 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent InfinityLab LC Series VWD User Manual...

  • Page 188: Special Settings

     Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. Agilent InfinityLab LC Series VWD User Manual...
  • Page 189 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent InfinityLab LC Series VWD User Manual...

  • Page 190: Instrument Layout

    • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent InfinityLab LC Series VWD User Manual...

  • Page 191: Early Maintenance Feedback (Emf)

    The useful maintenance cycle is dependent on the requirements for use. Therefore, the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument. Agilent InfinityLab LC Series VWD User Manual...
  • Page 192 EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. This function is only available via Agilent Lab Advisor or Instant Pilot. NOTE Agilent InfinityLab LC Series VWD User Manual...

  • Page 193: Lan Configuration

    TCP/IP parameter configuration Configuration Switch Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet This chapter provides information on connecting the module to the Agilent ChemStation PC. Agilent InfinityLab LC Series VWD User Manual...

  • Page 194: What You Have To Do First

    PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. LAN interface MAC label Figure 54 Location of LAN interfaces and MAC label Agilent InfinityLab LC Series VWD User Manual...

  • Page 195: Tcp/Ip Parameter Configuration

    (short form ‘init mode’) defines how to determine the active TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 20 on page 197. Agilent InfinityLab LC Series VWD User Manual...

  • Page 196: Configuration Switch

    Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. NOTE Agilent InfinityLab LC Series VWD User Manual...

  • Page 197: Initialization Mode Selection

    The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Non-Volatile Active Parameter Stored Parameter Figure 56 Using Stored (Principle) Agilent InfinityLab LC Series VWD User Manual...
  • Page 198 In the Using Default mode, the parameters stored in the memory of the module NOTE are not cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent InfinityLab LC Series VWD User Manual...

  • Page 199: Dynamic Host Configuration Protocol (Dhcp)

    2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 200: Setup (Dhcp)

    Interface Card or Main Board). This MAC address is on a label on the card or at the rear of the main board, e.g. 0030d3177321. On the Local Controller the MAC address can be found under Details in the LAN section. Figure 59 LAN Setting on Instant Pilot Agilent InfinityLab LC Series VWD User Manual...
  • Page 201 Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 199). Agilent InfinityLab LC Series VWD User Manual...

  • Page 202: Manual Configuration

    Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it. TELNET Session Non-Volatile Stored Parameter Control Module Figure 60 Manual Configuration (Principle) Agilent InfinityLab LC Series VWD User Manual...

  • Page 203: With Telnet

    Handheld Controller, or the default IP address (see “Configuration Switch” on page 196). When the connection was established successfully, the module responds with the following: Figure 62 A connection to the module is made Agilent InfinityLab LC Series VWD User Manual...
  • Page 204 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent InfinityLab LC Series VWD User Manual...
  • Page 205 If the Initialization Mode Switch is changed now to “Using Stored” mode, the NOTE instrument will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent InfinityLab LC Series VWD User Manual...

  • Page 206: Appendix

    Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Solvent Information Declaration of Conformity for HOX2 Filter Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent InfinityLab LC Series VWD User Manual...

  • Page 207: General Safety Information

    Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 208: Before Applying Power

    Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 209: Do Not Operate In An Explosive Atmosphere

    WAR N IN G Personal injury (for example electrical shock, intoxication) Instruments that appear damaged or defective should be made  inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent InfinityLab LC Series VWD User Manual...

  • Page 210: Solvents

    For details, see the usage guideline for the solvent cabinet. A printed copy of the NOTE guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent InfinityLab LC Series VWD User Manual...

  • Page 211: Safety Symbols

    A pacemaker could switch into test mode and cause illness. A heart defibril- lator may stop working. If you wear these devices keep at least 55 mm dis- tance to magnets. Warn others who wear these devices from getting too close to magnets. Agilent InfinityLab LC Series VWD User Manual...
  • Page 212 C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. Do not proceed beyond a caution until you have fully understood and met  the indicated conditions. Agilent InfinityLab LC Series VWD User Manual...

  • Page 213: Refrigerant

    101.1 °C Critical pressure 40.6 bar Boiling point -26.5 °C Table 27 Physical properties of refrigerant R600a (isobutane) Molecular weight 58.12 Critical temperature 134.98 °C Critical pressure 36.6 bar Boiling point -11.7 °C Agilent InfinityLab LC Series VWD User Manual...
  • Page 214 Moreover, it must be noted that HFC-134a must always be extracted  from the system and collected. It must never be discharged into the atmosphere on environmental grounds (greenhouse effect). Agilent InfinityLab LC Series VWD User Manual...
  • Page 215 Dispose of properly in accordance with federal or local regulations.  Flammable Refrigerant Used.  Do not dispose of in domestic household waste. To return unwanted products, contact your local Agilent office, or see  http://www.agilent.com for more information. Agilent InfinityLab LC Series VWD User Manual...

  • Page 216: Waste Electrical And Electronic Equipment (Weee) Directive

    The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. Agilent InfinityLab LC Series VWD User Manual...

  • Page 217: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

  • Page 218: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) Agilent InfinityLab LC Series VWD User Manual...

  • Page 219: Solvent Information

    (e.g. EDTA), • mixtures of carbon tetrachloride with 2-propanol or THF. • Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride (PVDF), which is used in leak sensors, is not resistant to DMF. Agilent InfinityLab LC Series VWD User Manual...

  • Page 220: Declaration Of Conformity For Hox2 Filter

    Appendix Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter Agilent InfinityLab LC Series VWD User Manual...

  • Page 221: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Agilent InfinityLab LC Series VWD User Manual...
  • Page 222 • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2014-2019 Published in Germany 10/2019 G7114-90001* Part No: G7114-90001 Rev. C Document No: SD-29000240 Rev. C...

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