Page 1 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 2 (Computer Software) and, for the Depart- ment of Defense, DFARS 252.227-7015 (Technical Data - Commercial Items) and DFARS 227.7202-3 (Rights in Commer- cial Computer Software or Computer Software Documentation). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 3 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 4 This chapter describes the pump in more detail on hardware and electronics. LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC. Appendix This chapter provides addition information on safety, legal, and web. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 5: Table Of Contents
Setting up the Pump with the G4208A Instant Pilot Setting up the Pump with the Instrument Control Interface Solvent Information Algae Growth in HPLC Systems Prevent Blocking of Solvent Filters Normal Phase Applications Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 6 Exchange the Passive Inlet Valve Exchange the Outlet Valve Exchange the Purge Valve Frit or the Purge Valve Replace the O-Ring on the Purge Valve Remove the Pump Head Assembly Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 7 Hydraulic Path of the Quaternary Pump Hydraulic Path of the Isocratic Pump Identifying Cables Cable Overview Analog Cables Remote Cables CAN Cable Agilent Module to PC Agilent 1200 Module to Printer Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 8 Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) Manual Configuration Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 9: Introduction
How Does Variable Stroke Volume Work? Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the module, instrument overview, and internal connectors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 10: Introduction To The Pump
ASW and AIV. Degasser Outlet Valve Purge Valve Inlet Valve MCGV Pump Head Figure 1 Overview of the pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 11: G7110B Isocratic Pump
The Agilent 1260 Infinity II Isocratic Pump is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision in retention times is crucial. Status LED Purge valve Power switch Leak drain Figure 2 Overview of the pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 12: Features
PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 13: G7111A Quaternary Pump Vl
Status indicator Degasser Purge valve MCGV Power switch Leak drain Figure 3 Overview of the pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 14: Features
PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 15: G7111B Quaternary Pump
Status indicator Degasser Purge valve MCGV Power switch Leak drain Figure 4 Overview of the pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 16: Features
PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 17: G5654A Bio-Inert Pump
Buffer Advisor software allows fast pH scouting and easy buffer/solvent preparation in ion exchange chromatography. Status indicator Degasser Purge valve MCGV Power switch Leak drain Figure 5 Overview of the Bio-inert Pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 18: Features
Active seal wash and quaternary solvent blending included • Comes with Agilent Buffer Advisor software - for faster pH scouting and easy buffer/solvent preparation in ion exchange chromatography Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 19: Overview Of The Hydraulic Path
A damping unit is connected between the two piston chambers. A purge valve including a PTFE frit is fitted at the pump outlet for convenient priming of the pump head. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 20: Hydraulic Path
Hydraulic Path to sampling unit and column Seal wash pump Damper Purge valve Outlet valve Inlet valve to waste Figure 6 Hydraulic path of the isocratic pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 21 Seal wash pump Damper Degasser Purge valve Outlet valve Inlet valve to waste MCGV Figure 7 Hydraulic Path of the Quaternary Pump and Bio-inert Pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 22: How Does The Pump Work
The outlet of the purge valve assembly is then connected to the following chromatographic system. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 23 Inlet valve To waste From solvent bottle/ degasser/SSV or MCGV Seal Piston 1 Piston 2 Ball screw drive Gear Motor with encoder Figure 8 Principle of the Pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 24 “Performance Specifications Agilent 1260 Infinity II Quaternary Pump VL (G7111A)” on page 41 • Bio-inert pump: “Performance Specifications Agilent 1260 Infinity II Bio-inert Pump (G5654A)” on page 45 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 25: How Does Compressibility Compensation Work
By default, the stroke volume for the pump is set to AUTO mode. This means that the stroke is optimized for the flow rate in use. A change to larger stroke volumes is possible but not recommended. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 26: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 27 Introduction Leak and Waste Handling Figure 9 Infinity II Leak Waste Concept (Flex Bench installation) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 28 Introduction Leak and Waste Handling Figure 10 Infinity II Single Stack Leak Waste Concept (bench installation) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 29: Leak Sensor
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.  Do not use DMF as mobile phase. Check the leak sensor regularly for corrosion.  Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 30: Waste Concept
4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 31: Site Requirements And Specifications
Physical Specifications Agilent 1260 Infinity II Quaternary Pump (G7111B) Physical Specifications Agilent 1260 Infinity II Bio-inert Pump (G5654A) Performance Specifications This chapter provides information on environmental requirements, physical, and performance specifications. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 32: Site Requirements
Make sure the power connector of the instrument can be easily reached  and unplugged. Provide sufficient space behind the power socket of the instrument to  unplug the cable. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 33 WAR N IN G Solvents may damage electrical cables. Prevent electrical cables from getting in contact with solvents.  Exchange electrical cables after contact with solvents.  Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 34 If your module was shipped in cold weather, leave it in its box and allow it to  warm slowly to room temperature to avoid condensation. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 35: Physical Specifications
Safety standards: Overvoltage category II, For indoor use only IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 36 Safety standards: Overvoltage category II, For indoor use only IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 37 Safety standards: Overvoltage category II, For indoor use only IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 38 Safety standards: Overvoltage category II, For indoor use only IEC, EN, CSA, UL Pollution degree 2 ISM Classification ISM Group 1 Class B According to CISPR 11 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 39: Performance Specifications
Solvents with pH < 2.3 should not contain acids which attack stainless steel Integrated degassing unit Optional Number of channels: 2 Internal volume per channel: 1.5 mL Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 40 Housing All materials are recyclable Safety Data Sheet in SAP: 4093-3577 Rev. A For use with flow rates below 500 µL/min a vacuum degasser is required. NOTE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 41 1 mL/min (water/water with tracer) Composition range Settable: 0 – 100 % In 0.1 % increments Recommended: 5 – 95 % or 10 μL/min per channel, whichever is greater Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 42 Electronic records of maintenance and errors. Housing All materials are recyclable Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 43 Settable: 0 – 100 % In 0.1 % increments Recommended: 5 – 95 % or 10 μL/min per channel, whichever is greater, under recommended flow rate conditions Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 44 Electronic records of maintenance and errors Housing All materials are recyclable Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 45 Titanium, Gold, Platinum-Iridium, Sapphire, PEEK, PTFE, Ruby, Ceramic, FEP, PFA Active seal wash Included For solvent compatibility, refer to section "Solvent information" in the manual for the Agilent 1260 Infinity Bio-inert LC System. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 46: Using The Pump
Algae Growth in HPLC Systems How to Prevent and/or Reduce the Algae Problem Prevent Blocking of Solvent Filters Normal Phase Applications This chapter provides information for optimized usage of the module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 47: Magnets
Using the Pump Magnets Magnets Magnets in doors of pumps, autosamplers, detectors, and fraction collectors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 48: Turn On/Off
Using the Pump Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 49 Using the Pump Turn on/off Turn instrument On/Off with the control software. Power switch: Off Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 50: Status Indicators
5. Resident mode (blinking) - for example, during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 51: Best Practices
• Remove all samples from the sampler and store according to good laboratory practice. • Use recommended solvents to store the system. • Power off the system. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 52: Prepare The Pump
Use lower channels (A and/or D) for buffer solutions. • Regularly flush all MCGV channels with water to remove possible salt deposits. • Check compatibility of buffers and organic solvents to avoid precipitation. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 53: Leak And Waste Handling
Agilent Information Center or via the Internet. For details on correct installation, see separate installation documentation. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 54: Waste Concept
4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 55: Hints For Successful Use Of The Pump
“Maintenance of a Pump Head Without Seal Wash Option” on page 143). • Consider recommendations given in the solvent information section, see “Solvent Information” on page 65. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 56: Setting Up The Pump With The G4208A Instant Pilot
Pilot G4208A User's Guide (G4208-90006). Details about setting up module specific parameters can be found in the Instant Pilot online help. The pump parameters are described in detail in “Overview” on page 57. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 57: Setting Up The Pump With The Instrument Control Interface
IP address option and enter the appropriate value for the host computer to enable automatic detection of the hardware configuration. The system configures the instrument automatically with no further manual configuration necessary. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 58 This check box is marked to indicate that an optional seal wash has been detected during autoconfiguration. Please refer to the online help of your user interface for more detailed information. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 59: The Pump User Interface (Dashboard Panel)
The pressure setpoints. The red line shows the current maximum pressure limit; the gray area shows the current pressure (also shown as text). The current solvent flow rate (in mL/min) is displayed above the pressure display. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 60 Causes the LED on the front of the module to blink for a few seconds. Switch Pump On/Off Toggles the status of the pump, on or off. Bottle Fillings Displays the Bottle Fillings dialog box. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 61: Control Settings
Automatic Turn On Module can be turned on at a specified date/time. This feature can only be used if the module power switch is turned on. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 62: Method Parameter Settings
Timetable “Timetable Settings” on page 64 Advanced “Advanced Settings” on page 63 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 63 Using Automatic chooses the channel with the highest percentage at start conditions before a gradient. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 64 Change Solvent Composition - You can only use solvents, which have been enabled in the solvents section. • Function centric view - This checkbox allows you displaying parameter changes instead of a time table. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 65: Solvent Information
Piston seals/wash seals PTFE, SST (reversed phase) or UHMW-PE, SST (normal phase) Pressure sensor Purge valve SST, gold, PTFE, ceramic Damping unit SST, gold Capillaries/fittings Tubings PTFE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 66: Bio-Inert Materials
The flow downstream of the sample introduction contains no metals whatsoever. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 67 Do not use any parts that are not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see “General Information about Solvent/Material Compatibility” on page 68. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 68: General Information About Solvent/Material Compatibility
Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 69 PEEK capillaries keeps the flow path free of steel and ensures pressure stability up to 600 bar. If in doubt, consult the available literature about the chemical compatibility of PEEK. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 70 It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 71 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylenediaminetetraacetic acid). • Mixtures of carbon tetrachloride with isopropanol or THF. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 72 It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases, and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 73 Sapphire, ruby, and ceramics based on aluminum oxide Al are inert to almost all common acids, bases, and solvents. There are no documented incompatibilities for HPLC applications. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 74: Algae Growth In Hplc Systems
• If possible add a few mg/L sodium azide or a few percent organic solvent to the aqueous mobile phase. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 75: Prevent Blocking Of Solvent Filters
Place a layer of argon on top of your solvent. • Avoid exposure of the solvent bottle to direct sunlight. Never use the system without solvent filter installed. NOTE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 76: Normal Phase Applications
Do not use normal phase valves in applications running with aqueous  solutions. The N-Valves have been tested successfully in using hexane at pressures below 100 bar; heptane can be used as a substitute for neurotoxic hexane. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 77 1290 Infinity Quat Inlet Valve Type N (G4204-60122) Outlet valves Outlet Valve Type N/SFC 1290 Infinity Outlet Valve Type N (G1312-60167) (G4220-60128) Seals PE seals (pack of 2) (0905-1420) Wash Seal PE (0905-1718) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 78: Optimizing Performance
When to use the Seal Wash Function Choosing the Right Pump Seals Optimize the Compressibility Compensation Setting This chapter gives hints on how to optimize the performance or use additional devices. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 79: Using The Degasser
Optimizing Performance Using the Degasser Using the Degasser The quaternary pump has a built-in degasser, which should always be included to the flow path. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 80: Operational Hints For The Vacuum Degasser
(3 – 5 mL/min). Alternatively, a syringe can be used to draw the solvent through the (empty) degasser if the pump does not aspirate the solvent by itself. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 81: Operational Hints For The Multi Channel Gradient Valve (Mcgv)
The use of an Inline filter (G1311-60006) can avoid or reduce such effects by filtering crystals and re-dissolving them over time. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 82: When To Use The Seal Wash Function
This mixture prevents growth of algae or bacteria in the wash bottle and reduces the surface tension of the water. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 83: Choosing The Right Pump Seals
Polyethylene seals have a limited pressure range of 0 – 200 bar. When used NOTE above 200 bar their lifetime is reduced significantly. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 84: Optimize The Compressibility Compensation Setting
If ChemStation (classic view) is used and the ripple shown is positive, then the compressibility setting should be decreased. If it is negative, it should be increased. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 85 Carbon tetrachloride Chloroform Cyclohexane Ethanol Ethyl acetate Heptane Hexane Isobutanol Isopropanol Methanol 1-Propanol Toluene Water Please check section "Solvent Information" for compatibility to your specific LC system. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 86: Troubleshooting And Diagnostics
Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 87: User Interfaces
Table 19 Test functions available vs. user interface Test Instant Pilot G4208A Agilent Lab Advisor System Pressure Test Pump Leak Rate Test Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 88: Agilent Lab Advisor Software
The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 89: Error Information
SSV Protection circuit triggered This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 90: What Are Error Messages
In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 91: General Error Messages
A not-ready condition was present during a of a not-ready condition. Restart the analysis sequence or multiple-injection run for a where required. period longer than the timeout threshold. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 92: Shutdown
The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 93: Remote Timeout
Defective CAN cable. Switch off the system. Restart the system, and Defective mainboard in another module. determine which module or modules are not recognized by the system. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 94: Leak
If there are still signs of a leak, exchange the outlet valve. appropriate seal (purge valve, inlet valve, outlet valve). Exchange the pump seals. Defective pump seals. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 95: Leak Sensor Open
Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. Please contact your Agilent service Power switch assembly defective. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 96: Leak Sensor Short
Suggested actions Please contact your Agilent service Loose connection between the power switch representative. board and the mainboard. Please contact your Agilent service Defective power switch assembly. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 97: Compensation Sensor Short
Suggested actions Please contact your Agilent service Defective power switch assembly. representative. Please contact your Agilent service Loose connection between the power switch representative. board and the mainboard. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 98: Fan Failed
The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions Reduce the load. The load on the ERI is too high. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 99: Module Error Messages
(autosampler), column frits and capillaries with small internal diameters (e.g. 50 µm ID). Please contact your Agilent service Defective damper. representative. Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 100: Pressure Below Lower Limit
-120 mV across the damper connector. Probable cause Suggested actions Please contact your Agilent service Damper disconnected. representative. Please contact your Agilent service Defective damper. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 101: Mcgv Fuse
Please contact your Agilent service Defective connection cable (front panel to representative. mainboard). Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 102: Valve Failed (Mcgv)
Please contact your Agilent service Connection cable (inside instrument) not representative. connected. Please contact your Agilent service Connection cable (inside instrument) representative. defective. Exchange the gradient valve. Gradient valve defective. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 103: Motor-Drive Power
Please contact your Agilent service Defective pump drive assembly. representative. Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 104: Servo Restart Failed
Exchange the (passive or active) inlet valve. Blocked (passive or active) inlet valve. Please contact your Agilent service Defective pump drive assembly. representative. Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 105: Pump Head Missing
Irregular or sticking drive movement. pistons, and internal components for signs of wear, contamination or damage. Exchange components as required. Please contact your Agilent service Defective pump drive assembly. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 106: Index Adjustment
Probable cause Suggested actions Please contact your Agilent service Disconnected or defective encoder cable. representative. Please contact your Agilent service Defective pump drive assembly. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 107: Initialization Failed
Exchange the (passive or active) inlet valve. Blocked (passive or active) inlet valve. Please contact your Agilent service Defective pump drive assembly. representative. Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 108: Wait Timeout
Exchange defective capillaries. Ensure correct operating condition for the Flow changed after starting test. special application in use. Please contact your Agilent service Defective pump drive assembly. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 109: Degasser: Signal Fail
Liquid in degasser tubing. representative. Please contact your Agilent service Leak in degasser tubing or chamber. representative. Please contact your Agilent service Degasser vacuum pump defective. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 110: Degasser: Limit Not Reached
Check if the correct valve is connected to the Wrong valve connected. correct port. Change the Solvent Selection Valve. Defective Solvent Selection Valve. Please contact your Agilent service Defective mainboard. representative. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 111: Test Functions And Calibration
Potential Causes of System Pressure Test Failure Leak Rate Test Running the Test Evaluating the Results Potential Causes of Leak Rate Test Failure This chapter describes the tests for the module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 112: Introduction
Test Functions and Calibration Introduction Introduction Following tests are available in Lab Advisor: • System Pressure Test • Leak Rate Test All four pumps are available with LabAdvisor B.02.08. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 113: System Pressure Test
Using a degasser is highly recommended. Then the flow path is blocked by a blank nut at any position between the purge valve and the column outlet. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 114 A leak rate smaller than 3 µL/min is good enough for operating the pump reliably. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 115: Running The Test
“Evaluating the Results” on page 116 describes the evaluation and interpretation of the System Pressure Test results. For detailed instructions refer to the Agilent Lab Advisor software. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 116: Evaluating The Results
Exclude each module one by one to determine which module is leaking. If the pump is determined to be the source of the leak, run the Pump Leak Rate Test. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 117: Potential Causes Of System Pressure Test Failure
Autosampler: Needle seat. Column compartment: Loose or leaky fitting. Tighten or exchange the fitting or capillary. Exchange the rotor seal. Column compartment: Rotor seal in optional valve. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 118: Leak Rate Test
Before the test, the pump is flushed with solvent in order to remove air bubbles, as air bubbles are compressed during the test and therefore would appear as leaks. Using a degasser is highly recommended. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 119 1. For a binary pump (G1312B/C, G7112B, K1312B, G4782A), the test is run for both pump heads for channels A and B. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 120: Running The Test
1 Select the Leak Rate Test from the Test Selection menu. 2 Start the test and follow the instructions. Make sure to release the pressure by slowly opening the purge valve when the NOTE test has finished. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 121: Evaluating The Results
If any of the leak rates exceeds 3 µL/min, the test will fail. to column Damper Outlet valve Purge valve Inlet valve to waste Seals Pump chamber 1 Pump chamber 2 Piston 1 Piston 2 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 122: Potential Causes Of Leak Rate Test Failure
Inspect piston, replace piston and/or seal Leak at piston 1 or seal in chamber 1 Replace inlet valve or inlet valve cartridge (AIV Leak at inlet valve only) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 123 Probable cause Suggested actions Replace the part which has failed and re-run the Leak at outlet valve test. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 124: Maintenance
Exchange the Active Inlet Valve (AIV) or its Cartridge Exchange the Seal Wash Cartridge Replace Leak Handling System Parts Replacing the Module Firmware This chapter describes the maintenance of the module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 125: Introduction To Maintenance And Repair
The module is designed for easy repair. The most frequent repairs such as piston seal change and purge valve frit change can be done from the front of the module with the module in place in the system stack. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 126: Warnings And Cautions
If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 127: Overview Of Maintenance And Repair
Exchange pistons, see “Maintenance of a If scratched Seal life time shorter than usual - check pistons Pump Head Without Seal Wash Option” while changing the seals page 143 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 128: Cleaning The Module
Drain all solvent lines before opening any connections in the flow path.  A solution of 70 % isopropanol and 30 % water might be used if the surface of the NOTE module needs to be disinfected. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 129: Remove And Install Doors
The figures shown in this procedure exemplarily show the Infinity II Multisampler NOTE module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 130 For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 131: Checking And Replacing The Solvent Filter
(hydrostatic pressure). If the solvent filter is partly blocked only very little solvent will drip out of the solvent tube. 1 Remove the solvent filter from the inlet filter adapter and replace it by a new one. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 132: Exchange The Passive Inlet Valve
Using a 14 mm wrench loosen the passive inlet valve and remove the valve from the pump head. NOTE Beware of leaking solvents due to hydrostatic pressure. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 133 Insert the new valve into the pump head and tighten the Reconnect the solvent inlet tube to the passive inlet valve using a torque wrench (approx. 10 Nm). valve. approx. 10 Nm Close the doors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 134: Exchange The Outlet Valve
G1312-60167 Outlet valve (type N/SFC) G5611-60067 Outlet Valve (Bio-Inert) Preparations • Switch off the pump at the main power switch. • Open the doors of the module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 135 Reinstall the outlet valve and tighten it using a torque Reconnect the valve capillary to the outlet valve. wrench set to approx. 10 Nm. approx. 10 Nm Close the doors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 136: Exchange The Purge Valve Frit Or The Purge Valve
Switch off the pump at the main power switch. • Open the doors of the module. • Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 137 (slit in frit points to the front). NOTE Seal cap Before reinstallation always check the gold seal in the seal cap. A deformed seal cap should be exchanged. Frit Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 138 Anticipate the correct position of the connections before tightening the valve. Insert the purge valve into the pump head and fix it with a 14 mm wrench. Close the doors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 139: Replace The O-Ring On The Purge Valve
• Remove the purge valve from the pump head. Disassemble the purge valve. Remove the old o-ring from the purge valve. Clean the purge valve parts. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 140 Reinstall the seal cap including the gold seal. pump. The o-ring will take damage. NOTE Before reinstallation always check the gold seal in the seal cap. A deformed seal cap should be exchanged. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 141: Remove The Pump Head Assembly
Using a 1/4 inch wrench disconnect the valve capillary Disconnect the solvent inlet tube from the inlet valve. from the outlet valve. NOTE Beware of leaking solvents due to hydrostatic pressure. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 142 Remove the capillary at the bottom of the pump head. Using a 4 mm hexagonal key, stepwise loosen the two pump head screws. Remove the pump head from the pump drive. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 143: Maintenance Of A Pump Head Without Seal Wash Option
Place the pump head on a flat surface. Loosen the lock While holding the lower half of the assembly (piston screw (two turns). housing), carefully pull the pump housing away from the piston housing. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 144 Using the plastic side of the insert tool, insert new seals Place the support rings on the piston housing. Note the into the pump head. correct position of the pins. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 145 10 Insert the pistons and carefully press them into the seals. 11 Tighten the lock screw. 12 Install the pump head, see “Reinstall the Pump Head Assembly” on page 151. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 146: Maintenance Of A Pump Head With Seal Wash Option
Use an optional solvent shutoff valve or lift up solvent filters for avoiding leakages. • Remove the Pump Head Assembly. • Remove the wash solvent tubings from the support ring inlet and outlet. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 147 Check the piston surface and remove any deposits or layers: clean the piston surface with abrasive paper and rinse with 2-propanol. Replace piston if scratched. Piston Surface Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 148 10 Using the plastic side of the insert tool, press the new wash gasket and the wash seal from the support ring. wash seal (spring pointing upwards) into the recess of the support ring. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 149 Put the seal holder on top of the gasket. Backup seal Seal holder Seal wash gasket Seal holder Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 150 16 Insert the pistons and carefully press them into the seals. 17 Tighten the lock screw. 18 Install the pump head, see “Reinstall the Pump Head Assembly” on page 151. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 151: Reinstall The Pump Head Assembly
If needed, apply a small amount of grease on the back Slide the pump head assembly onto the pump drive. of the screws. Normally, the grease added during manufacturing is sufficient for a long time. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 152 Reconnect the solvent inlet tube to the passive inlet valve. Reconnect the outlet capillary and the waste tubing. If installed, reconnect the active inlet valve cable to its connector. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 153 154). 11 For the normal phase seal, the purge valve frit should be replaced, see “Exchange the Purge Valve Frit or the Purge Valve” on page 136. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 154: Seal Wear-In Procedure
7 Rinse your system with the solvent used for your next application. 8 Replace the purge valve frit, see “Exchange the Purge Valve Frit or the Purge Valve” on page 136. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 155: Exchange The Multi-Channel Gradient Valve (Mcgv)
(A and D) and the organic solvent to one of the upper gradient valve ports. It is best to have the organic channel directly above the buffer channel (e.g., A - buffer, B - organic solvent). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 156 Press the lower sides of the cover to unclip it. Remove Disconnect the MCGV cable from its connector. the cover. Unscrew the two screws and remove them. Remove the MCGV. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 157 11 Reconnect the tube from the inlet valve to the middle 12 Connect solvent tubes for channels A-D from the MCGV position of the MCGV. to the degasser outlets. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 158: Replace The Solvent Selection Valve (Isocratic Pump)
Using a screwdriver, loosen the holding screws of the Carefully disconnect the valve cable at the SSV valve holder. connector on the Z-Panel. Using a screwdriver, loosen the holding screws of the degasser front cover. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 159 After an exchange of the valve it may be required to pump several mL of solvent before the flow stabilizes at a pressure ripple as low as it used to be when the system was still working properly. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 160: Exchange The Active Inlet Valve (Aiv) Or Its Cartridge
Switch off pump at the main power switch and unplug the power cable. • Use an optional solvent shutoff valve or lift up solvent filters in solvent reservoirs for avoiding leakages. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 161 12 Reconnect the solvent inlet tube to the adapter. Reconnect the active inlet valve cable to the connector in the Z-panel. 13 Purge the system with 30 mL of solvent in order to achieve a low pressure ripple. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 162: Exchange The Seal Wash Cartridge
Connect the peristaltic pump tubes to the support rings the pump motor and push the plastic clips into the outlet and to the adapter leading to the waste bottle. module housing. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 163: Replace Leak Handling System Parts
3 Install the Leak Adapter by pressing it into the Main Cover. 4 Insert the Tubing (approximately 85 mm required for replacement) between Leak Adapter outlet and Leak Panel. 5 Close the doors. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 164: Replacing The Module Firmware
Agilent web. https://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 165: Parts For Maintenance
HPLC System Tool Kit Solvent Cabinet Bottle Head Assembly Hydraulic Path of the Quaternary Pump Hydraulic Path of the Isocratic Pump This chapter provides information on parts for maintenance. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 166: Accessory Kits
Y Tube Connector ID 6.4 5500-1155 Tube Connector, 90 degree, ID 6.4 5043-1372 Tubing Connector Leak 3-1 5043-1373 Tubing Connector Leak Cap 0890-1195 Flexible sleeving 1.45 mm/2.5 mm, PTFE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 167 Tube Connector, 90 degree, ID 6.4 5043-1373 Tubing Connector Leak Cap 5043-1372 Tubing Connector Leak 3-1 5500-1169 Y Tube Connector ID 6.4 0890-1195 Flexible sleeving 1.45 mm/2.5 mm, PTFE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 168: Pump Head Assembly Without Seal Wash
G7111A (OPTIONAL) G1312-60067 Outlet valve G1312-60167 Outlet valve (type N/SFC) 5042-1303 Lock screw G7111-60061 Purge valve for G7110B, G7111A, and G7111B 0515-2118 Pump head screw (M5, 60 mm) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 169 The Pump Head 1200 SL without Seal Wash (G1312-60056) includes items 1-7, 10 and 12. For piston seals, see “Choosing the Right Pump Seals” on page 83. Figure 13 Pump head assembly without seal wash option Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 170: Pump Head Assembly With Seal Wash Option
Gasket, seal wash (pack of 6) 5042-8952 Seal holder G5611-60016 Seal Holder (Bio-inert) (including: Bio-inert seal keeper (G5611-26210), Backup Ring for Seal Holder (G4220-24013)) G1398A Active Seal Wash Option Upgrade (includes service) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 171 Outlet valve (type N/SFC) G5611-60067 Outlet Valve (Bio-Inert) G7111-60061 Purge valve for G7110B, G7111A, and G7111B G5611-60064 Purge valve for G5654A 0515-2118 Pump head screw (M5, 60 mm) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 172 1220/1260 Seal Wash Pump Assembly see note below The Pump head assembly with seal wash (G1312-60045) includes items 1-8, 10 and 13. Figure 14 Pump Head with Seal Wash Option Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 173: Outlet Valve
Outlet valve for G7110B, G7111A, and G7111B G5611-60067 Outlet Valve (Bio-Inert) for G5654A G1312-60167 Outlet Valve Type N/SFC for G7110B, G7111A, and G7111B Figure 15 Outlet valve Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 174: Purge Valve Assembly
G5611-60064 Purge valve for G5654A G7111-60061 Purge valve for G7110B, G7111A, and G7111B 01018-22707 PTFE frits (pack of 5) 5067-4728 Seal cap Figure 16 Purge Valve Assembly Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 175: Active Inlet Valve Assembly
Active Inlet Valve Cartridge (400 bar) G5611-60020 Cartridge for AIV (Bio-inert) G1311-67304 Connecting tube (needed for quaternary pump only) 0100-2298 Adapter, PEEK int. 1/4-28 to ext. 10-32 (needed for isocratic pump only) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 176 Parts for Maintenance Active Inlet Valve Assembly Active inlet valve cartridge Active inlet valve Figure 17 Active Inlet Valve Assembly Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 177: 1260 Infinity Ii Max Uptime Kit
Rheotool socket wrench ¼ inch 5500-1191 InfinityLab Quick Turn Capillary ST 0.12 mm x 280 mm, long socket G7167-68703 Fitting Intermediate Kit G7111-90120 Technical Note 1260 Infinity II Max Uptime Kit Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 178: 1260 Infinity Ii Max Uptime Kit Bio
Capillary PK/ST 0.17 mm x 500 mm, RLO/RLO (Bio-inert) 8710-1930 Plastic tubing cutter 8710-2391 Rheotool socket wrench ¼ inch G5654-90130 Technical Note 1260 Infinity II Max Uptime Kit Bio Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 179: 1260 Infinity Ii Low Dispersion Kit Mct
Capillary ST 0.12 mm x 500 mm SL/S G7116-60015 Quick Connect Heat Exchanger Standard G7167-68703 Fitting Intermediate Kit G7111-90130 Technical Note 1260 Infinity II Low Dispersion Kit MCT Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 180: Hplc System Tool Kit
0100-1710 (Mounting tool for flangeless nut) 0100-1681 (Adapter luer/barb) 5023-2499 (Hex Key Set) 01018-23702 (Seal Insert tool) 5043-1361 5067-6127 (Blank Nut V) (Hex Key Set Driver) 5023-2653 (Hex Key 3/32“) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 181: Solvent Cabinet
Parts for Maintenance Solvent Cabinet Solvent Cabinet Description 5067-6871 Solvent Cabinet Kit For details refer to: Usage Guideline for the Solvent Cabinet (01200-90150) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 182: Bottle Head Assembly
Tube screw (10/Pk) Wire marker 5062-2483 Tube PTFE 1.5 mm x 5 m, 3 mm od 5062-8517 Inlet filter adapter (4/Pk) 5041-2168 Solvent inlet filter, 20 µm pore size Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 183: Hydraulic Path Of The Quaternary Pump
Capillary Ti 0.17 mm x 500 mm, SL/SLV for G5654A 0100-1847 PEEK adapter 1/4-28 to 10-32 (Adapter AIV to solvent inlet tubes) (not shown) G1311-60006 Inline filter (not shown) (OPTIONAL) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 184 Parts for Maintenance Hydraulic Path of the Quaternary Pump Figure 18 Hydraulic Flow Path of the Quaternary Pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 185: Hydraulic Path Of The Isocratic Pump
Waste tube, 5 m (reorder pack) G7120-60007 Bottle Head Assembly (not shown) G1311-60006 Inline filter (not shown) (OPTIONAL) Figure 19 Hydraulic Flow Path of the Isocratic Pump Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 186: Identifying Cables
Remote Cables CAN Cable Agilent Module to PC Agilent 1200 Module to Printer This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 187: Cable Overview
5188-8057 Fraction Collection ERI remote Y-cable CAN cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 188 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 189: Analog Cables
Analog - Center Analog + Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 190 Identifying Cables Analog Cables Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 191: Remote Cables
The configuration for IO4 and IO5 is swapped for modules with firmware lower than D.07.10. Peak Detection is used for LCMS systems connected with the Fraction Collection NOTE Remote Y-Cable (5188-8057). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 192 D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 193 One end of these cables provides an Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 194 (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink Not connected Yellow Power on High Ready High Green Stop Black Start request Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 195: Can Cable
LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 196: Agilent Module To Pc
"Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 197: Agilent 1200 Module To Printer
To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 198: Hardware Information
USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Special Settings Instrument Layout Early Maintenance Feedback This chapter describes the pump in more detail on hardware and electronics. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 199: Firmware Description
• diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 200 Main and resident firmware must be from the same set. Main FW update Main System Resident System Resident FW Update Figure 20 Firmware Update Mechanism Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 201 All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • https://www.agilent.com/en-us/firmwareDownload?whid=69761 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 202: Electrical Connections
Personal injury or damage to the instrument Any other instruments connected to this instrument shall be approved to  a suitable safety standard and must include reinforced insulation from the mains. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 203: Rear View Of The Module
Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 204: Interfaces
(on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A, G7132A G7161A/B Samplers G7129A/B/C G7167A/B/C, G7137A, G5668A, G3167A G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 205 RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 206: Overview Interfaces
211. Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s mainboard. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 207 "low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5.0 V (with respect to the ground terminal). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 208 (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. Special Interfaces There is no special interface for this module. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 209: Eri (Enhanced Remote Interface)
(in and out) and small controllers, etc. Figure 22 Location of the ERI interface (example shows a G7114A/B VWD) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 210 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 211: Usb (Universal Serial Bus)
USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 212: Setting The 6-Bit Configuration Switch
For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Configuration switch Figure 23 Location of Configuration switch (example shows a G7114A/B VWD) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 213 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode Test, COM settings are taken from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 214: Special Settings
Save your methods and data before executing a forced cold start.  If you use the following switch settings and power the instrument up again, it will start as described above. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 215 Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 216: Instrument Layout
• the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 217: Early Maintenance Feedback
EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 218: Lan Configuration
General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet With the Instant Pilot (G4208A) This chapter provides information on connecting the module to the Agilent ChemStation PC. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 219: What You Have To Do First
PC network card using a crossover network cable (point-to-point) or • a hub or switch using a standard LAN cable. LAN interface MAC label Figure 25 Location of LAN interfaces and MAC label Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 220: Tcp/Ip Parameter Configuration
TCP/IP parameters after power-on. The parameters may be derived non-volatile memory or initialized with known default values. The initialization mode is selected by the configuration switch, see Table 26 on page 222. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 221: Configuration Switches
The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. NOTE Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 222: Initialization Mode Selection
The TCP/IP connection will be established using these parameters. The parameters were configured previously by one of the described methods. Non-Volatile Active Parameter Stored Parameter Figure 27 Using Stored (Principle) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 223 In the Using Default mode, the parameters stored in the memory of the module NOTE are not cleared automatically. If not changed by the user, they are still available, when switching back to the mode Using Stored. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 224: Dynamic Host Configuration Protocol (Dhcp)
2 It may be necessary to fully qualify the hostname with the DNS suffix, e.g. 0030d3177321.country.company.com. 3 The DHCP server may reject the hostname proposed by the card and assign a name following local naming conventions. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 225 0030d3177321. On the Local Controller the MAC address can be found under Details in the LAN section. Figure 30 LAN Setting on Instant Pilot Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 226 Advisor, Firmware Update Tool) and use MAC address as host name, e.g. 0030d3177321. The LC system should become visible in the control software (see Note in section “General Information (DHCP)” on page 224). Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 227: Manual Configuration
TELNET Session Non-Volatile Stored Parameter Control Module Figure 31 Manual Configuration (Principle) Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 228: With Telnet
(see “Configuration Switches” on page 221). When the connection was established successfully, the module responds with the following: Figure 33 A connection to the module is made Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 229 Then press [Enter], where parameter refers to the configuration parameter you are defining, and value refers to the definitions you are assigning to that parameter. Each parameter entry is followed by a carriage return. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 230 If the Initialization Mode Switch is changed now to “Using Stored” mode, the NOTE instrument will take the stored settings when the module is re-booted. In the example above it would be 192.168.254.12. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 231: With The Instant Pilot (G4208A)
5 Press the Edit button (only visible if not in Edit mode), perform the required changes and press the Done button. 6 Leave the screen by clicking Exit. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 232: Appendix
Safety Symbols Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides addition information on safety, legal, and web. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 233: General Safety Information
Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 234: Before Applying Power
Any interruption of the protective (grounding) conductor or disconnection of the protective earth terminal will cause a potential shock hazard that could result in personal injury. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 235: Do Not Operate In An Explosive Atmosphere
Personal injury (for example electrical shock, intoxication) Instruments that appear damaged or defective should be made  inoperative and secured against unintended operation until they can be repaired by qualified service personnel. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 236: Solvents
For details, see the usage guideline for the solvent cabinet. A printed copy of the NOTE guideline has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 237: Safety Symbols
If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 238 Do not proceed beyond a caution until you have fully understood and met  the indicated conditions. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 239: Waste Electrical And Electronic Equipment (Weee) Directive
Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see https://www.agilent.com for more information. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 240: Radio Interference
If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 241: Sound Emission
Sound Emission Sound Emission Sound pressure Sound pressure Lp <70 db(A) according to DIN EN ISO 7779 Schalldruckpegel Schalldruckpegel Lp <70 db(A) nach DIN EN ISO 7779 Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 242: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: https://www.agilent.com Agilent InfinityLab LC Series 1260 Infinity II Isocratic and Quaternary Pumps User Manual...
Page 243 • using the pump, • optimizing performance, • troubleshooting and diagnostics, • maintenance, • parts and materials for maintenance, • identifying cables, • hardware information, • appendix. www.agilent.com  Agilent Technologies Inc. 2016-2023 Edition: 07/2023 Document No: SD-29000225 Rev. F...

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Agilent Technologies InfinityLab LC Series User Manual

Table of Contents

3 Using the Pump

4 Optimizing Performance

6 Error Information

7 Test Functions and Calibration

8 Maintenance

9 Parts for Maintenance

10 Identifying Cables

11 Hardware Information

12 LAN Configuration

13 Appendix

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