General Recommendations When Working With Rna - Agilent Technologies 4150 TapeStation system System Manual

7
Good Measurement Practices
RNA Assays

General Recommendations When Working with RNA

When working with RNA samples, make sure to consider following
recommendations:
• Always follow the GLP-rules established in the laboratory
• Wear gloves all the times to prevent contamination with RNases
• Thaw RNA samples and ladder on ice and keep them on ice during sample
• Ensure all working areas and plastic ware are RNase free
Not following these general guidelines might result in RNA degradation and false data.
N O T E
Figure 26
100
preparation
Example of a degraded RNA sample in the High Sensitivity RNA assay
resulting in missing peak identification in the Gel View (Left) and
Electropherogram View (Right) of the TapeStation Analysis software.
Agilent 4150 TapeStation System Manual