Gassing Cell Culture System - Infors HT Multifors 2 Operating Manual

3.5.7 Gassing Cell Culture System

28 March 2018
Basically, the entire gas entry takes place via the sparger.
Additionally, head space gassing with air is possible, too. This pre-
vents from accumulation of CO
foam.
Optionally, CO gassing can be used for pH control instead of liq-
2
uid acid. Gas entry also takes place via sparger.
The specific gassing rate, calculated for the max. working volume
is for each vessel size and each variant 0.1 min
For head space gassing, this rate is 1 min
and variant.
Variant Standard
Gas(es) Gas flow
control
Air 1 MFC
Air + O 1 MFC
2
Air + N 1 MFC
2
Air + O + N 1 MFC
2 2
Variant High End
Gas Gas flow control
Air 1 MFC
Gases Gas flow control and gas
mix control
Air + O 2 MFC
2
Air + N 2 MFC
2
Air + O + N 3 MFC
2 2
Head space gassing (Air) & CO
Gas Gas flow con-
trol
Air Rotameter
Rotameter
CO
2
1 MFC
1 ) same MFC (mass flow controller) as MFC for gas flow
Multifors 2 - Parallel Bench-top Bioreactor
Technical Data
in the head room and reduces
2
.
-1
for each vessel size
-1
Accuracy Gas mix control
MFC
± 0.3 % ----
(final value)
2 solenoid valves
± 0.5 %
2 solenoid valves
(measured
3 solenoid valves
value)
Accuracy MFC
± 0.3 %
(final value)
± 0.5 %
(measured value)
sparger gassing (pH control)
2
Accuracy Gas mix control
Rotameter / MFC
± 4 % ----
± 4 % 1 solenoid valve
± 1 % 1 MFC
1
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