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Labfors 5
Infors HT Labfors 5 Manuals
Manuals and User Guides for Infors HT Labfors 5. We have
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Infors HT Labfors 5 manuals available for free PDF download: Operating Manual, User Instructions
Infors HT Labfors 5 Operating Manual (208 pages)
Bench-Top Bioreactor - Version for Microorganisms
Brand:
Infors HT
| Category:
Laboratory Equipment
| Size: 6.97 MB
Table of Contents
Table of Contents
3
1 General Information
11
About this Manual
11
Explanation of Special Notices
11
Warning Notices
11
Other Notices
12
Equipment Identification (Standard Identification Plate)
12
Declaration of Conformity
13
Customer Service and Services
13
2 Safety and Responsibility
14
Intended Use, Incorrect Use and Misuse
14
Qualified Personnel
15
Provider
15
User
15
Operator
16
Unauthorised Persons
17
Responsibility of the Provider
17
General Hazards
17
Electrical Current
18
Unauthorised Spare Parts and Accessories
18
Particular Hazards
18
Hot Surfaces
19
Dangerous Gases
19
Flammable or Explosive Substances
19
Corrosive or Toxic Substances
19
Bioactive Substances or Pathogenic Organisms
20
Overpressure or Vacuum
20
Warning Symbols on the Equipment
20
Declaration of Decontamination
20
3 Setup and Function
22
Basic Unit
22
Main Switch
23
Pumps
23
Identification Plate
24
Mains Connection
24
Water Connections
25
Gas Connections
25
Signal Connections
26
Connections for Single or Double Walled Vessels
26
Connections for Single Walled Vessels
27
Connections for Double Walled Vessels
28
Gassing Connection (Sparger)
29
Sensor Connections (Sensor Cables)
29
Connections and Valve for Water Flow Regulation of the Exit Gas Cooler
30
Operating Panel
31
Monitor Keys
31
Operating Panel Connections
32
Culture Vessels
33
Double Walled Culture Vessels
33
Single Walled Culture Vessels
34
Top Plate
35
Ports in the Vessel Top Plate and Their Configuration
35
Top Plate DN 115
36
Top Plate DN 150
37
Top Plate DN 200
38
Temperature Control System
38
Water Temperature Control
39
Temperature Control with Heater Pad and Cold Finger
39
Stirrer
40
Gassing System
41
Gassing Strategy
42
Gasmix System
42
Gas Entry
42
Exit Gas
42
Ph Control
43
Measurement System
43
Calibration
44
Mounting of the Sensor
44
Po Control
44
Measuring System
45
Measurement and Calibration
45
Mounting of the Sensor
46
Antifoam Control
46
4 Options
47
Pump(S)
47
Servomotor (High Torque)
47
Level Measurement
49
Exit Gas Analysis
50
Measuring Systems (Gas Sensors)
50
Connecting the Gas Sensors
50
January
50
Calibration
52
Replacing the Bluevary Gas Sensor Cartridge
52
Multiplexer (Gas Switching Module)
52
Turbidity Measurement
53
Sensors
53
Calibrating the Zero Point
54
Mounting the Sensor
54
Cleaning and Storing the Sensor
55
Interferences Turbidity Measurement
55
Specifications
56
Permissive Measurement
56
Redox Measurement
57
Measuring Systems (Sensors)
57
Calibration
57
Mounting the Sensor
58
Pco Measurement
58
Pressure Control
59
Labcip, CIP/SIP Unit
61
5 Accessories
63
Base Tray
64
Reagent Bottle and Pump Holder
64
Sampling System Super Safe Sampler
66
Sparger
68
Impellers
70
Pocket for Temperature Sensor (Pt100)
70
Baffles
71
Lock Nut
72
Blanking Plugs
73
Addition Port Adapters
74
Inoculation Needles
75
Septum Collars
78
Dip Tubes
79
Clamping Adapters
81
Electrode Holder
83
Threaded Adapter
83
Adapter
83
Heater Pad
84
Cold Finger
85
Exit Gas Cooler
87
Reagent Bottles
89
Antifoam Sensor
91
Pump Heads
91
Sterile Filters
92
O-Rings and Gaskets
93
Hoses and Accessories
94
Inoculation Accessories and Tools
95
6 Transport and Storage
96
Transport
96
Storage
97
7 Installation and Initial Operation
98
General Location Requirements for Installation
98
Minimum Distances
99
Power Supply
99
Water Supply and Return
99
Gas Supply
100
Exit Gas
101
Test Run
101
Preparation Test Run
101
Filling the Water Circuit
104
Stirring
105
Heating and Adjusting Temperature
105
Gassing
106
End of Test
106
8 Before Fermentation/Cultivation
108
Preparing and Autoclaving the Culture Vessel
108
Checking Gaskets (O-Rings)
108
Mounting the Impellers
109
Mounting Dip Tubes and Spargers
110
Mounting the Baffles
111
Moistening/Filling the Culture Vessel
112
Fitting the Vessel Top Plate
113
Mounting a Threaded Adapter
114
Mounting the Blanking Plugs
115
Mounting Addition Port Adapters
116
Mounting the Pocket for Temperature Sensor (Pt100)
117
Preparing an Inoculation Needle
117
Preparing the Dip Tube/Addition Port Adapter for Inoculation
118
Equipping Port with Septum Collar and Septum for Inoculation
118
Mounting and Preparing the Exit Gas Cooler
119
January
119
Mounting the Cold Finger
121
Checking Lubrication of the Mechanical Seal
121
Preparing the Sensors
121
Calibrating the Ph Sensor
122
Mouting a Sensor into a 12 MM Port
123
Mounting Sensors with Electrode Holder
123
Mounting the Antifoam Sensor
126
Preparing the Super Safe Sampler
129
Mounting the Sparger Hose and the Inlet Air Filter
130
Preparing the Gassing Hose Line on the Basic Unit
131
Calibrating the Pumps
131
Preparing the Reagent Bottles, Pumps and Hoses
132
Sterile Hose Connections
135
Removing the Pump Heads
135
Checklist before Autoclaving
136
Autoclaving
137
Connecting the Culture Vessel and Preparing the Cultivation
139
Connecting a Single Walled Vessel
139
Connecting a Double Walled Culture Vessel
141
Mounting the Pump Heads
142
Filling the Reagent Hoses
142
Connecting the Gassing
143
Connecting the Exit Gas Cooler
144
Coupling the Motor
145
Filling the Culture Vessel
145
Inserting the Temperature Sensor (Pt100) into the Pocket
146
Connecting the Antifoam Sensor
146
Connecting the Ph Sensor
147
Connecting the Po Sensor
148
Polarising the Po Sensor (METTLER)
149
Calibrating the Po Sensor
149
Checking the Hoses and Hose Connections
150
9 Fermentation/Cultivation
151
Preparing the Medium
151
Sampling
152
Inoculation
155
Inoculation with Inoculation Needle
156
Inoculation with a Syringe
157
Inoculation Using Dip Tube / Addition Port Adapter
157
Harvest
158
Emptying the Culture Vessel
159
Emptying the Reagent Hoses
159
Switching off the Equipment
159
Autoclaving the Culture Vessel after Fermentation/Cultivation
160
10 Cleaning and Maintenance
162
Cleaning Agent and Disinfectant
162
Cleaning the Culture Vessel - Routine Cleaning
162
Removing the Vessel Top Plate and Accessories
164
Removing the Exit Gas Cooler
164
Removing the Sensors
164
Removing Hoses, Filters and Pump Heads
165
Removing Blanking Plugs
166
Removing Threaded Adapters
166
Removing the Inoculation Needle, Septum Collar and Septum
166
Removing Addition Port Adapters
167
Removing the Vessel Top Plate
167
Removing the Pocket for Temperature Sensor (Pt100)
169
Removing the Sparger and the Dip Tube(S)
169
Removing the Baffles
169
Removing the Impellers
170
Removing the Stirrer Shaft
170
Cleaning and Storing Individual Parts
171
Cleaning the Sensors
172
Cleaning the Hoses and Pump Heads
173
Cleaning the Super Safe Sampler
173
Cleaning the Exit Gas Cooler
174
Cleaning the Basic Unit and Operating Panel
174
Cleaning the Heater Pad
175
Maintenance Plan
175
January
175
Lubricating the Mechanical Seal
177
Decalcifying the Equipment
177
Decalcifying Equipment Via Vessel Jacket
179
11 Interferences
180
Interferences Basic Unit and Operating Panel
180
Interferences Drive System
181
Interferences Temperature Control System
181
Interferences Gassing System
183
Interferences Ph-System
184
Interferences Po
185
System
185
Interferences Antifoam or Level Sensor and Antifoam Pump
186
Interferences Feed and Pump
187
Returning for Repair
188
12 Disassembly and Disposal
189
Disassembly
189
Disposal
189
13 Technical Data
191
Dimensions 1 Unit
191
Dimensions Master Unit and Satellite Units
193
Dimensions of Culture Vessels in Vessel Holder
194
Weights (Net)
196
Connection Values
196
Electrical
196
Water in
196
Specifications
197
Water out
197
Gas(Es) in
197
Exit Gas
197
Operating Unit
197
Culture Vessel
198
Stirrer
198
Temperature
200
Gassing
201
Antifoam
202
Pumps
204
Sterile Filters
205
Heater Pads for Single Walled Vessels
206
Auxiliary Supplies
207
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Infors HT Labfors 5 Operating Manual (183 pages)
Bench-Top Bioreactor - Version for Solid Substrates and Enzymatic Bioprocesses
Brand:
Infors HT
| Category:
Laboratory Equipment
| Size: 6.05 MB
Table of Contents
Table of Contents
3
1 General Information
10
About this Manual
10
Explanation of Special Notices
10
Warning Notices
10
Other Notices
11
Equipment Identification (Standard Identification Plate)
11
Declaration of Conformity
12
Customer Service and Services
12
2 Safety and Responsibility
13
Intended Use, Incorrect Use and Misuse
13
Qualified Personnel
14
Provider
14
User
14
Operator
15
Unauthorised Persons
16
Responsibility of the Provider
16
General Hazards
16
Electrical Current
17
Unauthorised Spare Parts and Accessories
17
Particular Hazards
17
Hot Surfaces
18
Dangerous Gases
18
Flammable or Explosive Substances
18
Corrosive or Toxic Substances
18
Bioactive Substances or Pathogenic Organisms
19
Overpressure or Vacuum
19
Warning Symbols on the Equipment
19
Declaration of Decontamination
20
3 Setup and Function
21
Basic Unit
21
Main Switch
22
Pumps
22
Identification Plate
23
Mains Connection
23
Water Connections
24
Gas Connections
24
Signal Connections
25
Connections for Vessel
26
Gassing Connection (Sparger/Head Space)
27
Sensor Connections (Sensor Cables)
27
Connections and Valve for Water Flow Regulation of the Exit Gas Cooler
28
Operating Panel
29
Monitor Keys
29
Operating Panel Connections
30
Culture Vessel
31
Top Plate
32
Ports in the Vessel Top Plate and Their Configuration
33
Temperature Control System
34
Stirrer
34
Gassing System
37
Gasmix System
37
Gas Entry
37
Exit Gas
37
Ph Control
38
Measurement System
38
Calibration
39
Mounting of the Sensor
39
Po Control
39
Measuring System
40
Measurement and Calibration
41
Mounting of the Sensor
41
4 Options
41
Pump(S)
41
Servomotor (High Torque)
42
Exit Gas Analysis
44
Measuring Systems (Gas Sensors)
44
Connecting the Gas Sensors
44
Calibration
46
Replacing the Bluevary Gas Sensor Cartridge
46
Multiplexer (Gas Switching Module)
46
Permissive Measurement
47
Pressure Control
47
5 Accessories
51
Base Tray
51
Reagent Bottle and Pump Holder
52
Sampling System Super Safe Sampler
53
Sparger with Sterile Sheath
55
Impellers
57
Pocket for Temperature Sensor (Pt100)
58
Blanking Plugs
58
January
58
Addition Port Adapters
60
Inoculation Needles
61
Septum Collars
64
Probe Protection for Ph Sensor
65
Dip Tubes
66
Clamping Adapters
68
Electrode Holder
69
Threaded Adapter
70
Adapter
70
Exit Gas Cooler
71
Reagent Bottles
73
Pump Heads
75
Sterile Filters
75
O-Rings and Gaskets
77
Hoses and Accessories
77
Inoculation Accessories and Tools
78
6 Transport and Storage
79
Transport
79
Storage
80
7 Installation and Initial Operation
81
General Location Requirements for Installation
81
Minimum Distances
82
Power Supply
82
Water Supply and Return
82
Gas Supply
83
Exit Gas
84
Test Run
84
Preparation Test Run
84
Filling the Water Circuit
86
Stirring
87
Heating and Adjusting Temperature
87
Gassing
88
End of Test
88
8 Before Fermentation/Cultivation
90
Preparing and Autoclaving the Culture Vessel
90
Checking Gaskets (O-Rings)
90
Mounting the Impellers
91
Assembling and Mounting the Sparger
92
Moistening/Filling the Culture Vessel
94
Fitting the Vessel Top Plate
95
Mounting a Threaded Adapter
96
Mounting the Blanking Plugs
97
Mounting Addition Port Adapters
99
Mounting the Pocket for Temperature Sensor (Pt100)
100
Preparing an Inoculation Needle
100
Equipping Port with Septum Collar and Septum for Inoculation
101
Mounting and Preparing the Exit Gas Cooler
101
Checking Lubrication of the Mechanical Seal
103
Preparing the Sensors
103
Calibrating the Ph Sensor
104
Fitting a Ph Sensor with a Probe Protection
105
Mounting Sensors with Electrode Holder
105
Preparing the Super Safe Sampler
107
Mounting the Sparger Hose and the Inlet Air Filter
109
Mounting the Hose and Inlet Air Filter for Head Space Gassing
109
Preparing the Gassing Hose Line on the Basic Unit
110
Calibrating the Pumps
111
Preparing the Reagent Bottles, Pumps and Hoses
111
Sterile Hose Connections
114
Removing the Pump Heads
114
Checklist before Autoclaving
115
Autoclaving
117
Connecting the Culture Vessel and Preparing the Cultivation
118
Connecting the Culture Vessel
118
Mounting the Pump Heads
119
Filling the Reagent Hoses
119
Connecting the Gassing
120
Connecting the Exit Gas Cooler
121
Coupling the Motor
122
Filling the Culture Vessel
122
Inserting the Temperature Sensor (Pt100) into the Pocket
123
Connecting the Ph Sensor
124
Connecting the Po 2 Sensor
125
Sensor (METTLER)
126
Calibrating the Po
126
Sensor
126
Checking the Hoses and Hose Connections
127
9 Fermentation/Cultivation
128
Preparing the Medium
128
Sampling
129
Inoculation
133
Inoculation with Inoculation Needle
134
Inoculation with a Syringe
134
Inoculation Using Dip Tube / Addition Port Adapter
135
Extending the Sparger with the Sterile Sheath
135
Harvest
136
Emptying the Culture Vessel
137
Emptying the Reagent Hoses
137
Switching off the Equipment
137
Autoclaving the Culture Vessel after Fermentation/Cultivation
138
10 Cleaning and Maintenance
140
Cleaning Agent and Disinfectant
140
Cleaning the Culture Vessel - Routine Cleaning
140
Removing the Vessel Top Plate and Accessories
142
Removing the Exit Gas Cooler
142
Removing the Sensors
142
Removing Hoses, Filters and Pump Heads
143
Removing Blanking Plugs
144
Removing Threaded Adapters
144
Removing the Inoculation Needle, Septum Collar and Septum
144
Removing Addition Port Adapters
145
Removing the Vessel Top Plate
145
Removing the Pocket for Temperature Sensor (Pt100)
147
Removing the Sparger and the Dip Tube(S)
147
Removing the Impellers
149
Removing the Stirrer Shaft
149
Cleaning and Storing Individual Parts
150
Cleaning the Sensors
151
Cleaning the Hoses and Pump Heads
152
Cleaning the Super Safe Sampler
152
Cleaning the Exit Gas Cooler
153
Cleaning the Basic Unit and Operating Panel
153
Maintenance Plan
153
Lubricating the Mechanical Seal
155
Decalcifying the Equipment
155
Decalcifying Equipment Via Vessel Jacket
157
11 Interferences
158
Interferences Basic Unit and Operating Panel
158
Drive System Faults
159
Interferences Temperature Control System
159
Interferences Gassing System
161
Interferences Ph-System
162
Interferences Po System
163
Interferences Feed and Pump
164
Returning for Repair
165
12 Disassembly and Disposal
165
Disassembly
165
Disposal
166
13 Technical Data
167
Dimensions 1 Unit
167
Dimensions Master Unit and Satellite Units
169
Dimensions of Culture Vessels in Vessel Holder
170
Weights (Net)
172
Connection Values
172
Electrical
172
Water in
172
Water out
173
Gas(Es) in
173
Exit Gas
173
Specifications
173
Operating Unit
173
Culture Vessel
174
Stirrer
174
Impellers
175
Temperature
177
Gassing
178
Po 2
179
January
180
Sterile Filters
181
Auxiliary Supplies
182
Infors HT Labfors 5 User Instructions (32 pages)
Single-Use Vessel Option
Brand:
Infors HT
| Category:
Laboratory Equipment
| Size: 0.44 MB
Table of Contents
Table of Contents
3
1 General Information
5
About this Document
5
About the Device Option
5
2 Safety
6
Personnel
6
Personal Protective Equipment
7
3 Technical Data
8
Stirrer Drive
8
Temperature
8
4 Setup and Function
10
Overview Labfors 5 Cell with Single-Use Vessel Option
10
Single-Use Vessel
11
Heating and Temperature Measurement
11
Stirrer Drive
12
Connection with Gassing and Fluid Addition
13
Cultivation and Process Control
13
5 Procedures to Carry out Prior to Cultivation
14
Preparing the Reagent Bottles and Hoses
15
Connecting a Pump with a Reagent Bottle
15
Equipping a Reagent Bottle for Connection with a Fluid Inlet Line
17
Calibrating the Ph Sensor
18
Sterilising the Reagent Bottles and Sensors before Cultivation
18
Equipping the Gassing Line on the Equipment Side
20
Connecting the Reagent Bottles, Fitting the Sensors
20
Connecting the Sensors
20
Fitting and Connecting the Heater Pad
21
Connecting the Motor
23
Connecting the Gassing
24
Calibrating the Po Sensor
25
6 Cultivation
26
Safety
26
7 Procedures to Carry out after Cultivation
27
Emptying the Fluid Inlet Lines
27
Disconnecting Cable and Hose Connections, Removing the Sensors
28
Cleaning
31
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