Infors HT Labfors 5 Manuals

Manuals and User Guides for Infors HT Labfors 5. We have 2 Infors HT Labfors 5 manuals available for free PDF download: Operating Manual, User Instructions

Infors HT Labfors 5 Operating Manual (208 pages)

Bench-Top Bioreactor - Version for Microorganisms

Brand: Infors HT | Category: Laboratory Equipment | Size: 6 MB

Table of Contents
3
1 General Information

11
- About this Manual
  11
- Explanation of Special Notices
  11
  - Warning Notices
    11
  - Other Notices
    12
- Equipment Identification (Standard Identification Plate)
  12
- Declaration of Conformity
  13
- Customer Service and Services
  13
2 Safety and Responsibility

14
- Intended Use, Incorrect Use and Misuse
  14
- Qualified Personnel
  15
  - Provider
    15
  - User
    15
  - Operator
    16
- Unauthorised Persons
  17
- Responsibility of the Provider
  17
- General Hazards
  17
  - Electrical Current
    18
  - Unauthorised Spare Parts and Accessories
    18
- Particular Hazards
  18
  - Hot Surfaces
    19
  - Dangerous Gases
    19
  - Flammable or Explosive Substances
    19
  - Corrosive or Toxic Substances
    19
  - Bioactive Substances or Pathogenic Organisms
    20
  - Overpressure or Vacuum
    20
- Warning Symbols on the Equipment
  20
- Declaration of Decontamination
  20
3 Setup and Function

22
- Basic Unit
  22
  - Main Switch
    23
  - Pumps
    23
  - Identification Plate
    24
  - Mains Connection
    24
  - Water Connections
    25
  - Gas Connections
    25
  - Signal Connections
    26
  - Connections for Single or Double Walled Vessels
    26
    
    Connections for Single Walled Vessels
    27
    
    Connections for Double Walled Vessels
    28
  - Gassing Connection (Sparger)
    29
  - Sensor Connections (Sensor Cables)
    29
  - Connections and Valve for Water Flow Regulation of the Exit Gas Cooler
    30
- Operating Panel
  31
  - Monitor Keys
    31
  - Operating Panel Connections
    32
- Culture Vessels
  33
  - Double Walled Culture Vessels
    33
  - Single Walled Culture Vessels
    34
  - Top Plate
    35
  - Ports in the Vessel Top Plate and Their Configuration
    35
  - Top Plate DN 115
    36
  - Top Plate DN 150
    37
  - Top Plate DN 200
    38
- Temperature Control System
  38
  - Water Temperature Control
    39
  - Temperature Control with Heater Pad and Cold Finger
    39
- Stirrer
  40
- Gassing System
  41
  - Gassing Strategy
    42
  - Gasmix System
    42
  - Gas Entry
    42
- Exit Gas
  42
- Ph Control
  43
  - Measurement System
    43
  - Calibration
    44
  - Mounting of the Sensor
    44
- Po Control
  44
  - Measuring System
    45
  - Measurement and Calibration
    45
  - Mounting of the Sensor
    46
- Antifoam Control
  46
4 Options

47
- Pump(S)
  47
- Servomotor (High Torque)
  47
- Level Measurement
  49
- Exit Gas Analysis
  50
  - Measuring Systems (Gas Sensors)
    50
  - Connecting the Gas Sensors
    50
  - January
    50
  - Calibration
    52
  - Replacing the Bluevary Gas Sensor Cartridge
    52
- Multiplexer (Gas Switching Module)
  52
- Turbidity Measurement
  53
  - Sensors
    53
  - Calibrating the Zero Point
    54
  - Mounting the Sensor
    54
  - Cleaning and Storing the Sensor
    55
  - Interferences Turbidity Measurement
    55
  - Specifications
    56
- Permissive Measurement
  56
- Redox Measurement
  57
  - Measuring Systems (Sensors)
    57
  - Calibration
    57
  - Mounting the Sensor
    58
- Pco Measurement
  58
- Pressure Control
  59
- Labcip, CIP/SIP Unit
  61
5 Accessories

63
- Base Tray
  64
- Reagent Bottle and Pump Holder
  64
- Sampling System Super Safe Sampler
  66
- Sparger
  68
- Impellers
  70
- Pocket for Temperature Sensor (Pt100)
  70
- Baffles
  71
- Lock Nut
  72
- Blanking Plugs
  73
- Addition Port Adapters
  74
- Inoculation Needles
  75
- Septum Collars
  78
- Dip Tubes
  79
- Clamping Adapters
  81
- Electrode Holder
  83
- Threaded Adapter
  83
- Adapter
  83
- Heater Pad
  84
- Cold Finger
  85
- Exit Gas Cooler
  87
- Reagent Bottles
  89
- Antifoam Sensor
  91
- Pump Heads
  91
- Sterile Filters
  92
- O-Rings and Gaskets
  93
- Hoses and Accessories
  94
- Inoculation Accessories and Tools
  95
6 Transport and Storage

96
- Transport
  96
- Storage
  97
7 Installation and Initial Operation

98
- General Location Requirements for Installation
  98
- Minimum Distances
  99
- Power Supply
  99
- Water Supply and Return
  99
- Gas Supply
  100
- Exit Gas
  101
- Test Run
  101
  - Preparation Test Run
    101
  - Filling the Water Circuit
    104
  - Stirring
    105
  - Heating and Adjusting Temperature
    105
  - Gassing
    106
  - End of Test
    106
8 Before Fermentation/Cultivation

108
- Preparing and Autoclaving the Culture Vessel
  108
  - Checking Gaskets (O-Rings)
    108
  - Mounting the Impellers
    109
  - Mounting Dip Tubes and Spargers
    110
  - Mounting the Baffles
    111
  - Moistening/Filling the Culture Vessel
    112
  - Fitting the Vessel Top Plate
    113
  - Mounting a Threaded Adapter
    114
  - Mounting the Blanking Plugs
    115
  - Mounting Addition Port Adapters
    116
  - Mounting the Pocket for Temperature Sensor (Pt100)
    117
  - Preparing an Inoculation Needle
    117
  - Preparing the Dip Tube/Addition Port Adapter for Inoculation
    118
  - Equipping Port with Septum Collar and Septum for Inoculation
    118
  - Mounting and Preparing the Exit Gas Cooler
    119
  - January
    119
  - Mounting the Cold Finger
    121
  - Checking Lubrication of the Mechanical Seal
    121
  - Preparing the Sensors
    121
    
    Calibrating the Ph Sensor
    122
    
    Mouting a Sensor into a 12 MM Port
    123
    
    Mounting Sensors with Electrode Holder
    123
    
    Mounting the Antifoam Sensor
    126
  - Preparing the Super Safe Sampler
    129
  - Mounting the Sparger Hose and the Inlet Air Filter
    130
  - Preparing the Gassing Hose Line on the Basic Unit
    131
  - Calibrating the Pumps
    131
  - Preparing the Reagent Bottles, Pumps and Hoses
    132
  - Sterile Hose Connections
    135
  - Removing the Pump Heads
    135
  - Checklist before Autoclaving
    136
  - Autoclaving
    137
- Connecting the Culture Vessel and Preparing the Cultivation
  139
  - Connecting a Single Walled Vessel
    139
  - Connecting a Double Walled Culture Vessel
    141
  - Mounting the Pump Heads
    142
  - Filling the Reagent Hoses
    142
  - Connecting the Gassing
    143
  - Connecting the Exit Gas Cooler
    144
  - Coupling the Motor
    145
  - Filling the Culture Vessel
    145
  - Inserting the Temperature Sensor (Pt100) into the Pocket
    146
  - Connecting the Antifoam Sensor
    146
  - Connecting the Ph Sensor
    147
  - Connecting the Po Sensor
    148
  - Polarising the Po Sensor (METTLER)
    149
  - Calibrating the Po Sensor
    149
  - Checking the Hoses and Hose Connections
    150
9 Fermentation/Cultivation

151
- Preparing the Medium
  151
- Sampling
  152
- Inoculation
  155
- Harvest
  158
- Emptying the Culture Vessel
  159
- Emptying the Reagent Hoses
  159
- Switching off the Equipment
  159
- Autoclaving the Culture Vessel after Fermentation/Cultivation
  160
10 Cleaning and Maintenance

162
- Cleaning Agent and Disinfectant
  162
- Cleaning the Culture Vessel - Routine Cleaning
  162
- Removing the Vessel Top Plate and Accessories
  164
  - Removing the Exit Gas Cooler
    164
  - Removing the Sensors
    164
  - Removing Hoses, Filters and Pump Heads
    165
  - Removing Blanking Plugs
    166
  - Removing Threaded Adapters
    166
  - Removing the Inoculation Needle, Septum Collar and Septum
    166
  - Removing Addition Port Adapters
    167
  - Removing the Vessel Top Plate
    167
  - Removing the Pocket for Temperature Sensor (Pt100)
    169
  - Removing the Sparger and the Dip Tube(S)
    169
  - Removing the Baffles
    169
  - Removing the Impellers
    170
  - Removing the Stirrer Shaft
    170
- Cleaning and Storing Individual Parts
  171
- Cleaning the Sensors
  172
- Cleaning the Hoses and Pump Heads
  173
- Cleaning the Super Safe Sampler
  173
- Cleaning the Exit Gas Cooler
  174
- Cleaning the Basic Unit and Operating Panel
  174
- Cleaning the Heater Pad
  175
- Maintenance Plan
  175
- January
  175
- Lubricating the Mechanical Seal
  177
- Decalcifying the Equipment
  177
- Decalcifying Equipment Via Vessel Jacket
  179
11 Interferences

180
- Interferences Basic Unit and Operating Panel
  180
- Interferences Drive System
  181
- Interferences Temperature Control System
  181
- Interferences Gassing System
  183
- Interferences Ph-System
  184
- Interferences Po
  185
  - System
    185
- Interferences Antifoam or Level Sensor and Antifoam Pump
  186
- Interferences Feed and Pump
  187
- Returning for Repair
  188
12 Disassembly and Disposal

189
- Disassembly
  189
- Disposal
  189
13 Technical Data

191
- Dimensions 1 Unit
  191
- Dimensions Master Unit and Satellite Units
  193
- Dimensions of Culture Vessels in Vessel Holder
  194
- Weights (Net)
  196
- Connection Values
  196
  - Electrical
    196
  - Water in
    196
- Specifications
  197
  - Water out
    197
  - Gas(Es) in
    197
  - Exit Gas
    197
  - Operating Unit
    197
  - Culture Vessel
    198
  - Stirrer
    198
  - Temperature
    200
  - Gassing
    201
  - Antifoam
    202
  - Pumps
    204
  - Sterile Filters
    205
    
    Heater Pads for Single Walled Vessels
    206
    
    Auxiliary Supplies
    207

Infors HT Labfors 5 User Instructions (32 pages)

Single-Use Vessel Option

Brand: Infors HT | Category: Laboratory Equipment | Size: 0 MB

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Infors HT Labfors 5 Manuals

Infors HT Labfors 5 Operating Manual (208 pages)

Table of Contents

1 General Information

2 Safety and Responsibility

3 Setup and Function

4 Options

5 Accessories

6 Transport and Storage

7 Installation and Initial Operation

8 Before Fermentation/Cultivation

9 Fermentation/Cultivation

10 Cleaning and Maintenance

11 Interferences

12 Disassembly and Disposal

13 Technical Data

Infors HT Labfors 5 User Instructions (32 pages)

Table of Contents

1 General Information

2 Safety

3 Technical Data

4 Setup and Function

5 Procedures to Carry out Prior to Cultivation

6 Cultivation

7 Procedures to Carry out after Cultivation

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