Infors HT Multifors 2 Operating Manual page 145

28 March 2018
CAUTION!
Risk of damage to the pH sensor!
The glass membrane of the pH sensor can be
damaged due to drying out. The water vapour gen-
erated by sterilisation is sufficient for moistening it.
Therefore:
– Never sterilise a completely empty and dry ves-
sel.
Fill the vessel with a little water, approx. 10 mL per litre of

working volume to generate sufficient steam in the vessel dur-
ing sterilisation. Add basal salts if needed.
Sterilise the vessel.

Add the culture medium and inoculum under sterile conditions.

All heat-labile components are usually sterile filtrated and
added by injection or with the inoculum afterwards.
NOTICE!
This method is often used for cell cultures.
Multifors 2 - Parallel Bench-top Bioreactor
Preparations for Use
Page 145 of 211