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Summary of Contents for Infors HT Labfors 5 Cell
- Page 1 User Instructions 2014-05-27 Labfors 5 Cell Single-Use Vessel Option Dok-ID: V.01.000 - Original...
- Page 2 Infors AG Infors USA Inc. Headoffice, Schweiz P.O. Box 1380 Rittergasse 27 Laurel, MD 20725 CH-4103 Bottmingen T +1 301 362 3710 / 855 520 7277 (toll-free USA) T +41 (0)61 425 77 00 F +1 301 362 3570 F +41 (0)61 425 77 01 infors.usa@infors-ht.com headoffice@infors-ht.com www.infors-ht.us...
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Page 3: Table Of Contents
Labfors 5 Cell - Single-Use Vessel Option Table of Contents General Information .............5 About this Document ...........5 About the Device Option ..........5 Safety ...................6 Personnel ..............6 Personal Protective Equipment ........7 Technical Data ..............8 Stirrer Drive ..............8 Temperature ..............8 Setup and Function ............10 Overview Labfors 5 Cell with Single-Use Vessel Option ............... - Page 4 Labfors 5 Cell Single-Use Vessel Option Inhaltsverzeichnis Sterilising Sensors and Reagent Bottles following Cultivation ..............30 Cleaning ..............31 Page 4 of 31 27 May 2014...
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Page 5: General Information
1.1 About this Document This document and the operating manual of the benchtop bioreactor Labfors 5 are component part of the Labfors 5 Cell with option for single-use vessels. Both documents must be kept near to the equipment and must be accessible to the operators at all times. -
Page 6: Safety
Labfors 5 Cell - Single-Use Vessel Option Safety 2 Safety Non-observance of the operational descriptions and safety instructions listed in the operating manual of the bioreactor Labfors 5 and in this manual may lead to serious hazards. Inappropriately carried out work... -
Page 7: Personal Protective Equipment
Labfors 5 Cell - Single-Use Vessel Option Safety 2.2 Personal Protective Equipment It is essential to wear personal protective equipment to minimise health hazards Always wear the personal protective equipment which the particular activity requires. Always follow instructions available in the workplace,... -
Page 8: Technical Data
Labfors 5 Cell - Single-Use Vessel Option Technical Data 3 Technical Data 3.1 Stirrer Drive Description Value Motor DC Motor, brushless Motor with direct coupling to the drive Drive shaft in the single-use vessel Nominal power 100 W Nominal torque... - Page 9 Technical Data For details about technical data concerning the bioreactor Labfors 5 Cell in general refer to the main chapter “Technical Data” in the operating manual of the bioreactor Labfors 5. For details about technical data concerning the single-use vessel refer to the documentation of the manufacturer Merck Millipore™.
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Page 10: Setup And Function
Labfors 5 Cell - Single-Use Vessel Option Setup and Function 4 Setup and Function 4.1 Overview Labfors 5 Cell with Single-Use Vessel Option Touchscreen operating panel Single-use vessel Motor with adaptor Heater pad Basic unit Page 10 of 31 27 May 2014... -
Page 11: Single-Use Vessel
Temperature measurement is made by the temperature sensor (Pt-100) integrated in the basic unit of the Labfors 5 Cell. It is simply inserted into the thermowell in the single-use vessel. -
Page 12: Stirrer Drive
Labfors 5 Cell - Single-Use Vessel Option Setup and Function 4.1.3 Stirrer Drive Motor Coupling adaptor Adaptor sleeve The vessel stirrer shaft is driven by the brushless DC standard motor of Labfors 5 Cell. The magnetic coupling adaptor must be fitted onto the motor and the adaptor sleeve put on the drive hub on the top plate of the single-use vessel. -
Page 13: Connection With Gassing And Fluid Addition
All major parameters, such as e.g. temperature, gassing, stirrer speed and addition of further media is controlled via the operating and control panel of the Labfors 5 Cell. Sterilisable standard pH and pO sensors are used for pH and pO control. -
Page 14: Procedures To Carry Out Prior To Cultivation
5 Procedures to carry out prior to Cultivation This chapter describes the procedures before fermentation relating to equipment supplied as part of the Labfors 5 Cell system. This includes the following: Preparing the reagent bottles and pump heads and sterilising... -
Page 15: Preparing The Reagent Bottles And Hoses
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation 5.1 Preparing the Reagent Bottles and Hoses Reagent bottles with two connections are available as standard. Bottle sizes from 250 mL up to 5000 mL are available. They are equipped ex-factory. - Page 16 Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation Fit a hose connector on each pump hose on the pump head and secure the hoses with cable ties. Fig. 1 Cut a longer piece of silicone hose.
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Page 17: Equipping A Reagent Bottle For Connection With A Fluid Inlet Line
5.1.2 Equipping a Reagent Bottle for Connection with a Fluid Inlet Line Accessories for connection of the single-use vessel with the Labfors 5 Cell are purchased in the starter kit. For later connection of the reagent bottle with a fluid inlet (“Fluid Addition Line”) on the single-use vessel equip the silicone hose as... -
Page 18: Calibrating The Ph Sensor
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation 5.2 Calibrating the pH Sensor The pH sensor must be calibrated before sterilisation and subsequent mounting into the single-use vessel. For details about the calibration refer to the chapter “Calibrating the pH sensor”... - Page 19 Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation Check and ensure the following before beginning: The pH sensor is calibrated The cable connector of the pH sensor is covered with its red protective cap The pH sensor is placed in a sterilisable container filled with sufficient water to ensure the tip of the sensor is immersed.
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Page 20: Equipping The Gassing Line On The Equipment Side
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation 5.4 Equipping the Gassing Line on the Equipment Side The gassing line on the equipment side (silicone tube fitted onto metal olive below rotameter) must be equipped with a male Luer Lock connector for later connection with the single-use vessel. -
Page 21: Fitting And Connecting The Heater Pad
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation The temperature sensor (Pt-100) must be inserted into the thermowell in the single-use vessel. Adhere to the instructions in the documentation from the manufacturer Merck Millipore™. - Page 22 (“Fluid Addition Lines”) on the single-use vessel. Hook up the tensioning springs. Plug the connector of the heater pad into the socket (“Heating mat”) on the basic unit of the Labfors 5 Cell. Page 22 of 31 27 May 2014...
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Page 23: Connecting The Motor
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation 5.8 Connecting the Motor To connect the motor with the single-use vessel and the base unit proceed as follows: Procedure Fit and adjust the adaptor sleeve onto the drive hub on the single-use vessel. -
Page 24: Connecting The Gassing
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation Plug the connector of the motor cable into the socket on the motor. The cable connector must be correctly located onto the socket on the motor; it will only plug in when orientated properly. -
Page 25: Calibrating The Po Sensor
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out prior to Cultivation 5.10 Calibrating the pO Sensor When the single-use vessel is fully connected with the basic unit of Labfors 5 Cell and the pO sensor (polarographic type) is polarised, the pO sensor must be calibrated. -
Page 26: Cultivation
Labfors 5 Cell - Single-Use Vessel Option Cultivation 6 Cultivation 6.1 Safety CAUTION! Risk of burns due to hot surfaces and danger of loss of property due to overheating! Cultivation with the temperature sensor (Pt-100) not inserted or with an insufficiently filled single-use vessel carries a risk of overheating the vessel. -
Page 27: Procedures To Carry Out After Cultivation
Disconnecting all cable and hose connections between the basic unit of Labfors 5 Cell and the single-use vessel: Disconnecting the silicone hoses from the fluid inlets (“Fluid Addition Lines”) on the single-use vessel... -
Page 28: Disconnecting Cable And Hose Connections, Removing The Sensors
They are not described in this document. 7.2 Disconnecting Cable and Hose Connections, Removing the Sensors To disconnect all cable and hose connections between the basic unit of Labfors 5 Cell and the single-use vessel, proceed as follows: Motor and heater pad... - Page 29 Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out after Cultivation Reagent bottles and gassing line When the fluid inlet lines (“Fluid Addition Lines”) are completely emptied: Procedure Clamp off the silicone hose near to the lid on the reagent bottle.
- Page 30 7.3 Sterilising Sensors and Reagent Bottles following Cultivation When all cable and hose connections between the basic unit of the Labfors 5 Cell and the single-use vessel have been disconnected and sensors have been removed: The sensors, reagent bottles and pump heads must be re-sterilised separately in an autoclave.
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Page 31: Cleaning
Labfors 5 Cell - Single-Use Vessel Option Procedures to carry out after Cultivation 7.4 Cleaning After sterilisation in the autoclave and sufficient time to cool down: Reagent bottles, pump heads, and sensors must be cleaned. If necessary, the heater pad can also be wiped clean.
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