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Infors HT Multitron Operating Manual

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Operating manual
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  • Page 1 Multitron Operating manual We bring life to your laboratory.
  • Page 2 Multitron – Rel. 4.1 Incubator shaker FW: 3.2 Doc-ID: D006, 3, en_GB – Original Art. 7000682 More information about the product is INFORS HT available online at: Headoffice, Switzerland www.infors-ht.com/en/multitron Rittergasse 27 CH-4103 Bottmingen T +41 (0)61 425 77 00 info@infors-ht.com...
  • Page 3 Multitron - Operating manual Supplemental directives Supplemental directives About this Manual This manual enables the safe and efficient handling of the device. All the information and instructions in this operating manual comply with the current standards, legal regulations and the latest technological developments.
  • Page 4 2.4 Qualified Personnel........................21 2.4.1 Operator..........................21 2.4.2 Technician......................... 22 2.4.3 INFORS HT Service Technician or Licensed Dealer..22 2.5 Unauthorised Persons......................22 2.6 Responsibility of the Provider..................22 2.7 Residual Risks............................ 23 2.8 Safety Features..........................25 2.9 Warning Symbols on the Device................26 2.10 Declaration of Decontamination................
  • Page 5 Multitron - Operating manual Table of contents 3.2.3 Ethernet Interface....................32 3.2.4 USB Connection......................32 Openings..............................33 3.3.1 Discharge Outlet...................... 33 3.3.2 Air Vents..........................33 3.3.3 Ventilation Opening..................... 34 3.4 Working Light..........................34 3.5 Substructure............................34 3.6 Operating and Display Elements................35 3.6.1...
  • Page 6 Multitron - Operating manual Table of contents 4.6 Darkening.............................. 47 4.7 Analogue Output......................... 48 4.7.1 Setup and Function....................48 4.7.2 Connection Assignment................. 48 4.8 Cable Pass-through........................49 4.8.1 Setup and Function....................49 4.8.2 Using the Cable Pass-through..............50 4.9 Antimicrobial Coating......................51 5 Accessories..............................
  • Page 7 Multitron - Operating manual Table of contents 7.2.3 Mounting the Holders..................76 7.2.4 Tips & Tricks for Loading the Tray............79 Setting, Activating and Deactivating Parameters......80 7.4 Timer Function..........................81 7.4.1 Overview..........................81 7.4.2 Programming Timer....................83 7.4.3 Starting the Timer....................85 7.4.4 Viewing and Changing Timer Settings.........
  • Page 8 Multitron - Operating manual Table of contents 10 Transport and Storage........................112 10.1 Transport..............................112 10.2 Storage..............................112 11 Disassembly and Disposal......................113 11.1 Disassembly............................113 11.2 Disposal..............................113 12 Technical Data............................. 115 12.1 Dimension Drawings....................... 115 12.2 Connections and Interfaces................... 117 12.3 Specifications of the Basic Unit.................
  • Page 9 Multitron - Operating manual Overview of the Device Overview of the Device Kopiervorlagen diverse Basic Unit Kopiervorlagen diverse Kopiervorlagen diverse Exterior Kopiervorlagen diverse lagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen rlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse...
  • Page 10 Slide rail Discharge outlet Short Description The Multitron incubator shaker is used to cultivate microorganisms or cell cultures in a laboratory environment. The basic version of the device is fitted with a shaker drive and a heater. Depending on which version of the device you have bought, it will come with either a 3 mm, 25 mm or 50 mm shaking throw.
  • Page 11 Multitron - Operating manual Overview of the Device Stacking Devices If necessary, up to three devices can be stacked to save space. Devices can also be stacked subsequently but stacking must be per- formed by the manufacturer’s qualified personnel or persons author- ised by the manufacturer.
  • Page 12 Multitron - Operating manual Overview of the Device Parameters Parameter Display Unit Setting range Option Additional information Temp. Temperature °C 4.0 to 65.0 The temperature that can actually be achieved depends on a variety of factors (e.g. the ambient temperature around the...
  • Page 13 Multitron - Operating manual Overview of the Device Kopiervorlagen diverse Operating and Display Elements Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Status display (PIN function, remote control of the...
  • Page 14 Multitron - Operating manual Overview of the Device 1.3.1 Operating Elements Overview The device is controlled via keys in the operating section. The keys are colour-coded based on their function: Black: Setting keys ■ Blue: Timer selection key ■ Orange: Parameters and settings selection key ■...
  • Page 15 Multitron - Operating manual Overview of the Device Function Operating ele- Designation Function ment Select The Select key is used to select the parameters (also within the Timer function). The key is also used to exit the timer overview and the settings menu.
  • Page 16 Multitron - Operating manual Overview of the Device 1.3.2 Display Elements Parameter The following parameters are displayed in the upper view box: Temp. ) Temperature ( ■ concentration ( , optional) ■ The following parameters are displayed in the lower view box:...
  • Page 17 Multitron - Operating manual Overview of the Device Status Displays Symbol Meaning The control panel is locked with a PIN. If you try to make a setting, a PIN prompt appears. Once you enter the correct PIN, the symbol disappears.
  • Page 18 Multitron - Operating manual Safety and Responsibility Safety and Responsibility This chapter contains general information on safety when using the device. In the remaining chapters, warning messages are used only to highlight particular hazards directly arising from the actions being described.
  • Page 19 Multitron - Operating manual Safety and Responsibility Intended Use, Incorrect Use and Misuse Intended Use The device is designed to be used as an incubator shaker for cul- tivating microorganisms or cell cultures under the following condi- tions: Cultivation of non-pathogenic microorganisms or cell cultures ■...
  • Page 20 Multitron - Operating manual Safety and Responsibility The device is not protected against explosions. Use and manufac- ■ ture of explosive gases as well as operating the device in the Ex area are therefore not permitted. The device is not designed to sufficiently protect the personnel if ■...
  • Page 21 Multitron - Operating manual Safety and Responsibility Cultivating Organisms of Risk Cate- When cultivating pathogenic organisms of risk category 2, special gory 2 measures must be taken to stop the organisms from escaping. The user is responsible for this. When using the device under protection category 2, stainless steel clamps of the correct size must be used to affix the flasks.
  • Page 22 Disassembly, disposal and recycling specialists ■ 2.4.3 INFORS HT Service Technician or Licensed Dealer Certain work may only be performed by the manufacturer's skilled per- sonnel or by skilled personnel authorised by a licensed dealer. Other persons are not authorised to perform this work.
  • Page 23 Multitron - Operating manual Safety and Responsibility Provider Obligations The device is used for industrial and scientific purposes. As such, the provider of the device is individually liable with regard to the legal requirements relating to occupational health and safety in a biotech- nology laboratory.
  • Page 24 Multitron - Operating manual Safety and Responsibility Moving Parts Moving parts are a general hazard posed by the device because body parts can be pinched or scratched when one is not careful. However there is no risk of clothing or body parts being pulled into the device.
  • Page 25 Multitron - Operating manual Safety and Responsibility Pathogenic Organisms The device is not approved for cultivation of pathogenic organisms of risk categories 3 and 4. In the context of intended use, it is nonetheless possible for pathogenic organisms and viruses to be cultivated. Con- tact with pathogenic organisms bears a significant health risk.
  • Page 26 Multitron - Operating manual Safety and Responsibility Warning Symbols on the Device The following warning symbols (stickers) are placed on the device: Warning symbol Position Meaning On the housing of the Observe the instructions in the operating optional humidity con-...
  • Page 27 Multitron - Operating manual Safety and Responsibility 2.10 Declaration of Decontamination When returning the device for repair, disassembly or disposal, a legally compliant declaration of decontamination is required for the safety of all involved and due to legal requirements. The following must be...
  • Page 28 The shaking throw may only be adjusted by qualified personnel that has received appropriate training and authorisations. If in doubt, contact INFORS HT. iverse Shaking Table The shaking table (1) is used to hold the 85 x 47 cm (type M) tray, var- ious versions of which are available.
  • Page 29 Multitron - Operating manual Setup and Function Kopiervorlagen diverse Kopiervorlagen diverse n diverse Kopiervorlagen diverse Tray Lock The way the tray is fixed on the shaking table differs depending on the shaking throw. 25 and 50 mm throw 3 mm throw...
  • Page 30 Multitron - Operating manual Setup and Function 3.1.2 Temperature Control (Heating) Function Kopiervorlagen diverse Finned heater and axial fan (in back wall, not visible) Pt100 temperature sensor Kopiervorlage Bilderrahmen The temperature in the incubation chamber is regulated using a Pt100 temperature sensor with a PID controller.
  • Page 31 Multitron - Operating manual Setup and Function Connections and Interfaces 3.2.1 Mains Connection The mains connection is located on the right-hand side of the device. Three different versions of the device are available for different mains voltages: 230 V 50 Hz ■...
  • Page 32 For this purpose, a driver must be developed which translates the protocol used for the external software. The necessary documentation for the communi- cation protocol can be ordered from INFORS HT. The following data and states are transmitted via the Ethernet inter- face: From the device to the SCADA/bioprocess software: setpoints and ■...
  • Page 33 Multitron - Operating manual Setup and Function Openings 3.3.1 Discharge Outlet For draining spilled liquids, cleaning agents or accumulated condensa- tion water, there is a discharge outlet at the bottom, almost in the middle of the left side of the casing.
  • Page 34 Multitron - Operating manual Setup and Function 3.3.3 Ventilation Opening An opening for ventilation of the interior is located in the top centre of the rear of the housing. The purpose of ventilation is to supply bacterial cultures with oxygen from the air.
  • Page 35 Multitron - Operating manual Setup and Function Low Base Single units and three units stacked can be fitted with a low base. The base has an adjustable foot that can be used to level the device. High Base Single units and two units stacked can be fitted with a high base. This has an adjustable foot that can be used to level the device.
  • Page 36 Multitron - Operating manual Setup and Function 3.6.2 Operating Panel All device functions can be controlled directly via the operating panel on the front of the device. The operating panel is divided into a display section and an operating section.
  • Page 37 Multitron - Operating manual Setup and Function 3.7.2 Identification of the Throw On the back wall inside the incubation chamber, there is a sticker that Throw ). identifies the throw ( 27.05.2024 Page 37 of 140...
  • Page 38 Multitron - Operating manual Options Options To adapt the device to the specific usage scenarios, the device can be enhanced with various options. The options can be ordered at the same time as the device or can be fitted at a later stage. For detailed information on the options as well as advice, please contact our customer service.
  • Page 39 Multitron - Operating manual Options The cooling unit can either be placed in a bracket on the incubator shaker or in the high base (only the variant with 900 W). The variant with 1800 W is used when very low temperatures are to be reached.
  • Page 40 Multitron - Operating manual Options Connection Conditions Cooling medium inlet Cooling medium outlet The connections for an external cooling system are on the top of COOLANT IN (inlet) and the device. The connections are labelled COOLANT OUT (outlet). If several devices with external cooling are stacked, the lines are connected internally and only the top unit has connections on the top.
  • Page 41 Multitron - Operating manual Options n diverse Hygienic Humidity Control (HHC) 4.2.1 Setup and Function Kopiervorlagen diverse Bilderrahmen Kopiervorlage Bilderrahmen Hygienic Humidity Control Humidity sensor Part of the medium can evaporate, in particular in case of small vol- umes of work (e.g. when using microtitre and deep well plates) and long cultivation processes.
  • Page 42 Wasser Ein Multitron - Operating manual Options Air In Incubation chamber Air pump Steam / Air Out Evaporator chamber Water pump Heating Water In For pumping water and air, as well as for generating steam, the device is equipped with a water pump, air pump and an evaporation chamber.
  • Page 43 Multitron - Operating manual Options 4.2.2 Connection Conditions Before commissioning the humidity control, the unit must be con- nected to the water supply. There are two types of water supply: Unpressurised from a water tank ■ With inlet pressure of max. 2 bar from a pressurised water pipeline ■...
  • Page 44 Multitron - Operating manual Options HEAT state, check If the parameter is constantly in the if the “Temperature” parameter is activated. This must be switched on in order to use humidity control. For information on how to set and activate the parameter, see ⮫ Chapter 7.3 ‘Setting, Activating and Deactivating Parameters’...
  • Page 45 Multitron - Operating manual Options 4.3.2 Setup and Function Functional Description The optional CO control makes it possible to enrich the atmosphere in the interior by 0 to 20 % CO . CO control is particularly suited to culti- vation of mammalian cells and algae because the CO ensures that the pH value in the culture medium is stable.
  • Page 46 Multitron - Operating manual Options Hoses The connection hose must satisfy the following conditions: Use only pressure-resistant and intact hoses. ■ The hose must have an appropriate diameter; an adapter may be ■ used, if necessary. Secure hoses using appropriate hose clamps.
  • Page 47 Multitron - Operating manual Options Removable Shelf A shelf (approx. 865 mm x 490 mm) for static incubation is available as an option. This facilitates static incubation in climatic conditions almost identical to the cultures on the shaking table. NOTICE...
  • Page 48 Multitron - Operating manual Options Analogue Output 4.7.1 Setup and Function The device can optionally be equipped with an analogue output module. This provides 8 channels via which actual values and setpoints can be transferred to an external device. The analogue output module is located on the rear and is in its own housing.
  • Page 49 Multitron - Operating manual Options Cable Pass-through 4.8.1 Setup and Function A pass-through for cables and hoses can be installed on the right side of the housing. This can be used to guide additional sensors or gassing into the incubation chamber.
  • Page 50 Multitron - Operating manual Options 4.8.2 Using the Cable Pass-through WARNING If an external device that is supplied with mains voltage is operated on the shaking table whilst it is operating, there is a risk that the cable might break or get jammed. This poses a risk of death by electrocution.
  • Page 51 Multitron - Operating manual Options Antimicrobial Coating The housing of the device is optionally available with an antimicrobial coating. This contains a coating additive of pure metallic silver that reliably kills microorganisms that come into contact with the surface. This can inhibit the growth of bacteria, fungi and other microorgan- isms on the walls in the incubation chamber.
  • Page 52 Multitron - Operating manual Accessories Accessories The following chapters describe all accessories that are generally available for the device. For special applications, please contact our customer service for advice. Trays Different trays are available for the device; these can be purchased individually or ordered with the device.
  • Page 53 Multitron - Operating manual Accessories Figure Designation Tray with pin holders for microtitre and deep-well plates Tray with clamping assembly for microtitre and deep-well plates Tray with sliding bars Tray with Sticky Stuff 27.05.2024 Page 53 of 140...
  • Page 54 Multitron - Operating manual Accessories 5.1.1 Universal Table Tray Overview The tray referred to as “universal table tray” features drill holes on a grid so that it can be equipped as desired. For equipping, various clamps and test tube holders are available; these can be combined as desired (⮫ Chapter 5.2 ‘Clamps and Other Holders’...
  • Page 55 Multitron - Operating manual Accessories 5.1.2 Tray with Steel Clamps Fitting identical clamps on the entire tray results in the following capacities: Flask size [mL] Number of flasks per M tray 1000 2000 3000 4000 5000 5.1.3 Tray with Pin Holders...
  • Page 56 Multitron - Operating manual Accessories Quickload spacers are long plastic strips ervorlagen diverse (4 mm high) that span across several plates, thus allowing you to save time while loading. NOTICE The spacers are made of plastic and therefore cannot be autoclaved.
  • Page 57 Multitron - Operating manual Accessories Kopiervorlagen diverse verse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlage Bilderrahmen Kopiervorlage Bilderrahmen Kopiervorlagen diverse Tray with clamping assemblies tilted at an angle of 20° (angled incu- Kopiervorlage Bilderrahmen bation): The folded clamping assembly is wedge-shaped metal sheet erse with two profiles.
  • Page 58 Multitron - Operating manual Accessories Technical Data Data Value Rail material Aluminium Foam rubber material CR rubber Temperature range ≤ 80°C Cleaning Mild neutral cleaning agent Disinfection Commercially available disinfectant Sterilisation in an autoclave Size of microtitre plates/deep- 85.1 x 127 mm well plates 5.1.5...
  • Page 59 Multitron - Operating manual Accessories 5.1.6 Tray with Sticky Stuff NOTICE If condensate forms on the tray or the culture vessels, the adhesive- ness of the Sticky Stuff adhesive matting is no longer guaranteed. This can result in cultivation vessels separating from the adhesive matting and breaking.
  • Page 60 Multitron - Operating manual Accessories Prior to the shaking process, gently pull on every vessel to ensure ■ they are all stuck on firmly. If the humidity is too high or the temperatures are too low or if ■ there are extreme jumps in temperature (e.g. when using the timer function), keep an eye on condensate.
  • Page 61 Multitron - Operating manual Accessories Service Life Sticky Stuff is a consumable that typically has to be replaced every 2 to 5 years. The service life depends on how the material is used and cleaned. If you are regularly using aggressive chemicals to clean or disinfect, we recommend replacing the Sticky Stuff every 2 years.
  • Page 62 Multitron - Operating manual Accessories Maximum Rotation Speeds with To ensure that the flasks do not detach from the adhesive matting, the Sticky Stuff maximum permissible rotation speed is limited when using adhesive matting. The following guidelines only apply to undamaged, completely dry adhesive matting and flasks free of grease.
  • Page 63 Multitron - Operating manual Accessories Stainless Steel Clamps The following stainless steel clamps are available: For flask type Volume Erlenmeyer 25 mL 500 mL 4000 mL 50 mL 1000 mL 5000 mL 100 mL 2000 mL 250 mL 3000 mL...
  • Page 64 Multitron - Operating manual Accessories 5.2.2 Test Tube Holder Test tube holders are used to securely affix different sized test tubes. Test tube holders can be screwed onto a universal table tray or placed on Sticky Stuff adhesive matting. The following test tube holders are available (details and special var- iants on request): For long test tubes;...
  • Page 65 Multitron - Operating manual ervorlagen diverse Accessories Setup Filter Filter washer Top plate Microtitre insert Quickload spacers en diverse orlagen diverse Container iervorlage Bilderrahmen e Bilderrahmen orlage Bilderrahmen Installation The box for microtitre plates is affixed to the tray with four Philips head screws.
  • Page 66 Multitron - Operating manual Accessories Sterilising The box for microtitre plates can be autoclaved (recommendation: 20 min at 120 °C). To do so, remove the Quickload Spacers first and disinfect them using commercial disinfectant. Limiting the Rotation Speed When there are high rotation speeds or insufficient grip due to humidity or dirt, the box for microtitre plates can separate from the Sticky Stuff and cause damage to the incubation chamber.
  • Page 67 Multitron - Operating manual Accessories Loading Capacity Data Value Microtitre plates 18 units Deep-well plates, height 42 mm 12 units Valid for standard plate size 85.1 x 127 mm. Profibus DP and Modbus TCP Gateways The Profibus DP gateway and the Modbus TCP gateway are used to connect the incubator shaker to a PLC.
  • Page 68 Multitron - Operating manual Accessories Profibus DP Gateway Modbus TCP Gateway PLC / SCADA PLC / SCADA PLC / SCADA Network switch Network switch Network switch Page 68 of 140 27.05.2024...
  • Page 69 Thanks to soft-sensors, additional knowl- edge can be generated. In addition to INFORS HT products, biotech machines and analysis devices from third-part manufacturers can be connected. This makes it possible to holistically control, monitor and analyse bioprocesses using a software.
  • Page 70 Multitron - Operating manual Installation and Commissioning Installation and Commissioning Only the manufacturer’s qualified expert personnel or persons author- ised by the manufacturer may install and initially commission the device. Hence, the following section only lists the requirements for the installation location to be observed by the provider.
  • Page 71 Multitron - Operating manual Installation and Commissioning Unsuitable Installation Sites In order to achieve the desired climate in the incubation chamber and to be able to control it as precisely as possible, the following installation locations must be avoided at all costs: Poorly ventilated recesses or areas in the exhaust heat air stream ■...
  • Page 72 Multitron - Operating manual Installation and Commissioning Minimum Distances to the Device When setting up the device, the following minimum distances must be observed to ensure adequate ventilation and access to the most important connections: Minimum Distances to the Basic Device...
  • Page 73 Multitron - Operating manual Installation and Commissioning Placing the Device under a Table When placing the device under a table, observe the following: You must leave a ventilation gap of at least 100 mm between the ■ device and rear wall of the table to ensure heat produced by the device can escape.
  • Page 74 Multitron - Operating manual Operation Operation Switching the Device on NOTICE The shaker drive can start up automatically, if the device was not switched off correctly beforehand. Loose items in the incubation chamber can damage the device and cultivation vessels if this hap- pens.
  • Page 75 Multitron - Operating manual Operation If you wish to stop the shaker drive slowly, e.g. to avoid culture liquid from spilling out, you have to OFF prior to set the “Rotation speed” parameter to opening the door. In this case, the shaker drive stops slowly, with the brake force set in the Settings menu (⮫ ‘Set Brake Power’...
  • Page 76 Check the incubation chamber for foreign objects and remove these. − If it is still not possible to insert the tray correctly, contact your nearest INFORS HT representative. 7.2.3 Mounting the Holders NOTICE The threaded holes of the tray can be damaged if the fastening screws are not screwed straight into the thread.
  • Page 77 Multitron - Operating manual Operation NOTICE All holders are supplied with pre-fitted screws. If a test tube holder or box for microtitre plates is placed on the Sticky Stuff adhesive matting, there is a risk that the adhesive matting is damaged by protruding screws.
  • Page 78 Multitron - Operating manual Operation Screw Sizes NOTICE If screws that are too long are used to install the holders, these will stick out at the bottom of the tray. This has the effect that the tray can no longer be inserted and affixed correctly.
  • Page 79 Multitron - Operating manual Operation 7.2.4 Tips & Tricks for Loading the Tray Observe the following notices when loading the tray: The working volume should not exceed 1/3 of the total volume of ■ the vessel. To ensure smooth operation, place the cultivation flasks symmetri- ■...
  • Page 80 Multitron - Operating manual Operation Setting, Activating and Deactivating Parameters Setting the Setpoint and Switching To set the setpoint for a parameter and switch on the parameter, on the Parameter proceed as follows: Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Kopiervorlagen diverse Use the Scroll key (1) to select the desired parameter pair.
  • Page 81 Multitron - Operating manual Operation When starting up the shaker drive, use the time until the setpoint is reached to check whether the cultiva- tion flasks are secured on the tray. The parameters need a little time to reach their set setpoints.
  • Page 82 Multitron - Operating manual Operation More complex sequences or processes can be pro- grammed using the eve bioprocess platform soft- ® ware. If the device is controlled remotely via the Ethernet interface (e.g via eve ), the timer function is blocked.
  • Page 83 Multitron - Operating manual Operation To program a one-time change from phase 1 to phase 2, the timer must be deactivated for the second Time 2 = OFF ). If the timer is activated for phase ( Time 2 = ON ), the device switches the second phase ( cyclically between phases (see next example).
  • Page 84 Multitron - Operating manual Operation Set the parameters for phase 1: Use the Select key to select the desired parameter. ■ Activate the parameter with the lower ON/OFF key. ■ Use the lower Plus or Minus key to set the setpoint.
  • Page 85 Multitron - Operating manual Operation 7.4.3 Starting the Timer To start a programmed timer, proceed as follows: In the Timer overview, press either the upper or the lower ON/OFF key. �� As soon as the timer function has been activated, the two phases incl.
  • Page 86 Multitron - Operating manual Operation 7.4.4 Viewing and Changing Timer Settings The settings (duration of the phases as well as parameter setpoints) of a running timer can be viewed and adjusted if necessary. Depending on whether the setpoints or the duration of the phases are changed,...
  • Page 87 Multitron - Operating manual Operation 7.4.5 Stopping the Timer The timer can be stopped at any time. To do so, proceed as follows: Press the TIMER key to select the timer function. Press the ON/OFF key on the upper or lower control panel to switch off the timer function.
  • Page 88 Multitron - Operating manual Operation Using eve to Operate the Device ® Overview For detailed information on how to use eve ® operate the device, please read the documentation supplied with eve ® The Ethernet interface of the device allows the device to be operated...
  • Page 89 Multitron - Operating manual Operation Displays on the Device If the device is controlled using eve , this is displayed on the device as ® follows: While the device is being registered in eve , the working light of ®...
  • Page 90 Multitron - Operating manual Operation Settings Menu (Option Function) 7.6.1 Opening the Settings Menu The Settings menu enables you to adjust the main device settings. To open the device’s Settings menu, proceed as follows: Press the OPTION key. �� The functions in the Settings menu are displayed as a list. The selected function is displayed in a large black font.
  • Page 91 Multitron - Operating manual Operation 7.6.3 Settings Event List The event list shows the last 18 events including their timestamps (date and time). The latest event is always at the top. The following events are displayed in the event list: Activating/deactivating parameters (manually on the device, ■...
  • Page 92 Multitron - Operating manual Operation Set PIN To prevent unauthorised persons making entries on the operating panel, the key pad can be locked using a PIN. To set a PIN, use the lower ON/OFF key to activate the key pad lock.
  • Page 93 Multitron - Operating manual Operation Set Time Setting the local time. This is necessary to ensure that the timestamp in the event list is displayed correctly. To set the time, use the lower SET key to select the part of the time (hour or minutes) that is to be changed.
  • Page 94 Multitron - Operating manual Operation Set Brake Power Setting the braking power with which the shaker drive is stopped when the “Rotation speed" parameter is switched off. At brake force level 0 the shaking table is stopped by reducing the ■...
  • Page 95 Multitron - Operating manual Operation Setting the Adjustable Throw CAUTION Improper modification of the shaking throw of the device with a variable shaking throw is a safety risk. The shaking throw may therefore only be moved by qualified personnel that has received appropriate training and authorisations.
  • Page 96 Multitron - Operating manual Operation Behaviour in Case of a Power Interruption If the power supply to the device is interrupted during a running cultivation process (e.g. by pressing the power switch or in case of a power failure), all parameters and timer setpoints as well as the residual time of the last active timer phase are stored.
  • Page 97 − Never remove the covers of the device. − Damaged parts may only be replaced by an INFORS HT service technician, a licensed dealer or authorised expert personnel. − Contact the manufacturer in case of faults that cannot be resolved by following the notices below.
  • Page 98 Multitron - Operating manual Rectifying Faults Alarm Meaning Deviation from Waiting time setpoint Temp HIGH / LOW Temperature too high/low > ± 1 °C 20 min (18 °C to 40 °C) 75 min (< 18 °C / > 40 °C)
  • Page 99 Error message Description Solution ERROR: TEMPERATURE SENSOR The measured temperature falls Contact your local INFORS HT rep- outside the permissible range. The resentative. temperature sensor is defective. ERROR: FAN 1-4 At least two of the four axial fans Contact your local INFORS HT rep- are blocked or defective.
  • Page 100 Rectifying Faults Error message Description Solution ERROR: DRIVE BELT BROKEN The drive belt is torn. Contact your local INFORS HT rep- resentative. ERROR: MOTOR CONTROL The motor or the controller of the Contact your local INFORS HT rep- motor is defective.
  • Page 101 ERROR: DISPLAY HW The display unit is defective. Contact your local INFORS HT rep- resentative. Fault Tables The tables below describe potential faults that do not usually trigger an error message on the display or – with a few exceptions – an alarm signal.
  • Page 102 Multitron - Operating manual Rectifying Faults General Faults Fault description Cause Remedy Personnel After activating the Power supply of the device Check if the plugs are Operator ■ power switch, the dis- interrupted. plugged in correctly. play and the power Check the mains con- ■...
  • Page 103 Multitron - Operating manual Rectifying Faults Faults in Conjunction with the “Rotation Speed” Parameter Fault description Cause Remedy Personnel Strong vibrations occur. The load is distributed unevenly. Load the tray in the Operator centre. If possible, do not place any heavy weights in the corners of the tray.
  • Page 104 Re-measuring the temperature only provides reliable information when using calibrated measuring tools, and only for measurements at points specified by INFORS HT. Measurements taken at undefined points in the housing will not produce any usable data. For information about temperature measuring, con- tact your local INFORS HT representative.
  • Page 105 Multitron - Operating manual Rectifying Faults Faults in Conjunction with the “CO Concentration” Parameter Fault description Cause Remedy Personnel concentration too cylinder is empty. Install new CO cylinder. Operator low (deviation > 1%). Inlet pressure or flow too low. Increase inlet pressure or Operator flow.
  • Page 106 Multitron - Operating manual Rectifying Faults Replacing Device Fuses The device fuses may only be replaced by fuses of the same rating. For information on the requirements regarding fuses, see ⮫ Chapter 12.3.3 ‘ Electrical Con- nection and Power Values’ on page 120.
  • Page 107 − Never remove the covers of the device. − Damaged parts may only be replaced by an INFORS HT service technician, a licensed dealer or authorised expert personnel. Maintenance The device requires hardly any maintenance. This reduces the running costs to certain regular checks and cleaning.
  • Page 108 Multitron - Operating manual Cleaning and Maintenance Interval Maintenance work Personnel Annually If you are using humidity and CO sensors, have these Technician calibrated at least once a year to ensure measuring results remain accurate. When using the optional Hygienic Humidity Control INFORS HT service techni- (HHC): Have maintenance performed annually.
  • Page 109 Multitron - Operating manual Cleaning and Maintenance Notices on Cleaning To clean the surfaces, use a soft cloth, ideally lint-free. This applies in particular to the front window. Disinfect using commercially available disinfectant if necessary. Spray When cleaning the base tray, only use a wet cloth, never pour water into the tray.
  • Page 110 Replacement screws are supplied together with the device. If you lose the spare screws or all replace- ment screws have been used, contact INFORS HT. To dismantle the shaking table and to clean the base tray, proceed as...
  • Page 111 Multitron - Operating manual Cleaning and Maintenance Hold the shaking table in its tilted up position (or, ideally, get a second person to do so), or use a suitable object to hold it there. Cleaning the Base Tray Clean the base tray with a mild cleaning agent. Larger quantities of liquid can be drained off via the discharge outlet on the left side.
  • Page 112 10 Transport and Storage Inbound delivery and transport to the assembly location are per- formed only by INFORS HT employees or by persons authorised by INFORS HT. Nonetheless it is possible that the provider’s personnel is entrusted with transport tasks in the context of on-site transport. In this case, observe the following notes.
  • Page 113 Multitron - Operating manual Disassembly and Disposal 11 Disassembly and Disposal The device must be disassembled and disposed of in an environmen- tally-friendly manner if it is not in use anymore. If the device is to be returned to the manufacturer for...
  • Page 114 Multitron - Operating manual Disassembly and Disposal For disposal, the system units must be disassembled and dismantled into individual material groups. These materials must be disposed of according to the applicable national and local legislation. Local author- ities or specialist disposal firms can provide information regarding environmentally acceptable disposal.
  • Page 115 Multitron - Operating manual Technical Data 12 Technical Data 12.1 Dimension Drawings Two units stacked with high base and the following options: Low cover (1) ■ Kopiervorlagen diverse control (2) ■ Kopiervorlagen diverse Analogue output (3) ■ Hygienic Humidity Control (HHC) (4) ■...
  • Page 116 Multitron - Operating manual Technical Data Three units stacked with low base and the following options: Top cooling (1) ■ rlagen diverse control (2) ■ Analogue output (3) ■ Hygienic Humidity Control (HHC) (4) Kopiervorlagen diverse ■ Note: The indicated values apply to the 230 V version. The base of the 115 V version is slightly higher (140 mm instead of 130 mm).
  • Page 117 Multitron - Operating manual Technical Data verse Kopiervorlagen diverse Kopiervorlagen diverse 12.2 Connections and Interfaces Kopiervorlagen diverse Two Units Stacked with a High Base Note: The indicated values apply to the 230 V version. The base of the 115 V version is slightly lower (406 mm instead of 450 mm).
  • Page 118 Multitron - Operating manual Technical Data Three Units Stacked with a Low Base Note: The indicated values apply to the 230 V version. The base of the 115 V version is slightly higher (140 mm instead of 130 mm). E lectrical pow er supply: 230 V , 7.2 A, 50 - 60 H z...
  • Page 119 Multitron - Operating manual Technical Data 12.3 Specifications of the Basic Unit 12.3.1 Weight Data Value Unit Single unit (3 mm throw) Single unit (25 mm throw) Single unit (50 mm throw) Single unit (adjustable throw) Low base (230 V)
  • Page 120 Multitron - Operating manual Technical Data 12.3.3 Electrical Connection and Power Values Type 230 V Data Value Unit Voltage Frequency 50/60 Max. power consumption 1650 Max. current consumption Device fuses 10 A (two 5 x 20 mm fuses, time lag) Overvoltage category II  ...
  • Page 121 Multitron - Operating manual Technical Data Ethernet Interface Data Value Type RJ45 Data transfer 10/100 Mbps Ethernet Discharge Outlet Data Value Unit Hose nozzle 1/4 Inch Hose diameter 10 mm 12.3.5 Working Light Data Value Unit Power consumption 350 mA Power 12.3.6...
  • Page 122 Multitron - Operating manual Technical Data 12.3.8 Operating Conditions Data Value Unit Temperature range 10 to 30 °C Relative humidity, non-con- 10 to 85 densing Altitude operating location max. 2000 M above sea level Pollution degree as per EN 61010-1 Maximum load Min.
  • Page 123 Multitron - Operating manual Technical Data Specification Approved/applied products Refrigerant (primary cooling cir- R134a cuit, cooling compressor) Cooling liquid (secondary Based on 1,2-propanediol ■ cooling circuit of the internal or with inhibitor (must be suit- external cooling) able for copper) Approved for the food and ■...
  • Page 124 Multitron - Operating manual Technical Data The minimum rotation speed for all unit variants is 20 min . The max- imum rotation speed of an equipment unit depends on the throw and the position of the unit in the stack:...
  • Page 125 Multitron - Operating manual Technical Data Information on Temperatures that The temperature range limited by the measuring and control unit can Actually be Reached ranges from 4 to 65 °C. The temperatures that can actually be reached depend on a variety of factors, such as the ambient temperature, the ventilation and the temperature of the other devices in the stack.
  • Page 126 Multitron - Operating manual Technical Data 12.5 Specifications of the Options 12.5.1 Cooling Unit 900 W Electric Specifications Specification Type 230 Type 115 V Unit Value Max. power consumption Fuses (5 x 20 mm, time lag) Thermal protection switch Miscellaneous...
  • Page 127 Multitron - Operating manual Technical Data Miscellaneous Data Value Unit Cooling power 1800 Additional mass 80 kg Additional height 290 mm Cooling capacity at temperature of the cooling medium = 20 °C (20 °C ambient temperature) Mass including cooling coil and cover...
  • Page 128 Multitron - Operating manual Technical Data Requirements for the Cooling Liquid Based on 1,2-propanediol with inhibitor (must be suitable for ■ copper) Approved for the food and pharmaceutical sectors ■ Example: Antifrogen L ■ 12.5.4 Hygienic Humidity Control (HHC) Housing Dimensions...
  • Page 129 Multitron - Operating manual Technical Data Humidity that can be Reached Data Value Unit Max. humidity without conden- 80 % rH sation (at 37 °C in the incubation chamber , 20 °C ambient temp.) Max. humidity without conden- 85 % rH sation (at 37 °C in the incubation chamber ,...
  • Page 130 Multitron - Operating manual Technical Data Connection/Water Quality Data Value Unit Connection (hose Ø) 3.2 mm (UNF 1/4-28 for hoses 1/8") Input pressure -0.15 to 2 bar Water hardness (CaCO equiva- < 0.01 mmol/L lent) Dissolved solids < 10 mg/L...
  • Page 131 Multitron - Operating manual Technical Data Control Data Value Unit Control range 0.1 to 20 % CO Increment % CO Control precision 0.2 % CO (at 1013 hPa, 20 °C to 40 °C, 0 to 20 % CO2) 12.5.6 UV Decontamination...
  • Page 132 Multitron - Operating manual EU Declaration of Conformity 13 EU Declaration of Conformity Page 132 of 140 27.05.2024...
  • Page 133 Anschrift Address Adresse Konformitätsbeauftragter Representative for conformity Responsable de la conformité Bottmingen, 26. Jan. 2022 M. Heuschkel Ort, Datum Chief Technology Officer Place, date Lieu, date Doc. No. CER-CE-0003 Version Page 1 of 1 Company Confidential - © INFORS HT...
  • Page 134 Multitron - Operating manual Index 14 Index Clamps mount..........................76 Accessories.......................... 52 plastic..........................63 box for microtitre plates................64 stainless steel......................63 eve............................69 Cleaning Sticky Stuff........................59 base tray........................109 tray with clamping assembly.............. 56 basic unit........................108 tray with pin holders..................55 Sticky Stuff......................... 60 tray with sliding bars..................
  • Page 135 Multitron - Operating manual Index Device Exterior dimensions....................119 cleaning........................108 EXTERNAL ALARM......................31 deactivate........................95 External cooling system disinfect........................109 connecting......................... 40 dismantling........................ 113 functional description...................39 dispose..........................113 specification......................127 install..........................70 water quality......................127 loading........................75, 79 maintain........................107 positioning........................72 Fail failure..........................98 switch on........................74 Fan..............................30...
  • Page 136 Multitron - Operating manual Index operating........................43 Maximum rotation speeds................123 overview.........................41 with box for microtitre plates............66 Humidity sensor with Sticky Stuff....................62 position..........................41 Messages specification......................128 ALARM..........................97 Hygienic Humidity Control ERROR..........................99 faults..........................105 HEAT............................43 minimum distances..................72 HIGH............................ 97 specification......................128 RESTARTED.........................
  • Page 137 Multitron - Operating manual Index Overview accessories......................... 52 Safety features........................25 components......................... 9 Screw sizes display and operating elements............13 holders..........................78 exterior..........................9 shaking table......................109 interior..........................10 Sensor parameters........................12 ...........................45, 130 timer function......................81 humidity......................41, 128 Pt100........................30, 124 Serial number........................36 Parameter SET key............................15 deactivate........................81...
  • Page 138 Multitron - Operating manual Index Sticky Stuff maximum rotation speeds..............62 Universal table tray....................54 overview........................59 USB connection......................32 Sticky Stuff adhesive matting...............59 UV decontamination Storage.............................112 operating........................46 Substructure........................34 setup..........................46 Switch on..........................74 specification......................131 Switching key sound on/off................94 Switching off........................95 Symbols on the device......................26 Ventilation opening....................
  • Page 139 Multitron - Operating manual Index 27.05.2024 Page 139 of 140...
  • Page 140 Digitize your bioprocesses The platform software for your bioprocesses – the Bioprocess Platform Software ® Able to do more than just plan, control and analyze your bioprocesses, eve ® software integrates workflows, devices, bioprocess information and big data in a platform that lets you organize your projects in the cloud, no matter how complex they are.